Genetic manipulation of stationary-phase genes to enhance recombinant protein production in Escherichia coli

Biotechnology and Bioengineering

Volumn 50, Issue 6, 1996, Pages 636-642

  Chou, Chih Hsiung ^a,c   Bennett, George N ^b   San, Ka Yiu ^a  

^a Rice University   (United States)

^b RICE UNIVERSITY   (United States)

^c FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

Escherichia coli; genetic manipulation; recombinant protein; stationary phase genes

Indexed keywords

CELL PHYSIOLOGY; GENETIC MANIPULATION; RECOMBINANT PROTEIN ACTIVITY; RECOMBINANT PROTEIN PRODUCTION;

BACTERIA; BATCH CELL CULTURE; CELLS; CYTOLOGY; GENES; GROWTH KINETICS; PROTEINS;

GENETIC ENGINEERING;

BACTERIAL PROTEIN; BETA GALACTOSIDASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; ESCHERICHIA COLI; GENE EXPRESSION; GENE MUTATION; GENETIC MANIPULATION; NONHUMAN; PROTEIN SYNTHESIS; STRAIN DIFFERENCE;

EID: 15844391799     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960620)50:6<636::AID-BIT4>3.0.CO;2-L     Document Type: Article

References (49)

1. Aristidou, A. A., San, K.-Y., Bennett, G. N. 1994. Modification of central metabolic pathway in Escherichia coli to reduce acetate accumulation by heterologous expression of the Bacillus subtilis acetolactate synthase gene. Biotechnol. Bioeng. 44: 944-951.
2. + strains. J. Biotechnol. 29: 299-306.
3. Bentley, W. E., Mirjalili, N., Andersen, D. C., Davis, R. H., Kompala, D.S. 1990. Plasmid-encoded protein: The principal factor in the "metabolic burden" associated with recombinant bacteria. Biotechnol. Bioeng. 35: 668-681.
4. Chapman, A. G., Fall, L., Atkinson, D. E. 1971. Adenylate energy charge in Escherichia coli during growth and starvation. J. Bacteriol. 108: 1072-1086.
5. Chou, C.-H., Aristidou, A. A., Meng, S.-Y., Bennett, G. N., San, K.-Y. 1995. Characterization of a pH-inducible promoter system for high-level expression of recombinant proteins in Escherichia coli. Biotechnol. Bioeng., to appear.
6. Chou, C.-H., Bennett, G. N., San, K.-Y. 1994. Effect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in dense Escherichia coli cultures. Biotechnol. Bioeng. 44: 952-960.
7. Chou, C.-H., Bennett, G. N., San, K.-Y. 1994. Effect of modulated glucose uptake on high-level recombinant protein production in a dense Escherichia coli culture. Biotechnol. Prog. 10: 644-647.
8. Curless, C. E., Forrer, P. D., Mann, M. B., Fenton, D. M., Tsai, L. B. 1989. Chemoslat study of kinetics of human lymphokine synthesis in recombinant Escherichia coli. Biotechnol. Bioeng. 34:415-421.
9. Dedhia, N. N., Hottiger, T., Bailey, J.E. 1994. Overproduction of glycogen in Escherichia coli blocked in the acetate pathway improves cell growth. Biotechnol. Bioeng. 44: 132-139.
10. El-Mansi, E. M. T., Holms, W. H. 1989. Control of carbon flux to acetate excretion during growth of Escherichia coli in batch and continuous cultures. J. Gen. Microbiol. 135: 2875-2883.
11. Flickinger, M. C., Rouse, M. P. 1993. Sustaining protein synthesis in the absence of rapid cell division: An investigation of plasmid-encoded protein expression in Escherichia coli during very slow growth. Biotechnol. Prog. 9: 555-572.
12. s is positively regulated by ppGpp. J. Bacteriol. 175: 7982-7989.
13. Goeddel, D. V. (ed.). 1990. Methods in enzymology. vol. 185: Gene expression technology. Academic Press, San Diego, CA.
14. Han, K., Hong, J., Lim, H. C. 1993. Relieving effects of glycine and methionine from acetic acid inhibition in Escherichia coli fermentation. Biotechnol. Bioeng. 41: 316-324.
15. Han, K., Lim, H. C., Hong, J. 1992. Acetic acid formation in Escherichia coli fermentation. Biotechnol. Bioeng. 39: 663-671.
16. Holms, W. H. 1986. The central metabolic pathways of Escherichia coli: Relationship between flux and control at a branch point, efficiency of conversion to biomass, and excretion of acetate, pp. 69-105. In: B. L. Horecker, and E. R. Stadtman (eds.), Current topics in cellular regulation, vol. 28. Academic Press, Orlando, FL.
17. Huisman, G., Kolter, R. 1994. Regulation of gene expression at the onset of stationary phase in Escherichia coli, pp. 21-40. In: P. Piggot, et al. (eds.), Regulation of bacterial differentiation. American Society for Microbiology, Washington, D.C.
18. Ingraham, J. L., Maaloe, O., Neidhardt, F. C. 1983. Growth of the bacterial cell. Sinauer Associates, Sunderland, MA.
19. Kennel, D. E. 1986. The instability of message RNA in bacteria, pp. 101-142. In: W. Reznikoff and L. Gold (eds.), Maximizing gene expression. Butterworth Publishers, Stoneham, MA.
20. Kim, J.-Y., Ryu, D. D. Y. 1991. The effects of plasmid content, transcription efficiency, and translation efficiency on the productivity of a cloned gene protein in Escherichia coli. Biotechnol. Bioeng. 38: 1271-1279.
21. Lange, R., Hengge-Aronis, R. 1991. Identification of a central regulator of stationary-phase gene expression in Escherichia coli. Mol. Microbiol. 5: 49-59.
22. Leipold, R. J., Dhurjati, P. 1993. Construction and characterization of a specialized ribosome system for the overproduction of proteins in Escherichia coli. Biotechnol. Prog. 9: 345-354.
23. Loewen, P. C., Triggs, B. L. 1984. Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli. J. Bacteriol. 160: 668-675.
24. Luli, G. W., Strohl, W. R. 1990. Comparison of growth, acetate production and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl. Environ. Microbiol. 56: 1004-1011.
25. MacDonald, H. L., Neway, J. O. 1990. Effects of medium quality on the expression of human interleukin-2 at high cell density in fermentor cultures of Escherichia coli K-12. Appl. Environ. Microbiol. 56: 640-645.
26. Maniatis, T., Fritsch, E. F., Sambrook, J. 1982. Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
27. Mason, C. A., Bailey, J. E. 1989. Effects of plasmid presence on growth and enzymatic activity in Escherichia coli DH5α. Appl. Microbiol. Biotechnol. 32: 54-60.
28. Meng, S.-Y. 1991. Studies on the cad operon of Escherichia coli K-12: A pH-regulatory system in bacteria, Ph.D. thesis, Rice University, Houston, TX.
29. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor. NY.
30. Moser, A. 1991. Tubular bioreactors: Case study of bioreactor performance for industrial production and scientific research. Biotechnol. Bioeng. 37: 1054-1065.
31. Nguyen, L. H., Jensen, D. B., Thompson, N. E., Gentry, D. R., Burgess, R. R. 1993. In vitro functional characterization of over-produced Escherichia coli katF/rpoS gene product. Biochemistry-USA 32: 11112-11117.
32. Odgen, K. L., Davis, R. H., Taylor, A. L. 1992. An adjustable expression system for controlling growth rate, plasmid maintenance, and culture dynamics. Biotechnol. Bioeng. 40: 1027-1038.
33. Ogden, K. L., Davis, R. H. 1991. Plasmid maintenance and protein overproduction in selective recycle bioreactors. Biotechnol. Bioeng. 37: 325-333.
34. Peretti, S. W., Bailey, J. E., Lee, J. J. 1988. Transcription from plasmid genes, macromolecular stability, and cell-specific productivity in Escherichia coli carrying copy number mutant plasmids. Biotechnol. Bioeng. 34: 902-908.
35. Petersen, C. 1987. The functional stability of the lacZ transcript is sensitive towards sequence alterations immediately downstream of the ribosome binding site. Mol. Gen. Genet. 209: 179-187.
36. Ramírez, D. M., Bentley, W. E. 1993. Enhancement of recombinant protein synthesis and stability via coordinated amino acid addition. Biotechnol. Bioeng. 41: 557-565.
37. Ryan, W., Parulekar, S. J. 1991. Recombinant protein synthesis and plasmid instability in continuous cultures of Escherichia coli JM103 harboring a high copy number plasmid. Biotechnol. Bioeng. 37: 415-425.
38. Schoner, B. E., Hsiung, H. M., Belagaje, R. M., Mayne, N. G., Schoner, R. G. 1984. Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli. Proc. Natl. Acad. Sci. USA 81: 5403-5407.
39. Schroeckh, V., Hartmann, M., Hirschfeld, E. B., Riesenberg, D. 1992. Improvement of recombinant gene expression in Escherichia coli for glucose-controlled continuous and fed-batch cultures. Appl. Environ. Microbiol. 36: 487-492.
40. Schweizer, H. P. 1993. Two plasmids, X1918 and Z1918, for easy recovery of the xylE and lacZ reporter genes. Gene 134: 89-91.
41. Seo, J.-H., Bailey, J. E. 1985. Effects of recombinant plasmid content on growth properties and cloned gene product formation in Escherichia coli. Biotechnol. Bioeng. 27: 1668-1674.
42. Siegele, D. A., Kolter, R. 1992. Minireview: Life after log. J. Bacteriol. 174: 345-348.
43. Tessier, L.-H., Sondermeyer, P., Faure, T., Dreyer, D., Benavente, A., Villeval, D., Courtney, M., Lecocq, J.-P. 1984. The influence of mRNA primary and secondary structure on human IFN-γ gene expression in E. coli. Nucleic Acids Res. 12: 7663-7675.
44. Thomas, D. Y., Dubuc, G., Narang, S. 1982. Escherichia coli plasmid vectors containing synthetic translational initiation sequences and ribosome binding sites fused with the lacZ gene. Gene 19: 211-219.
45. Tolentino, G. J., Meng, S.-Y., Bennett, G. N., San, K.-Y. 1992. A pH-regulated promoter for the expression of recombinant proteins in Escherichia coli. Biotechnol. Lett. 14: 157-162.
46. Vind, J., Sorensen, M. A., Rasmussen, M. D., Pedersen, S. 1993. Synthesis of proteins in Escherichia coli is limited by the concentration of free ribisomes: Expression from reporter genes does not always reflect functional mRNA levels. J. Mol. Biol. 231: 678-688.
47. Wada, A., Yamazaki, Y., Fujita, N., Ishihama, A. 1990. Structure and probable genetic location of a "ribosome modulation factor" associated with 100S ribosomes in stationary-phase Escherichia coli cells. Proc. Natl. Acad. Sci. USA 87: 2657-2661.
48. Yamagishi, M., Matsushima, H., Wada, A., Sakagami, M., Fujita, N., Ishihama, A. 1993. Regulation of the Escherichia coli rmf gene encoding the ribosome modulation factor: Growth phase- and growth rate-dependent control. EMBO J. 12: 625-630.
49. Zabriskie, D. W., Arcuri, E. J. 1986. Factors influencng productivity of fermentations employing recombinant microorganisms. Enzyme Microb. Technol. 8: 706-717.