Size distribution in ultraphytoplankton: A comparative analysis of counting methods

Environmental Monitoring and Assessment

Volumn 102, Issue 1-3, 2005, Pages 85-101

  Pitta, P ^a   Karakassis, I ^a,b  

^a INSTITUTE OF OCEANOGRAPHY   (Greece)

^b TECHNICAL UNIVERSITY OF CRETE   (Greece)

Author keywords

Comparison of methods; Direct observation DAPI staining; Separate filtration; Sequential filtration; Size fractionation; Ultraphytoplankton

Indexed keywords

BACTERIA; FILTERS (FOR FLUIDS); FILTRATION; PHOTOSYNTHESIS; PORE SIZE; SEAWATER;

CYANOBACTERIA; FLAGELLATES; SIZE FRACTIONATION; ULTRAPHYTOPLANKTON;

MARINE BIOLOGY;

4',6 DIAMIDINO 2 PHENYLINDOLE;

MARINE ENVIRONMENT; OLIGOTROPHIC ENVIRONMENT; PHYTOPLANKTON; SAMPLING; SIZE DISTRIBUTION;

ARTICLE; BIOFILTER; BIOFILTRATION; COMPARATIVE STUDY; CONTROLLED STUDY; CYANOBACTERIUM; FLAGELLATE; FRACTIONATION; GREECE; INTERMETHOD COMPARISON; NONHUMAN; OBSERVATION; ORGANISM GROUP SIZE; PHOTOTROPHIC BACTERIUM; PHYTOPLANKTON; SEASHORE; SEPARATION TECHNIQUE; SEQUENTIAL ANALYSIS; STAINING; STATISTICAL DISTRIBUTION; STATISTICAL SIGNIFICANCE; WATER SAMPLING;

ANALYSIS OF VARIANCE; ANIMALS; CELL COUNT; CELL SIZE; COLONY COUNT, MICROBIAL; CYANOBACTERIA; DIATOMS; DINOFLAGELLIDA; FILTRATION; FLUORESCENT DYES; INDOLES; MICROPORE FILTERS; OCEANS AND SEAS; PHYTOPLANKTON;

CRETAN SEA; MEDITERRANEAN SEA; OCEANIC REGIONS; WORLD;

CYANOBACTERIA; MASTIGOPHORA (FLAGELLATES);

EID: 15744377135     PISSN: 01676369     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10661-005-2690-5     Document Type: Article

References (56)

1. Acosta Pomar, L., Bruni, V., Decembrini, F., Giuffre, G. and Maugeri T.L.: 1988, 'Distribution and activity of picophytoplankton in a brackish environment' Deep-Sea Res. 21, 129-138.
2. Booth, B.C., Lewin, J. and Norris, R.E.: 1982, 'Nanoplankton species predominant in the subarctic Pacific in May and June 1978' Deep-Sea Res. 29, 185-200.
3. B. C. Booth 1993 'Estimating Cell Concentration and Biomass of Autotrophic Plankton Using Microscopy' in: P. F. Kemp B. F. Sherr E. B. Sherr J. J. Cole (eds) Handbook of Methods in Aquatic Microbial Ecology Lewis Publishers Boca Raton 199-205
4. Caron, D.A.: 1983, 'Technique for enumeration of heterotrophic and phototrophic nanoplankton, using epifluorescence microscopy, and comparison with other procedures', Appl. Environ. Microbiol. 46, 491-498.
5. Chepurnova, E.A. and Gutveyb, L.G.: 1988, 'Distribution and size structure of phototrophic bacterioplankton (Cyanobacteria) in the Mediterranean Sea and Atlantic Ocean' Oceanology 28, 643-647.
6. Chisholm, S.W., Olson, R.J., Zettler, E.R., Goericke, R., Waterbury, J.B. and Welschmeyer, N.A.: 1988, 'A novel free-living prochlorophyte abundant in the oceanic euphotic zone', Nature 334, 340-343.
7. Christaki, U., Van Wambeke, F. and Dolan, J.R.: 1999, 'Nanoflagellates (mixotrophs, heterotrophs and autotrophs) in the oligotrophic eastern Mediterranean: Standing stocks, bacterivory and relationships with bacterial production' Mar. Ecol. Prog. Ser. 181, 297-307.
8. Davis, P.G., Caron, D.A., Johnson, P.W. and Sieburth, J.McN.: 1985, 'Phototrophic and apochlorotic components of picoplankton and nanoplankton in the North Atlantic: Geographic, vertical, seasonal and diel distributions' Mar. Ecol. Prog. Ser. 21, 15-26.
9. DuRand, M.D. and Olson, R.J.: 1996, 'Conributions of phytoplankton light scattering and cell concentration changes to diel variations in beam attenuation in the equatorial Pacific from flow cytometric measurements of pico-, ultra- and nanoplankton' Deep-Sea Res. II 43, 891-906.
10. Ferrier-Pag̀s, C. and Gattuso, J.P.: 1998, 'Biomass, production and grazing rates of pico- and nanoplankton in coral reef waters (Miyako Island, Japan)', Microb. Ecol. 35, 46-57.
11. Glover, H.E., Campbell, L. and Prezelin, B.B.: 1986, 'Contribution of Synechococcus spp. to size-fractioned primary productivity in three water masses in the Northwest Atlantic Ocean', Mar. Biol. 91, 193-203.
12. Glover, H.E., Prezelin, B., Campbell, L. and Wyman, M.: 1988, 'Pico- and ultraplankton Sargasso Sea communities: Variability and comparative distributions of Synechococcus spp. and algae', Mar. Ecol. Prog. Ser. 49, 127-139.
13. Glover, H.E., Smith, A.E. and Shapiro, L.: 1985, 'Diurnal variations in photosynthetic rates: Comparisons of ultraphytoplankton with a larger phytoplankton size fraction', J. Plankton Res. 7, 519-535.
14. Goericke, R. and Welschmeyer, N.A.: 1993, 'The marine prochlorophyte Prochlorococcus contributes significantly to phytoplankton biomass and primary production in the Sargasso Sea', Deep-Sea Res. 40, 2283-2294.
15. Goldman, J.C. and Dennett, M.R.: 1985, 'Susceptibility of some marine phytoplankton species to cell breakage during filtration and post-filtration rinsing', J. Exp. Mar. Biol. Ecol. 86, 47-58.
16. Guillou, L., Jacquet, S., Chrétiennot-Dinet, M.-J. and Vaulot, D.: 2001, 'Grazing impact of the small heterotrophic flagellates on Prochlorococcus and Synechococcus', Aquat. Microb. Ecol. 26, 201-207.
17. Hadas, O. and Berman, T.: 1998, 'Seasonal abundance and vertical distribution of Protozoa (flagellates, ciliates) and bacteria in Lake Kinneret, Israel', Aquat. Microb. Ecol. 14, 161-170.
18. Hannah, F.J. and Boney, A.D.: 1983, 'Nanophytoplankton in the Firth of Clyde, Scotland: Seasonal abundance, carbon fixation and species composition', J. Exp. Mar. Biol. Ecol. 67, 105-147.
19. Havskum, H. and Hansen, A.S.: 1997, 'Importance of pigmented and colourless nano-sized protists as grazers on nanoplankton in a phosphate-depleted Norwegian fjord and in enclosures', Aquat. Microb. Ecol. 12, 139-151.
20. Hobbie, J.E., Daley, R.J. and Jasper, S.: 1977, 'Use of Nuclepore filters for counting bacteria by fluorescence microscopy' Appl. Environ. Microbiol. 33, 1225-1228.
21. Iriarte, A.: 1993, 'Size-fractionated chlorophyll a biomass and picophytoplankton cell density along a longitudinal axis of a temperate estuary (Southampton Water)', J. Plankton Res. 15, 485-500.
22. Iriarte, A. and Purdie, D.A.: 1994, 'Size distribution of chlorophyll a biomass and primary production in a temperate estuary (Southampton Water): The contribution of photosynthetic picoplankton', Mar. Ecol. Prog. Ser. 115, 283-297.
23. Jacquet, S., Lennon, J.-F., Marie, D. and Vaulot, D.: 1998, 'Picoplankton population dynamics in coastal waters of the northwestern Mediterranean Sea', Limnol. Oceanogr. 43, 1916-1931.
24. Johnson, P.W. and Sieburth, J.M.: 1979, 'Chroococcoid cyanobacteria in the sea: A ubiquitous and diverse phototrophic biomass' Limnol. Oceanogr. 24, 928-935.
25. Joint, I.R., Owens, N.J.P. and Pomroy, A.J.: 1986, 'Seasonal production of photosynthetic picoplankton and nanoplankton in the Celtic Sea', Mar. Ecol. Prog. Ser. 28, 251-258.
26. Kirchman, D.L., Rich, J.H. and Barber, R.T.: 1995, 'Biomass and production of heterotrophic bacteria along 140W in the equatorial Pacific: Effect of temperature on the microbial loop' Deep Sea Res. 42, 603-619.
27. Kuosa H. 1988 'Occurrence of autotrophic picoplankton along an open sea-Inner archipelago gradient in the Gulf of Finland, Baltic Sea' Ophelia 28 85-93
28. Kuylenstierna, M. and Karlson, B.: 1994, 'Seasonality and composition of pico- and nanoplanktonic cyanobacteria and protists in the Skagerrak', Bot. Mar. 37, 17-33.
29. Li, W.K.W.: 1990, 'Particles in "particle-free" seawater: Growth of ultraphytoplankton and implications for dilution experiments', Can. J. Fish Aquat. Sci. 47, 1258-1268.
30. Li, W.K.W., Subba Rao, D.V., Harrison, W.G., Smith, J.C., Cullen, J.J., Irwin, B. and Platt, T.: 1983, 'Autotrophic picoplankton in the tropical ocean' Science 219, 292-295.
31. Li, W.K.W., Zohary, T., Yacobi, Y.Z. and Wood, A.M.: 1993, 'Ultraphytoplankton in the eastern Mediterranean Sea: Towards deriving phytoplankton biomass from flow cytometric measurements of abundance, fluorescence and light scatter', Mar. Ecol. Prog. Ser. 102, 79-87.
32. Logan, B.E.: 1993, 'Theoretical analysis of size distributions determined with screens and filters' Limnol. Oceanogr. 38, 372-381.
33. Logan, B.E., Passow, U. and Alldredge, A.L.: 1994, 'Variable retention of diatoms on screens during size separations' Limnol. Oceanogr. 39, 390-395.
34. Magazzu, G., Bruni, V., Piccione, A., Platt, T., Irwin, B. and Rao, D.V.S.: 1987, 'Picoplankton: Contribution to phytoplankton production in the strait of Messina', PSZNI: Mar. Ecol. 8, 21-31.
35. T. C. Malone 1980 'Algal Size' in: I. Morris (ed.) The Physiological Ecology of Phytoplankton Blackwell Scientific Publications London 1-625
36. Marshall, H.G.: 1995, 'Autotrophic picoplankton distribution and abundance in the Chesapeake Bay, U.S.A', Mar. Nat. 4, 33-42.
37. Maugeri, T.L., Acosta Pomar, M.L.C., Bruni, V. and Salomone, L.: 1992, 'Picoplankton and picophytoplankton in the Ligurian Sea and straits of Messina (Mediterranean Sea)' Bot. Mar. 35, 493-502.
38. Murphy, L.S. and Haugen, E.M.: 1985, 'The distribution and abundance of phototrophic ultraplankton in the North Atlantic', Limnol. Oceanogr. 30, 47-58.
39. Partensky, F., Blanchot, J., Lantoine, F., Neveux, J. and Marie, D.: 1996, 'Vertical structure of picophytoplankton at different trophic sites of the tropical northeastern Atlantic Ocean', Deep-Sea Res. I 43, 1191-1213.
40. Platt, T., Rao, D.V.S. and Irwin, B.: 1983, 'Photosynthesis of picoplankton in the oligotrophic ocean', Nature 300, 702-704.
41. Porter, K.G. and Feig, Y.S.: 1980, 'The use of DAPI for identifying and counting aquatic microflora', Limnol. Oceanogr. 25, 943-948.
42. Quinones, R.A.: 1994, 'A comment on the use of allometry in the study of pelagic ecosystem processes' Sci. Mar. 58, 11-16.
43. Reynolds, N.: 1973, 'The estimation of the abundance of ultraplankton', Br. Phycol. J. 8, 135-146.
44. Sanders, R.W., Berninger, U.G., Lim, E.L., Kemp, P.F. and Caron, D.A.: 2000, 'Heterotrophic and mixotrophic nanoplankton predation on picoplankton in the Sargasso Sea and Georges Bank', Mar. Ecol. Prog. Ser. 192, 103-118.
45. Sheldon, R.W. and Sutcliffe, W.H. Jr.: 1969, 'Retention of marine particles by screens and filters', Limnol. Oceanogr. 14, 441-444.
46. Sheldon, R.W.: 1972, 'Size separation of marine seston by membrane and glass-fiber filters', Limnol. Oceanogr. 17, 494-498.
47. Shimada, A., Hasegawa, T., Umeda, I., Kadoya, N. and Maruyama, T.: 1993, 'Spatial mesoscale patterns of West Pacific picophytoplankton as analyzed by flow cytometry: Their contribution to subsurface chlorophyll maxima', Mar. Biol. 115, 209-215.
48. Sieburth, J. McN., Smetacek, V. and Lenz, J.: 1978, 'Pelagic ecosystem structure: Heterotrophic compartments of the plankton and their relationship to plankton size fractions', Limnol. Oceanogr. 23, 1256-1263.
49. Stockner, J. G., Klut, M. E. and Cochlan, W. P.: 1990, 'Leaky filters: A warning to aquatic ecologists', Can. J. Fish Aquat. Sci. 47, 16-23.
50. Utermöhl, H.: 1958, 'Zur Vervollkommnung der quantitativen Phytoplankton-methodik' Ecol. Monogr. 57, 61-88.
51. Vaulot, D., Partensky, F., Neveux, J., Mantoura, R. F. C. and Llewellyn, C.: 1990, 'Winter presence of prochlorophytes in surface waters of the north-western Mediterranean Sea', Limnol. Oceanogr. 35, 1156-1164.
52. Waterbury, J.B., Watson, S.W., Guillard, R.R.L. and Brand, L.E.: 1979, 'Widespread occurrence of a unicellular, marine, planktonic cyanobacterium', Nature 277, 293-294.
53. Williams, R.B.: 1964, 'Division rates of salt marsh diatoms in relation to salinity and cell size', Ecology 45, 877-880.
54. Zaika, V.Y.: 1986, 'Vertical distribution of autotrophic picoplankton in the Indian Ocean and the Mediterranean Sea', Oceanology 26, 209-212.
55. Zaika, V.E. and Yashin, V.A.: 1984, 'Lumenescing picosuspension (0.2-2 μm) in oligotrophic waters of the Mediterranean and Black seas', Dokl. Biol. Sci. 275, 262-264.
56. Zimmermann, R. and Meyer-Reil, L.A.: 1974, 'A new method for fluorescence staining of bacterial populations on membrane filters', Kieler Meeresforsch. 30, 24-27.