Molecular structure of agarose chains in thermoreversible hydrogels revealed by means of a fluorescent probe technique

Journal of Polymer Science, Part B: Polymer Physics

Volumn 43, Issue 6, 2005, Pages 680-688

  Itagaki, Hideyuki ^a   Fukiishi, Hiroyasu ^a   Imai, Tomoe ^a   Watase, Mineo ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

Author keywords

Fluorescence; Gels; Helical structure

Indexed keywords

FLUORESCENCE; GELS; HYDROPHOBICITY; INTERCALATION COMPOUNDS; MOLECULAR STRUCTURE; POLYSACCHARIDES; X RAY DIFFRACTION ANALYSIS;

AGAROSE FIBERS; FLUORESCENT PROBE TECHNIQUE; HELICAL STRUCTURES; POLYMER CONCENTRATION;

HYDROGELS;

EID: 15544375667     PISSN: 08876266     EISSN: None     Source Type: Journal    
DOI: 10.1002/polb.20374     Document Type: Article

References (30)

1. Indovina, P. L.; Tettamanti, E.; Micciancio-Giammarinaro, M. S.; Palma, M. U. J Chem Phys 1979, 70, 2841.
2. Nishinari, K.; Watase, M. Carbohydr Polym 1983, 3, 39.
3. Tokita, M.; Hikichi, K. Phys Rev 1987, A35, 4329.
4. Watase, M.; Nishinari, K.; Clark, A. H.; Ross-Murphy, S. B. Macromolecules 1989, 22, 1196.
5. Watase, M.; Nishinari, K.; Williams, P. A.; Phillips, G. O. J Agric Food Chem 1990, 38, 1181.
6. For instance: Robert, M. C.; Provost, K.; Lefaucheux, F. In Crystallization of Nucleic Acids and Proteins; Oxford University Press: Oxford, 1992; p. 127.
7. Arnott, S.; Fulmer, A.; Scott, W. E.; Dea, I. C. M.; Moorhouse, R.; Rees, D. A. J Mol Biol 1974, 90, 269.
8. Anderson, N. S.; Campbell, J. W.; Harding, M. M.; Rees, D. A.; Samuel, J. W. B. J Mol Bio 1969, 45, 85.
9. Arnott, S.; Fulmer, A.; Scott, W. E.; Rees, D. A.; McNab, G. C. A. J Mol Biol 1974, 90, 253.
10. Foord, S. A.; Atkins, E. D. T. Biopolymers 1989, 28, 1345.
11. Rochas, C.; Brulet, A.; Guenet, J. M. Macromolecules 1994, 27, 3830.
12. Ramzi, M.; Rochas, C.; Guenet, J. M. Macromolecules 1998, 31, 6106.
13. Ramzi, M.; Rochas, C.; Guenet, J. M. Macromolecules 1996, 29, 4668.
14. Guenet, J. M.; Dahmani, M.; Ramzi, M.; Rochas, C. Macromol Symp 2001, 166, 139.
15. Itagaki, H.; Horie, K.; Mita, I. Prog Polym Sci 1990, 15, 361.
16. Itagaki, H. In Experimental Methods in Polymer Science: Modern Methods in Polymer Research and Technology; Tanaka, T., Ed.; Academic Press: New York, 2000; Chapter 3, p. 155.
17. Itagaki, H. Macromol Symp 2001, 166, 13.
18. Weber, G.; Laurence, D. J. R. Biochem J 1954, 56, xxxi.
19. Stryer, L. J Mol Biol 1965, 13, 482.
20. Turner, D. C.; Brand, L. Biochemistry 1968, 7, 3381.
21. Watase, M.; Nishinari, K. Makromol Chem 1987, 188, 1177.
22. Dahmani, M.; Ramzi, M.; Rochas, C.; Guenet, J. M. Int J Biol Macromol 2003, 31, 147.
23. Itagaki, H.; Nakazawa, R.; Watase, M. Proceedings of IUPAC MACRO 2000, 2000, 463.
24. We used a value of 0.63 as the fluorescence quantum yield of ANS in non-polar circumstances, after ref. 19.
25. Nearly the same values of standard deviation were given for the combination of two components under polar circumstances and one component under a quite non-polar one. Then, we determined the best fitting curves under the condition where two fluo rescent components with peaks at 461 and 542 nm were fixed.
26. We showed the best fitting, but some had nearly the same values of standard deviation. We think that the wavelength of the third component observed in HAG hydrogels is around 510 nm in common.
27. Jimenez-Barbero, J.; Bouffar-Roupe, C.; Rochas, C.; Pérez, S. Int J Biol Macromol 1989, 11, 265.
28. Sugiyama, J.; Rochas, C.; Turquois, T.; Taravel, F.; Chanzy, H. Carbohydr Polym 1994, 23, 261.
29. Ramzi, M.; Rochas, C.; Guenet, J. M. Int J Biol Macromol 2000, 27, 163.
30. All we can deduce here is the existence of a loose helical structure different from the Helix I form.