The preparation of HEK293 α1A or HEK293 α1B cell membrane stationary phase and the chromatographic affinity study of ligands of α1 adrenoceptor

Analytical Biochemistry

Volumn 339, Issue 2, 2005, Pages 198-205

  Yu, Wang ^a   Bingxiang, Yuan ^a   Xiuling, Deng ^a   Langchong, He ^a   Youyi, Zhang ^b   Qide, Han ^b  

^a XI'AN JIAOTONG UNIVERSITY   (China)

^b PEKING UNIVERSITY   (China)

Author keywords

Cell membrane chromatography; Cell membrane stationary phase; Drug; Human embryonic kidney 293 cell lines; Immobilization; Liquid chromatography; Membrane; Receptor; 1A Adrenergic receptors; 1B Adrenergic receptors

Indexed keywords

CELLS; CLONING; LIGANDS; LIQUID CHROMATOGRAPHY; MEMBRANES; RADIOACTIVE WASTE VITRIFICATION; SILICA;

ADRENERGIC RECEPTORS; CELL MEMBRANE CHROMATOGRAPHIES; CELL MEMBRANE STATIONARY PHASE; DRUG; HUMAN EMBRYONIC KIDNEY 293 CELLS; RECEPTOR;

CELL IMMOBILIZATION;

ALPHA 1A ADRENERGIC RECEPTOR; ALPHA 1B ADRENERGIC RECEPTOR; COMPLEMENTARY DNA; SILICON DIOXIDE; ADRENERGIC RECEPTOR ALPHA(1A); ADRENERGIC RECEPTOR ALPHA(1B); ALPHA 1 ADRENERGIC RECEPTOR; ALPHA ADRENERGIC RECEPTOR BLOCKING AGENT; ALPHA ADRENERGIC RECEPTOR STIMULATING AGENT; LIGAND;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; CORRELATION ANALYSIS; EMBRYO; FEASIBILITY STUDY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMERSION; IMMOBILIZED CELL; MATHEMATICAL ANALYSIS; MOLECULAR CLONING; MOLECULAR INTERACTION; MOLECULAR MODEL; POROSITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SURFACE PROPERTY; CELL FRACTIONATION; CELL LINE; COMPARATIVE STUDY; CYTOLOGY; KIDNEY; METABOLISM; METHODOLOGY; PRENATAL DEVELOPMENT;

ADRENERGIC ALPHA-AGONISTS; ADRENERGIC ALPHA-ANTAGONISTS; CELL FRACTIONATION; CELL LINE; CELL MEMBRANE; CHROMATOGRAPHY, AFFINITY; HUMANS; KIDNEY; LIGANDS; RECEPTORS, ADRENERGIC, ALPHA-1;

EID: 15444378195     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2005.01.004     Document Type: Article

References (21)

1. Zhang Yanxiao, Xiao Yingxian, K.J. Kellar, I.W. Wainer, Immobilized nicotinic receptor stationary phase for on-line liquid chromatographic determination of drug-receptor affinities, Anal. Biochem. 264 (1998) 22-24
2. He Langchong, Yang Guangde, Geng Xindu, Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface, Chin. Sci. Bull. 44 (1999) 826-831
3. He Langchong, Wang Sicen, Geng Xindu, Coating and fusing cell membrane on silica surface and its chromatographic characteristics, Chromatographia 54 (2001) 71-76
4. D.B. Bylund, D.C. Eikenberg, J.P. Hieble, S.Z. Langer, R.J. Lefkowitz, K.P. Minneman, P.B. Molinoff, R.R. Ruffolo Jr., and U. Trendelenburg International Union of Pharmacology nomenclature of adrenoceptors Pharmacol. Rev. 46 1994 121 136
5. J.P. Hiebler Adrenoceptor subclassification: An approach to improved cardiovascular therapeutics Pharm. Acta Helv. 74 2000 163 171
6. L.M. Eri, and K.J. Tveter alpha-Blockade in the treatment of symptomatic benign prostatic hyperplasia J. Urol. 154 1995 923 934
7. A. Cavalli, A.L. Lattion, E. Hummler, M. Nenniger, T. Pedrazzini, J.F. Aubert, M.C. Michel, M. Yang, G. Lembo, C. Vecchione, M. Mostardini, A. Schmidt, F. Beermann, and S. Cotecchia Decreased blood pressure response in mice deficient of the alpha-1b adrenergic receptor Proc. Natl. Acad. Sci. USA 94 1997 11589 11594
8. Hou Jin, Yuan Bingxiang, He Langchong, Yang Guangde, Mi Man, Evaluation of drug-muscarinic receptor affinities by using cell membrane chromatography, Chin. J. Pharmacol. Toxicol. 17 (2003) 70-73
9. Zhao Huiru, Yang Guangde, He Langchong, Screening the effective component of Angelica sinensis by cell membrane chromatography, Chin. Pharm. J. 35 (2000) 13-15
10. Zhao Xiaojuan, Dang Gaochao, Yang Guangde, He Langchong, Analysis and comparation of the effective component in the roots and leaves of Herba eplmedii, Chin. J. Anal. Chem. 30 (2002) 195-197
11. Gao Kun, He Langchong, Yang Guangde, Screening the effective component of Leontice robustum by cell membrane chromatography, Chin. Pharm. J. 38 (2003) 15-16
12. Zhang Hanli, Yang Guangde, He Langchong, Yang Yinjing, Studies on screening the effective components of Cladonia alpestris and its correlation with pharmacological effects, Chin. Pharm. J. 38 (2003) 92-94
13. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248
14. Lei Beilei, Zhang Youyi, Han Chide, Sustained norepinephrine stimulation induces different regulation of expression in three alpha1-adrenoceptor subtypes, Life Sci. 69 (2001) 301-308
15. 1 D subtype Eur. J. Pharmacol. 297 1996 241 248
16. 1-adrenoceptor subtypes Naunyn Schmiedebergs Arch. Pharmacol. 350 1994 136 142
17. Rodolfo Testa, Carla Destefani, Luciano Guarneri, Elena Poggesi, Iris Simonazzi, Carlo Taddei, Amedeo Leonardi, The alpha1d-adrenoceptor subtype is involved in the noradrenaline-induced contractions of rat aorta, Life Sci. 57 (1995) P159-P163
18. Zhang Youyi, Xu Kaiming, Han Chide, alpha1 adrenoceptor subtypes mediating inotropic responses in rat heart, J. Pharmacol. Exp. Ther. 291 (1999) 829-836
19. E.M. Ross Pharmacodynamics: Mechanisms of drug action and the relationship between drug concentration and effects 1996 McGraw-Hill San Francisco
20. I.W. Wainer Enantioselective high performance liquid affinity chromatography as a probe of ligand-biopolymer interactions: An overview of a different use for HPLC chiral stationary phase J. Chromatogr. B 666 1994 221 234
21. E. Brekkan, A. Lundqvist, and P. Lundahl Immobilized membrane vesicle or proteoliposome affinity chromatography. Frontal analysis of interactions of cytochalasin B and d-glucose with the human red cell glucose transporter Biochemistry 35 1996 141 145