Automated acquisition of cryo-electron micrographs for single particle reconstruction on an FEI Tecnai electron microscope

Journal of Structural Biology

Volumn 150, Issue 1, 2005, Pages 69-80

  Lei, Jianlin ^a   Frank, Joachim ^a,b,c  

^a WADSWORTH CENTER   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

Automation; Cryo electron microscopy

Indexed keywords

ARTICLE; AUTOMATION; COMPUTER INTERFACE; COMPUTER PROGRAM; COMPUTER SYSTEM; CRYOELECTRON MICROSCOPY; DATA ANALYSIS; DATA BASE; ELECTRON MICROSCOPE; IMAGE QUALITY; IMAGING; INTERMETHOD COMPARISON; MONITORING; PERFORMANCE; PRIORITY JOURNAL; PROCESS CONTROL; QUALITATIVE ANALYSIS; SPECTRUM; SYSTEM ANALYSIS;

EID: 15444370401     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsb.2005.01.002     Document Type: Article

References (14)

1. B. Carragher, N. Kisseberth, D. Kriegman, R.A. Milligan, C.S. Potter, J. Pulokas, and A. Reilein Leginon: An automated system for acquistion of images from vitreous ice specimens J. Struct. Biol. 132 2000 33 45
2. K. Dierksen, D. Typke, R. Hegerl, A.J. Koster, and W. Baumeister Towards automatic electron tomography Ultramicroscopy 40 1992 71 87
3. K. Dierksen, D. Typke, R. Hegerl, and W. Baumeister Towards automatic electron tomography. II. Implementation of autofocus and low-dose procedures Ultramicroscopy 49 1993 109 120
4. R. Eusemann, H. Rose, and J. Dubochet Electron scattering in ice and organic materials J. Microsc. 128 1982 239 247
5. J. Frank Three-dimensional electron microscopy of macromolecular assemblies 1996 Academic Press San Diego
6. J.C. Fung, W. Liu, W.J. De Ruijter, H. Chen, C.K. Abbey, J.W. Sedat, and D.A. Agard Toward fully automated high-resolution electron tomography J. Struct. Biol. 116 1996 181 189
7. R. Henderson The potential and limitations of neutrons, electrons, and X-rays for atomic resolution microscopy of unstained biological macromolecules Q. Rev. Biophys. 28 1995 171 193
8. A.J. Koster, H. Chen, J.W. Sedat, and D.A. Agard Automated microscopy for electron tomography Ultramicroscopy 46 1992 207 227
9. J. Lepault, F.P. Booy, and J. Dubochet Electron microscopy of frozen biological suspensions J. Microsc. 129 1983 89 102
10. F. Mouche, Y. Zhu, J. Pulokas, C.S. Potter, and B. Carragher Automated three-dimensional reconstruction of keyhole limpet hemocyanin type 1 J. Struct. Biol. 144 2003 301 312
11. C.S. Potter, H. Chu, B. Frey, C. Green, N. Kisseberth, T.J. Madden, K.L. Miller, K. Nahrstedt, J. Pulokas, A. Reilein, D. Tcheng, D. Weber, and B. Carragher Leginon: A system for fully automated acquisition of 1000 micrographs a day Ultramicroscopy 77 1999 153 161
12. I. Rouiller, J. Pulokas, V.M. Butel, R.A. Milligan, E.M. Wilson-Kubalek, C.S. Potter, and B.O. Carragher Automated image acquisition for single-particle reconstruction using p97 as the biological sample J. Struct. Biol. 133 2001 102 107
13. P. Zhang, A. Beatty, J.L. Milne, and S. Subramaniam Automated data collection with a Tecnai 12 electron microscope: Applications for molecular imaging by cryomicroscopy J. Struct. Biol. 135 2001 251 261
14. P. Zhang, M.J. Borgnia, P. Mooney, D. Shi, M. Pan, P. O'Herron, A. Mao, D. Brogan, J.L.S. Milne, and S. Subramaniam Automated image acquisition and processing using a new generation of 4K × 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies J. Struct. Biol. 143 2003 134 144