The Drosophila gene abnormal spindle encodes a novel microtubule- associated protein that associates with the polar regions of the mitotic spindle

Journal of Cell Biology

Volumn 137, Issue 4, 1997, Pages 881-890

  Saunders, Robert D C ^a   Do Carmo Avides, Maria ^a   Howard, Thomas ^a   Gonzalez, Cayetano ^a,b   Glover, David M ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN BINDING PROTEIN; CALMODULIN BINDING PROTEIN; GENE PRODUCT; MICROTUBULE ASSOCIATED PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN KINASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CENTROSOME; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; EMBRYO; FEMALE; GENE MUTATION; IMMUNOFLUORESCENCE; MALE; MICROTUBULE ASSEMBLY; MITOSIS SPINDLE; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN PHOSPHORYLATION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BINDING SITES; CALMODULIN-BINDING PROTEINS; CLONING, MOLECULAR; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; GENES, INSECT; INSECT PROTEINS; MICROFILAMENT PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; MITOTIC SPINDLE APPARATUS; MOLECULAR SEQUENCE DATA;

EID: 15444361369     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.137.4.881     Document Type: Article

References (57)

1. Adams, A.E.M., W.Y. Shen, C.S. Lin, J. Leavitt, and P. Matsudaira. 1995. Isoform specific complementation of the yeast SAC6 null mutation by human fimbrin. Mol. Cell. Biol. 15:69-75.
2. Andersen, S.S.L., B. Buendia, J.E. Dominguez, A. Sawyer, and E. Karsenti. 1995. Effects on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus laevis eggs and dividing cells. J. Cell Biol. 127:1289-1299.
3. Baudier, J., J.C. Deloulme, A. Van Dorsselaer, D. Black, and H.W.D. Matthes. 1991. Purification and characterization of a brain-specific protein kinase C substrate, neurogranin (p17). J. Biol. Chem. 266:229-237.
4. Biggins, S., and M.D. Rose. 1994. Direct interaction between yeast spindle pole body component KAR1p is required for cdc31p localization to the spindle pole body. J. Cell Biol. 163:162-174.
5. Blanchard, A., V. Ohanian, and D. Critchley. 1989. The structure and function of alpha-actin. J. Muscle Res. Cell Motil. 10:280-289.
6. Bresnick, A.R., V. Warren, and J. Condelis. 1990. Identification of a short sequence essential for actin binding by Dictyostelium ABP-120. J. Biol. Chem. 265:9236-9240.
7. Brown, N.H., and F.C. Kafatos. 1988. Functional cDNA libraries from Drosophila embryos. J. Mol. Biol. 203:425-437.
8. Bulinski, J.C., and G.G. Borisy. 1980. Microtubule-associated proteins from cultured HeLa cells. J. Biol. Chem. 255:11570-11576.
9. Carmena, M., C. Gonzalez, J. Casai, and P. Ripoll. 1991. Dosage dependence of maternal contribution to somatic cell division in Drosophila melanogaster. Development (Camb.). 113:1357-1364.
10. Casal, J., C. Gonzalez, F. Wandosell, J. Avila, and P. Ripoll. 1990. Abnormal meiotic spindles cause a cascade of defects during spermatogenesis in asp males of Drosophila. Development (Camb.). 108:251-260.
11. Chapman, E.R., D. Au, K.A. Alexander, T.A. Nicolson, and D.R. Storm. 1991. Characterization of the calmodulin binding domain of neuromodulin. J. Biol. Chem. 266:207-213.
12. Cheney, R.E., and M.S. Mooseker. 1992. Unconventional myosins. Curr. Opin. Cell Biol. 4:27-35.
13. de Arruda, M.V., S. Watson, C.S. Lin, J. Leavitt, and P. Matsudaira. 1990. Fimbrin is a homolog of the cytoplasmic phosphoprotein plasmin and has domains homologous with calmodulin and actin gelation proteins. J. Cell Biol. 111:1069-1079.
14. Debrabander, M., J.C. Bulinski, G. Geuens, J. Demey, and G.G. Borisy. 1981. Immunoelectron microscopic localization of the 210,000-mol microtubule-associated protein in cultured cells of primate. J. Cell Biol. 91:438-445.
15. Fellous, A., V. Prasad, R. Ohayon, M.A. Jordan, and R.F. Luduena. 1994. Removal of the projection domain of microtubule-associated protein 2 alters its interaction with tubulin. J. Protein Chem. 13:381-391.
16. Finelli, L., T. Xie, C.A. Bossie, R.K. Blackman, and R.W. Padgett. 1995. The tolkin gene is a tolloid BMP-1 homolog that is essential for Drosophila development. Genetics. 141:271-281.
17. Geiser, J.R., H.A. Sundberg, B.H. Chang, E.G.D. Muller, and T.N. Davis. 1993. The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 7913-7924.
18. Goldstein, L.S.B., R.A. Laymon, and J.R. McIntosh. 1986. A microtubule-associated protein in Drosophila melanogaster: identification, characterization, and isolation of coding sequences. J. Cell Biol. 102:2076-2087.
19. Gonzalez, C., and D.M. Glover. 1993. Techniques for studying mitosis in Drosophila. In The Cell Cycle: A Practical Approach. P. Fantes and R. Brookes, editors. Oxford University Press, Oxford, UK. pp 163-168.
20. Gonzalez, C., I. Molina, J. Casal, and P. Ripoll. 1989. Gross genetic dissection and interaction of the chromosomal region 95E;96F of Drosophila melanogaster. Genetics. 123:371-377.
21. Gonzalez, C., R.D.C. Saunders, J. Casal, I. Molina, M. Carmena, P. Ripoll, and D.M. Glover. 1990. Mutations at the asp locus of Drosophila lead to multiple free centrosomes in syncytial embryos but restrict centrosome duplication in larval neuroblasts. J. Cell Sci. 96:605-616.
22. Harlow, E., and D. Lane. 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
23. Itoh, T.J., and H. Hotani. 1994. Microtubule-stabilizing activity of microtubule-associated proteins (MAPs) is due to increase in frequency of rescue in dynamic instability: shortening length decreases with binding of MAPs onto microtubules. Cell Struct. Func. 19:279-290.
24. Kilmartin, J.V., B. Wright, and C. Milstein. 1982. Rat Monoclonal anti tubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582.
25. Kilmartin, J.V., S.L. Dyos, D. Kershaw, and J.T. Finch. 1993. A spacer protein in the Saccharomyces cerevisiae spindle pole body whose transcript is cell cycle regulated. J. Cell Biol. 123:1175-1184.
26. Kowalski, R.J., and R.C. Williams. 1993. Microtubule-associated protein 2 alters the dynamic properties of microtubule assembly and disassembly. J. Biol. Chem. 268:9847-9855.
27. Leppard, K., N. Totty, M. Waterfield, E. Harlow, J. Jenkins, and L. Crawford. 1983. Purification and partial amino acid sequence of the cellular tumour antigen, p53, from mouse SV40 transformed cells. EMBO (Eur. Mol. Biol. Organ.) J. 2:1993-1999.
28. Lopez, L.A., and M.P. Sheetz. 1995. A microtubule-associated protein (MAP2) kinase restores microtubule motility in embryonic brain. J. Biol. Chem. 270: 12511-12517.
29. Loveland, K.L., T.M. Hayes, A. Meinhardt, K.S. Zlatic, M. Parvinen, D.M. Dekretser, and J.R. McFarlane. 1996. Microtubule-associated protein-2 in the rat testis: a novel site of expression. Biol. Reprod. 54:896-904.
30. Matsudaira, P. 1991. Modular organization of actin cross-linking proteins. Trends Biochem. Sci. 16:87-92.
31. Morin, M., A. Abrieu, T. Lorca, F. Martin, and M. Doree. 1994. The proteolysis-dependent metaphase to anaphase transition: calcium/calmodulin-dependent protein kinase II mediates onset of anaphase in extracts prepared from unfertilised Xenopus eggs. EMBO (Eur. Mol. Biol. Organ.) J. 13:4343-4352.
32. Murray, N.E. 1983. Phage Lambda and Molecular Cloning. In Lambda II. R.W. Hendrix, J.W. Roberts, F.W. Stahl, and R.A. Weisberg, editors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. pp 395-432.
33. Neel, V.A., and M.W. Young. 1994. igloo, a GAP-43-related gene expressed in the developing nervous system of Drosophila. Development (Camb.). 120: 2235-2243.
34. Noegel, A., W. Witke, and M. Schleicher. 1987. Calcium sensitive nonmuscle alpha-actinin contains EF-hand structures and highly conserved regions. FEBS (Fed. Eur. Biochem. Soc.) Lett. 221:391-396.
35. cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes. EMBO (Eur. Mol. Biol. Organ.) J. 11:1763-1772.
36. cdc2/cyclin B complex with microtubules in starfish oocytes. J. Cell Sci. 105:873-881.
37. cdc2kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. J. Cell Biol. 128:849-362.
38. Rappaport, R. 1986a. Simple methods and devices for handling echinoderm eggs. Methods Cell Biol. 27:345-358.
39. Rappaport, R. 1986b. Mitotic apparatus surface interaction and cell division, Development (Camb.). 9:263-277.
40. Ripoll, P., S. Pimpinelli, M.M. Valdivia, and J. Avila. 1985. A cell division mutant of Drosophila with a functionally abnormal spindle. Cell. 41:907-912.
41. Salisbury, J.L. 1995. Centrin, centrosomes, and mitotic spindle poles. Curr. Opin. Cell Biol. 7:39-45.
42. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Second edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
43. Saunders, R.D.C. 1994. In situ hybridization to Drosophila polytene chromosomes. Methods Mol. Biol. 33:183-191.
44. Saunders, R.D.C., D.M. Glover, M. Ashburner, I. Sidén-Kiamos, C. Louis, M. Monastirioti, C. Savakis, and F. Kafatos. 1989. PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning. Nucleic Acids Res. 17:9027-9037.
45. Scalenghe, F., E. Turco, J.E. Edström, V. Pirrotta, and M. Melli. 1985. Microdissection and cloning of DNA from specific regions of Drosophila melanogaster polytene chromosomes. Chromosoma (Berl.). 82:205-216.
46. Shimell, M.J., E.L. Ferguson, S.R. Childs, and M.B. O'Connor. 1991. The Drosophila dorsal-ventral patterning gene tolloid is related to human bone morphogenetic protein 1. Cell. 67:469-481.
47. Sidén-Kiamos, I., R.D.C. Saunders, L. Spanos, T. Majerus, J. Treanear, C. Savakis, C. Louis, D.M. Glover, M. Ashburner, and F.C. Kafatos. 1990. Towards a physical map of the Drosophila melanogaster genome: mapping of cosmid clones within defined genomic divisions. Nucleic Acids Res. 18:6261-6270.
48. Spang, A., I. Courtney, U. Fackler, M. Matzner, and E. Schiebel. 1993. The calcium binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body. J. Cell Biol. 123:405-416.
49. Spang, A., I. Courtney, K. Grein, M. Matzner, and E. Schiebel. 1995. The cdc31p binding protein kar1p is a component of the half bridge of the yeast spindle pole body. J. Cell Biol. 128:863-877.
50. Speek, M., J.W. Raff, K. Harrison-Lavoie, P.F.R. Little, and D.M. Glover. 1988. Smart2, a cosmid vector with a phage lambda origin for both systematic chromosome walking and P-element mediated gene transfer in Drosophila. Gene (Amst.). 64:173-177.
51. Stemple, D.L., S.C. Sweet, M.J. Welsh, and J.R. McIntosh. 1988. Dynamics of a fluorescent calmodulin analog in the mammalian mitotic spindle at metaphase. Cell Motil. Cytoskeleton. 9:231-242.
52. Stirling, D.A., K.A. Welch, and M.J.R. Stark. 1994. Interaction with calmodulin is required for the function of spc110p, an essential component of the yeast spindle pole body. EMBO (Eur. Mol. Biol. Organ.) J. 18:4329-4342.
53. Stirling, D.A., T.F. Rayner, A.R. Prescott, and M.J.R. Stark. 1996. Mutations which block the binding of calmodulin to spc110 cause multiple mitotic defects. J. Cell Sci. 109:1297-1310.
54. Sundberg, H.A., L. Goetsch, B. Byers, and T.N. Davis. 1996. Role of calmodulin and spe110p interaction in the proper assembly of spindle pole body components. J. Cell Biol. 133:111-124.
55. Umeyama, T., S. Okabe, Y. Kanai, and N. Hirokawa. 1993. Dynamics of microtubules bundled by microtubule-associated protein 2C (MAP2C). J. Cell Biol. 120:451-465.
56. Vallen, E.A., W. Ho, M. Winey, and M.D. Rose. 1994. Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae. Genetics. 137:407-422.
57. Yoshida, T., T. Toda, and M. Yanagida. 1994. A calcineurin-like gene PPB1(+) in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning. J. Cell Sci. 107:1725-1735.