The autolytic activity of the recombinant amidase of Staphylococcus saprophyticus is inhibited by its own recombinant GW repeats

FEMS Microbiology Letters

Volumn 227, Issue 1, 2003, Pages 47-51

  Hell, Wolfgang ^a,b   Reichl, Sylvia ^a   Anders, Agnes ^a   Gatermann, Sören ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b Landesuntersuchungsamt   (Germany)

Author keywords

Amidase; Bacterial autolysin; GW repeat; Staphylococcus saprophyticus; Turbidimetric assay

Indexed keywords

ADHESIN; AMIDASE; AUTOLYSIN; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOLYSIS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE REPEAT; PRIORITY JOURNAL; PROTEIN EXPRESSION; STAPHYLOCOCCUS SAPROPHYTICUS; TURBIDIMETRY; BACTERIUM ADHERENCE; CHEMISTRY; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; METABOLISM; PHYSIOLOGY; STAPHYLOCOCCUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STAPHYLOCOCCUS SAPROPHYTICUS;

AMIDOHYDROLASES; AUTOLYSIS; BACTERIAL ADHESION; RECOMBINANT PROTEINS; REPETITIVE SEQUENCES, NUCLEIC ACID; STAPHYLOCOCCUS;

EID: 1542753856     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00647-5     Document Type: Article

References (21)

1. Mendelson, N.H. (1982) Dynamics of Bacillus subtilis helical macrofiber morphogenesis: writhing, folding, close packing, and contraction. J. Bacteriol. 151, 438-449.
2. Hell, W., Meyer, H.G. and Gatermann, S.G. (1998) Cloning of aas, a gene encoding a Staphylococcus saprophyticus surface protein with adhesive and autolytic properties. Mol. Microbiol. 29, 871-881.
3. Heilmann, C., Hussain, M., Peters, G. and Gotz, F. (1997) Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface. Mol. Microbiol. 24, 1013-1024.
4. Oshida, T., Sugai, M., Komatsuzawa, H., Hong, Y.M., Suginaka, H. and Tomasz, A. (1995) A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N- acetylglucosaminidase domain: cloning, sequence analysis, and characterization. Proc. Natl. Acad. Sci. USA 92, 285-289.
5. Allignet, J., Aubert, S., Dyke, K.G. and ElSolh, N. (2001) Staphylococcus caprae strains carry determinants known to be involved in pathogenicity : a gene encoding an autolysin-binding fibronectin and the ica operon involved in biofilm formation. Infect. Immun. 69, 712-718.
6. Gatermann, S. and Meyer, H.G. (1994) Staphylococcus saprophyticus hemagglutinin binds fibronectin. infect. Immun. 62, 4556-4563.
7. Meyer, H.G., Muthing, J. and Gatermann, S.G. (1997) The hemagglutinin of Staphylococcus saprophyticus binds to a protein receptor on sheep erythrocytes. Med. Microbiol. Immunol. (Berl.) 186, 37-43.
8. Meyer, H.G., Wengler-Becker, U. and Gatermann, S.G. (1996) The hemagglutinin of Staphylococcus saprophyticus is a major adhesin for uroepithelial cells. Infect. Immun. 64, 3893-3896.
9. Braun, L., Dramsi, S., Dehoux, P., Bierne, H., Lindahl, G. and Cossart, P. (1997) In1B: an invasion protein of Listeria monocytogenes with a novel type of surface association. Mol. Microbiol. 25, 285-294.
10. Makino, S., Yamamoto, K., Murakami, S., Shirahata, T., Uemura, K., Sawada, T., Wakamoto, H., Morita, H. and Morita, Y.c.t.M.H. (1998) Properties of repeat domain found in a novel protective antigen, SpaA, of Erysipelothrix rhusiopathiae. Microb. Pathog. 25, 101-109
11. [erratum appeared in Microb. Pathog. 25(6) (1998) 353].
12. Garcia, P., Garcia, J.L., Garcia, E., Sanchez-Puelles, J.M. and Lopez, R. (1990) Modular organization of the lytic enzymes of Streptococcus pneumoniae and its bacteriophages. Gene 86, 81-88.
13. Garcia, P., PazGonzalez, M., Garcia, E., Garcia, J.L. and Lopez, R. (1999) The molecular characterization of the first autolytic lysozyme of Streptococcus pneumoniae reveals evolutionary mobile domains. Mol. Microbiol. 33, 128-138.
14. Holtje, J.V. and Tomasz, A. (1975) Specific recognition of choline residues in the cell wall teichoic acid by the N-acetylmuramyl-L-alanine amidase of Pneumococcus. J. Biol. Chem. 250, 6072-6076.
15. Baba, T. and Schneewind, O. (1998) Targeting of muralytic enzymes to the cell division site of Gram-positive bacteria: repeat domains direct autolysin to the equatorial surface ring of Staphylococcus aureus. EMBO J. 17, 4639-4646.
16. Jonquieres, R., Bierne, H., Fiedler, F., Gounon, P. and Cossart, P. (1999) Interaction between the protein in1B of Listeria monocytogenes and lipoteichoic acid: a novel mechanism of protein association at the surface of Gram-positive bacteria. Mol. Microbiol. 34, 902-914.
17. Milohanic, E., Jonquieres, R., Cossart, P., Berche, P. and Gaillard, J.L. (2001) The autolysin Ami contributes to the adhesion of Listeria monocytogenes to eukaryotic cells via its cell wall anchor. Mol. Microbiol. 39, 1212-1224.
18. Sambrook, J.F.E.F.a.M.T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Gatermann, S., Meyer, H.G. and Wanner, G. (1992) Staphylococcus saprophyticus hemagglutinin is a 160-kilodalton surface polypeptide. Infect. Immun. 60, 4127-4132.
20. Thumm, G. and Gotz, F. (1997) Studies on prolysostaphin processing and characterization of the lysostaphin immunity factor (Lif) of Staphylococcus simulans biovar staphylolyticus. Mol. Microbiol. 23, 1251-1265.
21. Takano, M., Oshida, T., Yasojima, A., Yamada, M., Okagaki, C., Sugai, M., Suginaka, H. and Matsushita, T. (2000) Modification of autolysis by synthetic peptides derived from the presumptive binding domain of Staphylococcus aureus autolysin. Microbiol. Immunol. 44, 463-472.