Rapid Detection of Klebsiella pneumoniae from Blood Culture Bottles by Real-Time PCR

Journal of Clinical Microbiology

Volumn 42, Issue 3, 2004, Pages 1337-1340

  Kurupati, Prathiba ^a   Chow, Carol ^b   Kumarasinghe, Gamini ^b   Poh, Chit Laa ^a,c  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY HOSPITAL   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM DETECTION; BLOOD CULTURE; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CROSS REACTION; DNA HYBRIDIZATION; DOUBLE BLIND PROCEDURE; HUMAN; KLEBSIELLA PNEUMONIAE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VALIDATION PROCESS;

BLOOD; COMPUTER SYSTEMS; CULTURE MEDIA; DNA PRIMERS; DNA, RIBOSOMAL; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; KLEBSIELLA PNEUMONIAE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE;

BACTERIA (MICROORGANISMS); KLEBSIELLA; KLEBSIELLA PNEUMONIAE; NEGIBACTERIA;

EID: 1542723489     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.3.1337-1340.2004     Document Type: Article

References (17)

1. Arlet, G., M. J. Sanson-Le Pors, M. Rouveau, G. Fournier, O. Marie, B. Schlemmer, and A. Philippon. 1990. Outbreak of nosocomial infection due to Klebsiella pneumoniae producing SHV-4 β-lactamase. Eur. J. Clin. Microbiol. Infect. Dis. 9:797-803.
2. Bellin, T., M. Pulz, A. Matussek, H. G. Hempen, and F. Gunzer. 2001, Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes. J. Clin. Microbiol. 39:370-374.
3. Bingen, E. H., P. Desjardins, G. Arlet, F. Bourgeois, P. Marianikurkdjian, N. Y. Lambertzechovsky, E. Denamur, A. Philippon, and J. Elion. 1993. Molecular epidemiology of plasmid spread among extended broad-spectrum β-lactamase-producing Klebsiella pneumoniae isolates in a pediatric hospital. J. Clin. Microbiol. 31:179-184.
4. Coovadia, Y. M., A. P. Johnson, R. H. Bhana, G. R. Hutchinson, R. C. George, and I. E. Hafferjee. 1992. Multiresistant Klebsiella pneumoniae in a neonatal nursery: the importance of maintenance of infection control policies and procedures in the prevention of outbreaks. J. Hosp. Infect. 22:197-205.
5. Corless, C. E., M. Guiver, R. Borrow, V. Edwards-Jones, A. J. Fox, and E. B. Kaczmarski. 2001. Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J. Clin. Microbiol. 39:1553-1558.
6. Cunney, R. J., E. B. McNamara, N. Alansari, B. Loo, and E. G. Smyth. 1997. The impact of blood culture reporting and clinical liaison on the empiric treatment of bacteraemia. J. Clin. Pathol. 50:1010-1012.
7. De Champs, C., D. Sirot, C. Chanal, M. C. Poupart, M. P. Dumas, and J. Sirot. 1991. Concomitant dissemination of three extended-spectrum β-lactamases among different Enterobacteriaceae isolated in a French hospital. J. Antimicrob. Chemother. 27:441-457.
8. Jacoby, G. A. 1996. Antimicrobial-resistant pathogens in the 1990s. Annu. Rev. Med. 47:169-179.
9. Jamulitrat, S., U. Meknavin, and S. Thongpiyapoon. 1994. Factors affecting mortality outcome and risk of developing nosocomial bloodstream infection. Infect. Control Hosp. Epidemiol. 15:163-170.
10. Johnson, A. P., M. J. Weinren, B. Ayling-Smith, S. K. Du Bois, S. G. B. Amyes, and R. C. George. 1992. Outbreak of infection in two U.K. hospitals caused by a strain of Klebsiella pneumoniae resistant to cefotaxime and ceftazidime. J. Hosp. Infect. 20:97-103.
11. Lebovici, L. S., I. Shraga, M. Drucker, H. Konigsberger, Z. Samra, and S. D. Pitlik. 1998. The benefit of appropriate empirical antibiotic treatment in patients with bloodstream infection. J. Intern. Med. 244:379-386.
12. Logan, J. M. J., K. J. Edwards, N. A. Saunders, and J. Stanley. 2001. Rapid identification of Campylobacter spp. by melting peak analysis of biprobes in real-time PCR. J. Clin. Microbiol. 39:2227-2232.
13. Nouvellon, M., J. L. Pons, D. Sirot, M. L. Combe, and J. F. Lemeland. 1994. Clonal outbreaks of extended-spectrum β-lactamase-producing strains of Klebsiella pneumoniae demonstrated by antibiotic susceptibility testing, β-lactamase typing, and multilocus enzyme electrophoresis. J. Clin. Microbiol. 32:2625-2627.
14. Pedersen, G., H. C. Schonheyder, and H. T. Sorensen. 1997. Antibiotic therapy and outcome of monomicrobial gram-negative bacteremia: a 3-year population-based study. Scand. J. Infect. Dis. 29:601-606.
15. Rice, L. B., S. H. Willey, G. A. Papanicolaou, A. A. Medeiros, G. M. Eliopoulos, R. C. Moellering, Jr., and G. A. Jacoby. 1990. Outbreak of ceftazidime resistance caused by extended-spectrum β-lactamases at a Massachusetts chronic-care facility. Antimicrob. Agents Chemother. 34:2193-2199.
16. Skeidsvoll, J., and P. M. Ueland. 1995. Analysis of double-stranded DNA by capillary electrophoresis with laser-induced fluorescence detection using the monomeric dye SYBR Green I. Anal. Biochem. 231:359-365.
17. Tyagi, S., and F. R. Kramer. 1996. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14:303-308.