Multiplex PCR Assay for Direct Identification of Group B Streptococcal Alpha-Protein-Like Protein Genes

Journal of Clinical Microbiology

Volumn 42, Issue 3, 2004, Pages 1326-1329

  Creti, Roberta ^a   Fabretti, Francesca ^a   Orefici, Graziella ^a   Von Hunolstein, Christina ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA C PROTEIN; ALPHA PROTEIN LIKE PROTEIN; BACTERIAL PROTEIN; EPSILON PROTEIN; GENE PRODUCT; MEMBRANE PROTEIN; PROTEIN ALP2; PROTEIN ALP3; PROTEIN ALP4; PROTEIN RIB; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; BACTERIAL GENE; EPIDEMIOLOGICAL DATA; GENE IDENTIFICATION; GENETIC ANALYSIS; MOLECULAR SIZE; MOLECULAR TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTOCOCCUS AGALACTIAE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; DNA PRIMERS; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; STREPTOCOCCUS AGALACTIAE;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS SP. 'GROUP B';

EID: 1542723488     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.3.1326-1329.2004     Document Type: Article

References (20)

1. Baker, C. J., and M. S. Edwards. 2003. Group B streptococcal conjugate vaccines. Arch. Dis. Child. 88:375-378.
2. Bolduc, G. R., M. J. Baron, C. Gravekamp, C. S. Lachenauer, and L. C. Madoff. 2002. The alpha C protein mediates internalization of group B streptococcus within human cervical epithelial cells. Cell Microbiol. 4:751-758.
3. Borchardt, S. M., B. Foxman, D. Chaffin, C. Rubens, P. A. Tallman, S. D. Manning, C. J. Baker, and C. F. Marrs. 2003. Molecular serotyping of group B streptococci (GBS) by PCR and DNA dot blot hybridization. Ann. Epidemiol. 13:581.
4. Creti, R., J. L. Michel, and G. Orefici. 2000. Genetic variability of the locus encoding alpha-C like proteins in Streptococcus agalactiae, p. 397-399. In D. R. Martin and John Tagg (ed.), Streptococci and streptococcal diseases: entering the new millenium. Proceedings of the XIV Lancefield International Symposium on Streptococci and Streptococcal Diseases. Securacopy, Porirua, New Zealand.
5. Ferrieri, P. 1988. Surface-localized protein antigens of group B streptococci. Rev. Infect. Dis. 10(Suppl. 2):S363-S366.
6. Gravekamp, C., D. L. Kasper, L. C. Paoletti, and L. C. Madoff. 1999. Alpha C protein as a carrier for type III capsular polysaccharide and as protective protein in group B streptococcal vaccines. Infect. Immun. 67:2491-2496.
7. Heden, L. O., E. Fritz, and G. Lindahl. 1991. Molecular characterization of an IgA receptor from group B streptococci: sequence of the gene, identification of a proline-rich region with unique structure and isolation of N-terminal fragments with IgA-binding capacity. Eur. J. Immunol. 21:1481-1490.
8. Jerlstrom, P. G., G. S. Chhatwal, and K. N. Timmis. 1991. The IgA-binding beta antigen of the C protein complex of group B streptococci: sequence determination of its gene and detection of two binding regions. Mol. Microbiol. 5:843-849.
9. Kong, F., S. Gowan, D. Martin, G. James, and G. L. Gilbert. 2002. Serotype identification of group B streptococci by PCR and sequencing. J. Clin. Microbiol. 40:216-226.
10. Kong, F., S. Gowan, D. Martin, G. James, and G. L. Gilbert. 2002. Molecular profiles of group B streptococcal surface protein antigen genes: relationship to molecular serotypes. J. Clin. Microbiol. 40:620-626.
11. Lachenauer, C. S., and L. C. Madoff. 1996. A protective surface protein from type V group B streptococci shares N-terminal sequence homology with the alpha C protein. Infect. Immun. 64:4255-4260.
12. Lachenauer, C. S., R. Creti, J. L. Michel, and L. C. Madoff. 2000. Mosaicism in the alpha-like protein genes of group B streptococci. Proc. Natl. Acad. Sci. USA 97:9630-9635.
13. Larsson, C., M. Stalhammar-Carlemalm, and G. Lindahl. 1996. Experimental vaccination against group B streptococcus, an encapsulated bacterium, with highly purified preparations of cell surface proteins Rib and α. Infect. Immun. 64:3518-3523.
14. Madoff, L. C., J. L. Michel, E. W. Gong, D. E. Kling, and D. L. Kasper. 1996. Group B streptococci escape host immunity by deletion of tandem repeat elements of the alpha C protein. Proc. Natl. Acad. Sci. USA 93:4131-4136.
15. Michel, J. L., L. C. Madoff, D. E. Kling, D. L. Kasper, and F. M. Ausubel. 1991. C proteins of group B streptococci, p. 214-218. In G. M. Dunny, P. P. Cleary, and L. L. McKay (ed.), Genetics and molecular biology of streptococci, lactococci, and enterococci. American Society for Microbiology, Washington, D.C.
16. Michel, J. L., L. C. Madoff, K. Olson, D. E. Kling, D. L. Kasper, and F. M. Ausubel. 1992. Large, identical, tandem repeating units in the C protein alpha antigen gene, bca, of group B streptococci. Proc. Natl. Acad. Sci. USA 89:10060-10064.
17. Paoletti, L. C., and D. L. Kasper. 2003. Glycoconjugate vaccines to prevent group B streptococcal infections. Expert Opin. Biol. Ther. 3:975-984.
18. Schuchat, A. 1999. Group B streptococcus. Lancet 353:51-56.
19. Spelleberg, B. 2000. Pathogenesis of neonatal S. agalactiae infections. Microbes Infect. 2:1733-1742.
20. Wastfelt, M., M. Stalhammar-Carlemalm, A. M. Delisse, T. Cabezon, and G. Lindhal. 1996. Identification of a family of streptococcal surface proteins with extremely repetitive structure. J. Biol: Chem. 271:18892-18897.