Probing essential residues for cellular uptake with a cationic nuclear localization signal sequence

Journal of Peptide Research

Volumn 63, Issue 2, 2004, Pages 155-160

  Ragin, A D ^a   Chmielewski, J ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Alanine scan; Cationic peptide; Cellular delivery

Indexed keywords

ALANINE; CATION; DNA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PEPTIDE; TRANSCRIPTION FACTOR;

ARTICLE; CELL ACTIVITY; CELL MEMBRANE; ELECTRICITY; EVALUATION; HYDROPHILICITY; HYDROPHOBICITY; MOLECULAR INTERACTION; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; STATISTICAL SIGNIFICANCE;

ACTIVE TRANSPORT, CELL NUCLEUS; ALANINE; CELL LINE; HUMANS; MICROSCOPY, FLUORESCENCE; MUTATION; NF-KAPPA B; NUCLEAR LOCALIZATION SIGNALS; PROTEIN TRANSPORT;

EID: 1542721097     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-3011.2003.00115.x     Document Type: Article

References (21)

1. Schwarze, S.R. & Dowdy, S.F. (2000) In vivo protein transduction: intracellular delivery of biologically active proteins, compounds and DNA. Trends. Pharm. Sci. 11, 45-48.
2. Hu, W.S. & Pathak, V.K. (2000) Design of retroviral vectors and helper cells for gene therapy. Pharmacol. Rev. 51, 493-511.
3. Smith, A.E. (1995) Viral vectors in gene therapy. Annu. Rev. Microbiol. 49, 807-838.
4. Chesnoy, S. & Huang, L. (2000) Structure and function of lipid-DNA complexes for gene therapy. Annu. Rev. Biophys. Biomol. Struct. 19, 27-47.
5. Devender, S., Reza, K., Kawamura, K., LaCasse, E.C. & Gariepy, J. (1998) Penetration and intracellular routing of nucleus directed peptide based shuttles (loligomers) in eukaryotic cells. Biochemistry 37, 5798-5809.
6. Leonetti, J.P., Deglos, G. & Lebleu, B. (1990) Biological activity of oligonucleotide-poly-(L-lysine) conjugates. Bioconjug. Chem. 1, 149-153.
7. Fawell, S., Seery, J., Daikh, Y., Moore, C., Chen, L.L., Pepinsky, B. & Barsoum, J. (1994) Tat-mediated delivery of heterologous proteins into cells. Proc. Natl. Acad. Sci. USA 91, 664-668.
8. Derossi, D., Joliot, A.H., Chassaing, G. & Prochiantz, A. (1994) The third helix of the Antennapedia homeodomain translocates through biological membranes. J. Bio. Chem. 169, 10444-10450.
9. Schwarze, S.R., Hruska, K.A. & Dowdy, S.F. (2000) Protein transduction: unrestricted delivery into cells. Trends Cell Biol. 10, 290-296.
10. Derossi, D., Chassaing, G. & Prochiantz, A. (1998) Trojan peptides: the penetratin system for intracellular delivery. Trends Cell Biol 8, 84-87.
11. Eguchi, A., Akuta, T., Okuyama, H., Senda, T., Yokoi, H., Inokuchi, H., Fujita, S., Hayakawa, T., Takeda, K., Hasegawa, M. (2001) Protein transduction domain of HTV-1 Tat protein promotes efficient delivery of DNA into mammalian cells. J. Biol Chem. 176, 26204-26210.
12. Polyakov, V., Sharma, V., Dahlheimer, J., Pica, C., Luker, G. & Piwnica-Worms, D. (2001) Novel Tat-peptide chelates for direct transduction of technetium-99m and rhenium into human cells for imaging and radiotherapy. Bioconj. Chem. 11, 762-771.
13. Lewin, M., Calesso, N., Tung, C.H., Tang, X.W., Cory, D., Scaden, D.T. & Weissleder, R. (2000) Tat peptide derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat. Biotechnol. 18, 410-414.
14. Torchillin, V., Rammohan, R., Weissig, V. & Levchenko, T. (2001) Tat peptide on the surface of liposomes affords their efficient delivery even at low temperature and in the presence of metabolic inhibitors. Proc. Natl. Acad. Sci. USA 98, 8786-8791.
15. Schwarze, S.R., Ho, A., Vocero-Akbani, A. & Dowdy, S.F. (1999) In vivo protein transduction of a biologically active protein into the mouse. Science 185, 1569-1572.
16. Elliot, G. & O'Hare, P. (1997) Intracellular trafficking and protein delivery by a herpesvirus structural protein. Cell 88, 223-233.
17. Mitchell, D.J., Kim, D.T., Steinman, L.C., Fathman, C.G. & Rothbard, J.B. (2000) Polyarginine enters cells more efficiently than other polycationic homopolymers. J. Pept. Res. 55, 318-325.
18. Wender, P.A., Mitchell, D.J., Pattabiraman, K., Pelkey, E.T., Steinmau, L. & Rothbard, J.B. (2000) The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake. Peptoid molecular transporters. Proc. Natl. Acad. Sci. USA 97, 13003-13008.
19. Ragin, A.D., Morgan, R.A. & Chmielewski, J. (2002) Cellular import mediated by nuclear localization signal peptide sequences. Chemistry & Biology 8, 943-948.
20. Belrose, J.P., Covert, O., Derossi, D., Brunissen, A., Chassaing, G. & Prochiantz, A. (1996) Conformational and associative behaviors of the third helix of Anntennapedia homeodomain in membrane-mimetic environment. Eur. J. Biochem. 141, 372-386.
21. Drin, G., Mazel, M., Clair, P., Mathieu, D., Kaczorek, M. & Temsamani, J. (2001) Physico-chemical requirements for cellular uptake of pAntp peptide. Eur. J. Biochem. 168, 1304-1314.