Partial inverse PCR : A technique for cloning flanking sequences

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1046-1048

  Pang, Ka Ming ^a   Knecht, David A ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; RESTRICTION ENDONUCLEASE;

ARTICLE; BINDING AFFINITY; DNA FLANKING REGION; ENZYME ACTIVITY; GENE SEQUENCE; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PROTEIN BINDING; SARCODINA;

SARCODINA;

EID: 1542563726     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226bm07     Document Type: Article

References (5)

1. Hock, R.S. and J.S. Condeelis. 1987. Isolation of a 240-kilodalton actin-binding protein from Dictyostelium discoideum. J. Biol. Chem. 262:394-400.
2. Ochman, H., A.S. Gerber and D.L. Hartl. 1988. Genetic applications of inverse polymerase chain reaction. Genetics 120:621-623.
3. Shyamala, V. and G.F.-L. Ames. 1989. Genome walking by single-specific-primer polymerase chain reaction: SSP-PCR. Gene 84:1-8.
4. Triglia, T., M.G. Peterson and D.J. Kemp. 1988. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16:8186.
5. Wang, K., B.F. Koop and L. Hood. 1994. A simple method using T4 DNA polymerase to clone polymerase chain reaction products. BioTechniques 17:236-238.