Purification and Characterization of a Pineapple Crown Leaf Thiol Protease

Preparative Biochemistry and Biotechnology

Volumn 34, Issue 1, 2004, Pages 25-43

  Singh, L Rupachandra ^a   Devi, Th Premila ^b   Devi, S Kunjeshwori ^a  

^a MANIPUR UNIVERSITY   (India)

^b ICAR RESEARCH COMPLEX FOR NEH REGION   (India)

Author keywords

Ananas comosus (L.) Merr.; Immunoaffinity enzyme purification; Pineapple crown leaf; Pineapple crown leaf protease; Thiol protease

Indexed keywords

AZOCASEIN; CASEIN; CYSTEINE; CYSTEINE PROTEINASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; DRUG EFFECT; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; ISOLATION AND PURIFICATION; KINETICS; PINEAPPLE; PLANT LEAF; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANANAS; CASEINS; CHROMATOGRAPHY, AFFINITY; CYSTEINE; CYSTEINE ENDOPEPTIDASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; KINETICS; PLANT LEAVES; SUBSTRATE SPECIFICITY;

ANANAS COMOSUS;

EID: 1542378234     PISSN: 10826068     EISSN: None     Source Type: Journal    
DOI: 10.1081/PB-120027111     Document Type: Article

References (43)

1. Murachi, T.; Neurath, H. Fractionation and specificity studies on stem bromelain. J. Biol. Chem. 1960, 235, 99-107.
2. Dixon, M.; Webb, E.C. Enzymes; Academic Press: New York, 1979.
3. Rowan, A.D.; Buttle, D.J.; Barrett, A.J. Ananain: a novel cysteine proteinase found in pineapple stem. Arch. Biochem. Biophys. 1988, 267, 262-270.
4. Gray, C.J.; Boukouvalas, J.; Szawelski, R.J.; Wharton, C.W. Benzyloxycarbonylphenylalanylcitrulline p-nitroanilide as a substrate for papain and other plant cystaine proteinases. Biochem. J. 1984, 219, 325-328.
5. Grayen, M., Ed. Encyclopedia of Chemical Technology; John Wiley & Sons: New York, 1980; 9, 201.
6. Ota, S.; Fu, T.H.; Hirohata, R. Studies on bromelain II: its activation and fractionation. J. Biochem. (Tokyo) 1961, 49, 532-537.
7. El-Gharbawi, M.; Whitaker, J.R. Fractionation and partial characterization of the proteolytic enzymes of stem bromelain. Biochemistry 1963, 2, 476-481.
8. Feinstein, G.; Whitaker, J.R. On the molecular weights of the proteolytic enzymes of stem bromelain. Biochemistry 1964, 3, 1050-1054.
9. Scocca, J.; Lee, Y.N. The composition and structure of the carbohydrate of pineapple stem bromelain. J. Biol. Chem. 1969, 244, 4852-4863.
10. Heinicke, R.M.; Gortner, W.A. Econ. Botany 1957, 11, 225-234.
11. Yamada, F.; Takahashi, N.; Murachi, T. Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2. J. Biochem. (Tokyo) 1976, 79, 1223-1234.
12. Ota, S.; Muta, E.; Katahira, Y.; Okamoto, Y. Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains. J. Biochem. (Tokyo) 1985, 98, 219-228.
13. Arroyo-Reyna, A.; Hernandez-Arana, A.; Arreguin-Espinosa, R. Circular dichroism of stem bromelain: a third spectral class within the family of cysteine proteinases. Biochem. J. 1994, 300, 107-110.
14. Napper, A.D.; Bennett, S.P.; Borowski, M.; Holdridge, M.B.; Leonard, M.J.C.; Rogers, E.E.; Duan, Y.; Laursen, R.A.; Reinhold, B.; Shames, S.L. Purification and characterization of multiple forms of the pineapple-stem- derived cysteine proteinases ananain and comosain. Biochem. J. 1994, 301, 727-735.
15. Harach, T.; Eckert, K.; Schuze-Forster, K.; Nuck, R.; Grunow, D.; Maurer, H.R. Isolation and partial characterization of basic proteinsases from stem bromelain. J. Protein Chem. 1995, 14 (1), 41-52.
16. Harrach, T.; Eckert, K.; Maurer, H.R.; Machleidt, I.; Machleidt, W.; Nuck, R. Isolation and characterization of two forms of an acidic bromelain stem proteinase. J. Protein Chem. 1998, 17 (4), 351-361.
17. Ota, S. On a minor component of proteolytic enzymes contained in the pineapple fruit. J. Biochem. (Tokyo) 1966, 59, 463-468.
18. Ota, S.; Horie, K.; Hagino, F.; Hashimoto, C.; Date, H. Fractionation and some properties of the proteolytically active components of bromelains in the stem and the fruit of the pineapple plant. J. Biochem. (Tokyo) 1972, 71, 817-830.
19. Ota, S.; Moore, S.; Stein, W.H. Preparation and chemical properties of purified stem and fruit bromelains. Biochemistry 1964, 3, 180-185.
20. Rowan, A.D.; Buttle, D.J.; Barrett, A.J. The cysteine proteinases of the pineapple plant. Biochem. J. 1990, 266, 869-875.
21. Rowan, A.D.; Buttle, D.J. Pineapple cysteine endopeptidases. Meth. Enzymol. 1994, 244, 555-568.
22. Carter, C.E.; Marriage, H.; Goodenough, P.W. Mutagenesis and kinetic studies of a plant cysteine proteinase with an unusual arrangement of acidic amino acids in and around the active site. Biochemistry 2000, 39, 11005-11013.
23. Lopez, L.M.; Sequeiros, C.; Natalucci, C.L.; Brullo, A.; Maras, B.; Barra, D.; Caffini, N.O. Purification and characterization of macrodontain I, a cysteine peptidase from unripe fruits of Pseudananas macrodontes (Morr.) harms Bromeliaceae. Protein Expr. Purif. 2000, 18 (2), 133-140.
24. National Research Council. Veterinary medical care. In Guide for the Care and Use of Laboratory Animals; National Academy Press: Washington, DC, 1996; Chapter 3, 56-70.
25. Heinrikson, R.L.; Kezdy, F.J. Acidic cysteine protease inhibitors from pineapple stem. Meth. Enzymol. 1976, 45, 740-751.
26. Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951, 193, 265-275.
27. Dunbar, B.S.; Schwoebel, E.D. Preparation of polyclonal antibodies. Meth. Enzymol. 1990, 182, 663-670.
28. Wilchek, M.; Miron, T.; Kohn, J. Affinity chromatography. Meth. Enzymol. 1984, 104, 3-55.
29. Laemmli, U.K. Cleavage of stuctural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970, 227, 680-685.
30. Boschi-Muller, S.; Azza, S.; Sanglier-Cianferani, S.; Talfournier, F.; Dorsselear, A.V.; Branlant, G. A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli. J. Biol. Chem. 2000, 275 (46), 35908-35913.
31. Silverstein, R.M.; Kezdy, F.J. Characterization of the pineapple stem proteases (bromelains). Arch. Biochem. Biophys. 1975, 167, 678-686.
32. Kortt, A.A.; Hinds, J.A.; Zerner, B. Specificity and pH dependence of ficin-catalyzed hydrolyses. Comparisons with bromelain specificity. Biochemistry 1974, 13, 2029-2037.
33. Greenberg, D.M. Plant proteolytic enzymes. Meth. Enzymol. 1955, 2, 54-64.
34. Daley, L.S.; Vines, H.M. Pineapple (Ananas comosus L., Merr.) leaf proteinase. Plant Sci. Lett. 1978, 11, 59-67.
35. Ievleva, E.V.; Zimacheva, A.V.; Huan, P.H.; Nhan, V.H.; Mosolov, V.V. Proteinases from proliferous tops of pineapple fruits. Prikl. Biokhim. Microbiol. 1991, 27, 639-645.
36. Murachi, T.; Yasui, M.; Yasuda, Y. Purification and physical characterization of stem bromelain. Biochemistry 1964, 3, 48-55.
37. Murachi, T. Bromelain enzymes. Meth. Enzymol. 1970, 19, 273-284.
38. Murachi, T. Bromelain enzymes. Meth. Enzymol. 1976, 45, 475-485.
39. Ota, S.; Horie, K.; Hagino, F.; Hashimoto, C.; Date, H. J. Biochem. 1972, 72, 217-230.
40. Haq, S.K.; Rasheedi, S.; Khan, R.H. Characterization of a partially folded intermediate of stem bromelain at low pH. Eur. J. Biochem. 2002, 269, 47-52.
41. Ritonja, A.; Rowan, A.D.; Buttle, D.J.; Rawings, N.D.; Turk, V.; Barrett, A.J. Stem bromelain: amino acid sequence and implications for weak binding of cystatin. FEBS Lett. 1989, 247, 419-424.
42. Lee, K.L.; Albee, K.L.; Bernasconi, R.J.; Edmunds, T. Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. Biochem. J. 1997, 327, 199-202.
43. α- CBZ-L-lysine p-nitrophenyl ester and N-CBZ-glycine p-nitrophenyl ester as substrates. Anal. Biochem. 1974, 62, 478-484.