Peroxidase catalyzed formation of azine pigments - A convenient and sensitive method for the identification of human cells with positive myeloperoxidase reactivity

Bioorganic and Medicinal Chemistry Letters

Volumn 14, Issue 6, 2004, Pages 1509-1511

  Bodtke, Anja ^a   Pfeiffer, Wolf Diethard ^a   Ahrens, Norbert ^a   Langer, Peter ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Azine; Cell; Enzyme; Peroxidase; Pigment

Indexed keywords

1 NAPHTHOL; 1,4 NAPHTHOQUINONE DERIVATIVE; HORSERADISH PEROXIDASE; MYELOPEROXIDASE; PIGMENT; THIAZOLE DERIVATIVE;

ARTICLE; CATALYSIS; CATALYST; ENZYME ACTIVITY; HUMAN; HUMAN CELL; METHODOLOGY; OXIDATION;

CATALYSIS; HUMANS; LYMPHOCYTES; MONOCYTES; NAPHTHOQUINONES; PEROXIDASE; PIGMENTS, BIOLOGICAL; SENSITIVITY AND SPECIFICITY;

ARMORACIA RUSTICANA;

EID: 1542374746     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.01.001     Document Type: Article

References (21)

1. Dargel R. Entzündung. 1995;Ullstein Mosby, Berlin.
2. Johnson K.J., Varani J., Smolen J.E. Neutrophil activation and function in health and disease. Coffey R.G. Granulocyte Responses to Cytokines. 1992;1-46 Marcel Dekker Inc, New York.
3. Kölmel H.W. Liquorzytologie. 1978;Springer-Verlag, Berlin Heidelberg New York. p 10.
4. Luppa H. Grundlagen der Histochemie. 1977;Akad, Verlag Berlin. p 259.
5. Hennemann, H. H. Hämatologie; New York, 1991.
6. Begemann, H., Rastetter, J. Klinische Hämatologie, Thieme, Stuttgart, 1993; p 74.
7. Undritz E. Schweiz. med. WSchr. 95:1965;1479.
8. Lojda Z., Gossrau R., Schiebler T.H. Enzyme Histochemistry. 1979;Springer-Verlag.
9. For a recent application of the benzidine methodology, see: Tatzber F., Griebenow S., Wonisch W., Winkler R. Analyt. Biochem. 316:2003;147.
10. Lorentz K., Berndt W.R. Analyt. Biochem. 18:1967;58.
11. 4-pyrazolin-5-ones, see: Pfeiffer W.-D., Bulka E., Ahrens N. J. Prakt. Chem. 334:1992;365.
12. Enzyme catalyzed transformations play an important role in bioorganic chemistry: Enzyme Catalysis in Organic Synthesis; Drauz, K., Waldmann, H., eds. ; VCH: Weinheim, 1995.
13. Berglund G.I., Carlsson G.H., Smith A.T., Szöke H., Henriksen A., Hajdu J. Nature. 417:2002;463. and references cited therein.
14. Pfeiffer, W.-D. in Houben-Weyl, Methods of Organic Chemistry, Vol. E 9c, 483, Thieme Verlag, Stuttgart, 1997.
15. For related azines, see: Hünig S., Fritsch K.H. Liebigs Ann. Chem. 609:1957;143.
16. Hünig S. Angew. Chem. 70:1958;215.
17. 3OS (359.4): C 70.18, H 4.77, N 11.69. Found: C 69.90, H 5.16, N 11.88.
18. 2 (30%). To solution 2 (10 mL) were added solution 3 (15 μL) and solution 1 (10 mL) to give solution 4. The histo-cytologic material (30-50 μL) was fixed for 1-3 min by vapour-phase contact with formalin (35%), washed with water and dried in the air. Incubation of this material with solution 4 (1 mL) for 5-10 min and subsequent washing with water resulted in a positive peroxidase reaction which could be directly observed by microscopy (see Fig. 1). A quantification of the enzyme activity was not carried out.
19. Kaufmann, S. H. E., in: Gemsa, D., et al. Immunologie; Georg Thieme Verlag: Stuttgart-New York, 1991; p 150.
20. Sayk J. Ärzt. Wschr. 9:1954;1024.
21. see also: ref. 3, p 3.