Transgenic indica rice resistant to sap-sucking insects

Plant Biotechnology Journal

Volumn 1, Issue 3, 2003, Pages 231-240

  Nagadhara, D ^a   Ramesh, S ^a   Pasalu, I C ^b   Rao, Y Kondala ^b   Krishnaiah, N V ^b   Sarma, N P ^b   Bown, D P ^c   Gatehouse, J A ^c   Reddy, V D ^a   Rao, K V ^a  

^a OSMANIA UNIVERSITY   (India)

^b DIRECTORATE OF RICE RESEARCH   (India)

^c DURHAM UNIVERSITY   (United Kingdom)

Author keywords

Agrobacterium mediated transformation; Brown planthopper; Green leafhopper; Indica rice; Insect resistance; Snowdrop lectin

Indexed keywords

EID: 1542367700     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1046/j.1467-7652.2003.00022.x     Document Type: Article

References (37)

1. Aldemita, R.R. and Hodges, T.K. (1996) Agrobacterium tumefaciens– tumefaciens-mediated transformation of indica and japonica rice varieties. Planta, 199, 612–617.
2. Boulter, D., Edwards, G.A., Gatehouse, A.M.R., Gatehouse, J.A. and Hilder, V.A. (1990) Additive protective effects of different plant derived insect resistance genes in transgenic tobacco plants. Crop Protection, 9, 351–354.
3. Dong, J., Teng, W., Buchholz, W.G. and Hall, T.C. (1996) Agrobacterium-mediated transformation of Javanica rice. Mol. Breed. 2, 267–276.
4. Eisemann, C.H., Donaldson, R.A., Pearson, R.D., Cadagon, L.C., Vuocolo, T. and Pellam, R.L. (1994) Larvicidal action of lectins on Lucilia cuprina; mechanism of action. Entomol. Exp. Appl. 72, 1–11.
5. Estruch, J.J., Carozzi, N.B., Desai, N., Duck, N.B., Warren, G.W. and Koziel, M.G. (1997) Transgenic plants: An emerging approach to pest control. Nat. Biotechnol. 15, 137–141.pmid/9035137
6. Fitches, E., Woodhouse, S.D., Edwards, J.P. and Gatehouse, J.A. (2001) In vitro and in vivo binding of snowdrop lectin (Galanthus nivalis agglutinin; GNA) and jackbean (Canavalia ensiformis; Con A) lectins within tomato moth (Lacanobia oleraceae) larvae; mechanisms of insecticidal action. J. Insect Physiol. 47, 777–787.pmid/11356425
7. Hansen, G., Shillito, R.D. and Chilton, M.D. (1997) T-strand integration in maize protoplasts after co-delivery of a T-DNA substrate and virulence genes. Proc. Natl Acad. Sci. USA, 94, 11726–11730.pmid/9326678
8. Herdt, R.W. (1991) Research priorities for rice biotechnology. In: Rice Biotechnology (Khush, G.S. and Tenniessen, G.H., eds), pp. 19–54. Wallingford, UK: CAB International.
9. Hiei, Y., Ohta, S., Komari, T. and Kumashiro, T. (1994) Efficient transformation of rice (Oryza sativa) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant. J. 6, 271–282.pmid/7920717
10. Hilder, V.A., Gatehouse, A.M.R., Shreeman, S.E., Barker, R.F. and Boulter, D. (1987) A novel mechanism of insect resistance engineered into tobacco. Nature, 330, 160–163.
11. Jouanin, L., Bonade-Bottino, M., Girard, C., Morrot, G. and Giband, M. (1998) Transgenic plants for insect resistance. Plant Sci. 131, 1–11.
12. Kalode, M.B., Viswanathan, P.R.K. and Seshu, D.V. (1975) Standard test to characterize host plant resistance to brown planthopper in rice. Indian. J. Plant Prot. 3, 204–206.
13. Komari, T., Hiei, Y., Saito, Y., Murai, N. and Kumashiro, T. (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J. 10, 165–174.pmid/8758986
14. Koncz, C., Nineth, K., Redei, G.P. and Scell, J. (1994) Homologous recombination and gene silencing in plants. In: Homologous Recombination and Gene Silencing in Plants (Paszkouski, J., ed.), pp. 167–189. Dordrecht, The Netherlands: Kluwer Academic.
15. Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head of Bacteriophage T4. Nature, 227, 680–685.pmid/5432063
16. Lichtenstein, C. and Draper, J. (1985) Genetic engineering of plants. In: DNA Cloning, a Practical Approach (Glover, D.M., ed.), pp. 67–118. Washington DC: IRL Press.
17. Loc, N.T., Tinjuangjun, P., Gatehouse, A.M.R., Christou, P. and Gatehouse, J.A. (2002) Linear transgene constructs lacking vector backbone sequences generate transgenic rice plants which accumulate higher levels of proteins conferring insect resistance. Mol. Breed. 9, 231–244.
18. Lowry, O.H., Roseborough, N.J., Farr, A.L. and Randall, J.L. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.
19. Maqbool, S.B., Riazuddin, S., Loc, N.T., Gatehouse, A.M.R., Gatehouse, J.A. and Christou, P. (2001) Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Mol. Breed. 7, 85–93.
20. McCouch, S.R., Kochert, G., Yu, Z.H., Wang, Z.Y., Kush, G.S., Coffman, W.R. and Tanksley, S.D. (1988) Molecular mapping of rice chromosomes. Theor. Appl. Genet. 76, 815–829.
21. Mochida, O., Wahyu, A. and Surjani, T.K. (1979) Some Considerations on Screening Resistant Cultivars/lines of Rice Plant to the Brown Planthopper, Nilaparvata Lugens (Stål) (Hom., Delphacidae), pp. 1–9. Los Banos, Philippines: IRRI.
22. Murashige, T. and Skoog, F.A. (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
23. Pathak, P.K., Heinrichs, E.A. and Pathak, M.D. (1980) Development of Biotypes of Nilaparvata lugens (Stål), pp. 1–13. Los Banos, Philippines: IRRI.
24. Peferoen, M. (1992) Engineering of insect-resistant plants with Bacillus thuringiensis crystal protein genes. In: Plant Genetic Manipulation for Crops Protection (Gatehouse, A.M.R., Hilder, V.A. and Boulter, D., eds), pp. 135–153. Wallingford, UK: CAB International.
25. Powell, K.S., Gatehouse, A.M.R., Hilder, V.A. and Gatehouse, J.A. (1993) Antimetabolic effects of plant lectins and plant and fungal enzymes on nymphal stages of two important rice pests, Nilaparvatha lugens and Nephotettix cinciteps. Entomol. Exp. Appl. 66, 119–126.
26. Powell, K.S., Spence, J., Bharathi, M., Gatehouse, J.A. and Gatehouse, A.M.R. (1998) Immunohistochemical and developmental studies to elucidate the mechanism of action of the snowdrop lectin on rice brown planthopper, Nilaparvata lugens (Stål). J. Insect Physiol. 44, 529–539.
27. Pusztai, A.J. (1991) Plant Lectins. Cambridge, UK: Cambridge University Press.
28. Rao, K.V., Rathore, K.S., Hodges, T.K., Fu, X., Stoger, E., Sudhakar, D., Williams, S., Christou, P., Bharathi, M., Bown, D.P., Powell, K.S., Spence, J., Gatehouse, A.M.R. and Gatehouse, J.A. (1998) Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper. Plant J. 15, 469–477.pmid/9753773
29. Rathore, K.S., Chowdhury, V.K. and Hodges, T.K. (1993) Use of bar as a selectable marker gene for the production of herbicide resistant rice plants from protoplasts. Plant Mol. Biol. 21, 871–884.pmid/8467080
30. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
31. Saxena, R.C. and Khan, Z.R. (1989) Factors effecting resistance to rice varieties to planthopper and leafhopper pests. Agri. Zool. Rev. 3, 97–132.
32. Shi, Y., Wang, M.B., Powell, K.S., Van Damme, E., Hilder, V.A., Gatehouse, A.M.R., Boulter, D. and Gatehouse, J.A. (1994) Use of the rice sucrose synthase-1 promoter to direct phloem-specific expression of β-glucuronidase and snowdrop lectin genes in transgenic tobacco plants. J. Exp. Bot. 45, 623–631.
33. Sivamani, E., Huet, H., Shen, P., Ong, C.A., De Kochko, A., Fauquet, C. and Beachy, R.N. (1999) Rice plant (Oryza sativa L.) containing rice tungro spherical virus (RTSV) coat protein transgenes are resistant to virus infection. Mol. Breed. 5, 177–185.
34. Stoger, E., Williams, S., Christou, P., Down, R.E. and Gatehouse, J.A. (1999) Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae. Mol. Breed. 5, 65–73.
35. Towbin, H., Steahelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl Acad. Sci. USA, 76, 4350–4354.pmid/388439
36. Van Damme, E.J.M., Allen, A.K. and Peumans, W.J. (1987) Isolation and characterization of lectin with exclusive specificity towards mannose from snowdrop (Galanthus nivalis) bulbs. FEBS Lett. 215, 140–144.
37. Wu, A., Sun, X., Pang, Y. and Tang, K. (2002) Homozygous transgenic rice lines expressing GNA with enhanced resistance to the rice sap-sucking pest Laodelphax striatellus. Plant Breed. 121, 93–95.