The Amino-Terminal Region of Major Capsid Protein P3 Is Essential for Self-Assembly of Single-Shelled Core-Like Particles of Rice Dwarf Virus

Journal of Virology

Volumn 78, Issue 6, 2004, Pages 3145-3148

  Hagiwara, Kyoji ^a   Higashi, Takahiko ^a   Miyazaki, Naoyuki ^b   Naitow, Hisashi ^c   Cheng, R Holland ^e   Nakagawa, Atsushi ^b   Mizuno, Hiroshi ^d   Tsukihara, Tomitake ^b   Omura, Toshihiro ^a  

^a NATIONAL AGRICULTURAL RESEARCH CENTER   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c RIKEN HARIMA INSTITUTE   (Japan)

^d NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^e KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CAPSID PROTEIN; CAPSID PROTEIN P3; CORE PROTEIN; DOUBLE STRANDED RNA; POLYHEDRIN; RNA DIRECTED RNA POLYMERASE; UNCLASSIFIED DRUG; VIRUS DNA;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOGRAPHA CALIFORNICA; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; DELETION MUTANT; ELECTRON MICROSCOPY; LEPIDOPTERA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REOVIRUS; RICE DWARF VIRUS; VIRUS CORE; WESTERN BLOTTING;

ANIMALS; CAPSID PROTEINS; CELL LINE; DIMERIZATION; GENE DELETION; MODELS, MOLECULAR; NUCLEOPOLYHEDROVIRUS; ORYZA SATIVA; REOVIRIDAE; STRUCTURE-ACTIVITY RELATIONSHIP; VIRION; VIRUS ASSEMBLY;

EID: 1542347737     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.78.6.3145-3148.2004     Document Type: Article

References (9)

1. Grimes, J. M., J. N. Burroughs, P. Gouet, J. M. Diprose, R. Malby, S. Zientara, P. P. C. Mertens, and D. I. Stuart. 1998. The atomic structure of the bluetongue virus core. Nature 395:470-478.
2. Hagiwara, K., T. Higashi, K. Namba, T. Uehara-Ichiki, and T. Omura. 2003. Assembly of single-shelled cores and double-shelled virus-like particles after baculovirus expression of major structural proteins P3, P7 and P8 of Rice dwarf virus. J. Gen. Virol. 84:981-984.
3. Hewat, E. A., T. F. Booth, P. T. Loudon, and P. Roy. 1992. Three-dimensional reconstruction of baculovirus expressed bluetongue virus core-like particles by cryo-electron microscopy. Virology 189:10-20.
4. Kim, J., X. Zhang, V. E. Centonze, V. D. Bowman, S. Nobel, T. S. Baker, and M. L. Nibert. 2003. The hydrophilic amino-terminal arm of reovirus core shell protein XI is dispensable for particle assembly. J. Virol. 76:12211-12222.
5. Loudon, P. T., and P. Roy. 1991. Assembly of five bluetongue virus proteins expressed by recombinant baculoviruses: inclusion of the largest protein VPI in the core and virus-like particles. Virology 180:798-802.
6. Nakagawa, A., N. Miyazaki, J. Taka, H. Naitow, A. Ogawa, Z. Fujimoto, H. Mizuno, T. Higashi, Y. Watanabe, T. Omura, R. H. Cheng, and T. Tsukihara. 2003. The atomic structure of Rice dwarf virus reveals the self-assembly mechanism of component proteins. Structure 11:1227-1238.
7. Omura, T., and J. Yan. 1999. Role of outer capsid proteins in transmission of Phytoreovirus by insect vectors. Adv. Virus Res. 54:15-43.
8. Reinisch, K. M., M. L. Nibert, and S. C. Harrison. 2000. Structure of the reovirus core at 3.6 Å resolution. Nature 404:960-967.
9. Zhong, B., A. Kikuchi, Y. Moriyasu, T. Higashi, K. Hagiwara, and T. Omura. 2003. A minor outer capsid protein, P9, of Rice dwarf virus. Arch. Virol. 148:2275-2280.