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Volumn 53, Issue 1, 2004, Pages 109-113

Fluorescence Competition Assay for the Assessment of ATP Binding to an Isolated Domain of Na+,K+-ATPase

Author keywords

Competition; Dissociation constant; Enzyme ligand interaction; Fluorescence; TNP ATP

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ADENOSINE TRIPHOSPHATE;

EID: 1542346042     PISSN: 08628408     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (17)

References (14)
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  • 3
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    • Fluorescence properties of 2′ (or 3′ )-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate and its use in the study of binding to heavy meromyosin ATPase
    • HIRATSUKA T: Fluorescence properties of 2′ (or 3′ )-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate and its use in the study of binding to heavy meromyosin ATPase. Biochim Biophys Acta 453: 293-297, 1976
    • (1976) Biochim Biophys Acta , vol.453 , pp. 293-297
    • Hiratsuka, T.1
  • 4
    • 0015894660 scopus 로고
    • Preparation and properties of 2′ (or 3′ )-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate, an analog of adenosine triphosphate
    • HIRATSUKA T, UCHIDA K: Preparation and properties of 2′ (or 3′)-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate, an analog of adenosine triphosphate. Biochim Biophys Acta 320: 635-647, 1973
    • (1973) Biochim Biophys Acta , vol.320 , pp. 635-647
    • Hiratsuka, T.1    Uchida, K.2
  • 5
    • 0023432178 scopus 로고
    • An accurate method for determination of receptor-ligand and enzyme inhibitor dissociation constants from displacement curves
    • HOROVITZ A, LEVITZKI A: An accurate method for determination of receptor-ligand and enzyme inhibitor dissociation constants from displacement curves. Proc Natl Acad Sci USA 84: 6654-6658, 1987
    • (1987) Proc Natl Acad Sci USA , vol.84 , pp. 6654-6658
    • Horovitz, A.1    Levitzki, A.2
  • 7
    • 0032586721 scopus 로고    scopus 로고
    • 1 in a competitive enzyme-inhibition model: Comparisons among three methods of data analysis
    • 1 in a competitive enzyme-inhibition model: comparisons among three methods of data analysis. Drug Metab Dispos 27: 756-762, 1999
    • (1999) Drug Metab Dispos , vol.27 , pp. 756-762
    • Kakkar, T.1    Boxenbaum, H.2    Mayersohn, M.3
  • 8
    • 0029422355 scopus 로고
    • Na,K-adenosinetriphosphatase: The paradigm of a membrane transport protein
    • KOTYK A, AMLER E: Na,K-adenosinetriphosphatase: the paradigm of a membrane transport protein. Physiol Res 44: 261-274, 1995
    • (1995) Physiol Res , vol.44 , pp. 261-274
    • Kotyk, A.1    Amler, E.2
  • 12
    • 0019497930 scopus 로고
    • Characterization of 2′,3′ -O-(2,4,6-trinitrocyclohexadienylidine)adenosine 5′-triphosphate as a fluorescent probe of the ATP site of sodium and potassium transport adenosine triphosphatase
    • MOCZYDLOWSKI EG, FORTES PAG: Characterization of 2′,3′ -O-(2,4,6-trinitrocyclohexadienylidine)adenosine 5′-triphosphate as a fluorescent probe of the ATP site of sodium and potassium transport adenosine triphosphatase. J Biol Chem 256: 2346-2356, 1981
    • (1981) J Biol Chem , vol.256 , pp. 2346-2356
    • Moczydlowski, E.G.1    Fortes, P.A.G.2
  • 13
    • 0032975040 scopus 로고    scopus 로고
    • Catalytic activity of an isolated domain of Na,K-ATPase expressed in Escherichia coli
    • TRAN CM, FARLEY RA: Catalytic activity of an isolated domain of Na,K-ATPase expressed in Escherichia coli. Biophys J 77: 258-266, 1999
    • (1999) Biophys J , vol.77 , pp. 258-266
    • Tran, C.M.1    Farley, R.A.2
  • 14
    • 0028936571 scopus 로고
    • An exact mathematical expression for describing competitive binding of two different ligands to a protein molecule
    • WANG ZX: An exact mathematical expression for describing competitive binding of two different ligands to a protein molecule. FEBS Lett 360: 111-114, 1995
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    • Wang, Z.X.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.