Arabidopsis mutants in the C-S lyase of glucosinolate biosynthesis establish a critical role for indole-3-acetaldoxime in auxin homeostasis

Plant Journal

Volumn 37, Issue 5, 2004, Pages 770-777

  Mikkelsen, Michael Dalgaard ^b   Naur, Peter ^a   Halkier, Barbara Ann ^b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b NONE

Author keywords

Arabidopsis; Auxin; C S lyase; Glucosinolates; Indole 3 acetaldoxime; Superroot1

Indexed keywords

ACETIC ACID; BIOSYNTHESIS; ESCHERICHIA COLI; METABOLISM; METABOLITES; MUTAGENESIS;

HOMEOSTASIS; MUTANTS;

PLANT CELL CULTURE;

ARABIDOPSIS PROTEIN; CARBON SULFUR LYASE; CARBON-SULFUR LYASE; GLUCOSINOLATE; INDOLE 3 ACETALDOXIME; INDOLE DERIVATIVE; INDOLE-3-ACETALDOXIME; INDOLEACETIC ACID DERIVATIVE; LYASE; OXIME;

ARABIDOPSIS; ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MULTIGENE FAMILY; MUTATION; PHYSIOLOGY;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLUCOSINOLATES; INDOLEACETIC ACIDS; INDOLES; LYASES; MULTIGENE FAMILY; MUTATION; OXIMES;

ARABIDOPSIS; EMBRYOPHYTA; ESCHERICHIA COLI;

EID: 1542318946     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2004.02002.x     Document Type: Article

References (35)

1. Bak, S. and Feyereisen, R. (2001) The involvement of two P450 enzymes, CYP83B1 and CYP83A1, in auxin homeostasis and glucosinolate biosynthesis. Plant Physiol. 127, 108-118.
2. Bak, S., Olsen, C.E., Petersen, B.L., Møller, B.L. and Halkier, B.A. (1999) Metabolic engineering of p-hydroxybenzylglucosinolate in Arabidopsis by expression of the cyanogenic CYP79A1 from Sorghum bicolor. Plant J. 20, 663-670.
3. Bak, S., Tax, F.E., Feldmann, K.A., Galbralth, D.W. and Feyereisen, R. (2001) CYP83B1, a cytochrome P450 at the metabolic branch-point in auxin and indole glucosinolate biosynthesis in Arabidopsis. Plant Cell, 13, 101-111.
4. Barlier, I., Kowalczyk, M., Marchant, A., Ljung, K., Bhalerao, R., Bennett, M., Sandberg, G. and Bellini, C. (2000) The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450 CYP83B1, a modulator of auxin homeostasis. Proc. Natl. Acad. Sci. USA, 19, 14819-14824.
5. Bartel, B., LeClere, S., Magidin, M. and Zolman, B.K. (2001) Inputs to the active indole-3-acetic acid pool: de novo synthesis, conjugate hydrolysis, and indole-3-butyric acid β-oxidation. J. Plant Growth Regul. 20, 198-216.
6. Boerjan, W., Cervera, M.-T., Delarue, M., Beeckman, T., Dewitte, W., Bellini, C., Caboche, M., Onckelen, H.V., Montagu, M.V. and Inzé, D. (1995) superroot, a recessive mutation in Arabidopsis, confers auxin overproduction. Plant Cell, 7, 1405-1419.
7. Celenza, J.L., Grisafi, P.L. and Fink, G.R. (1995) A pathway for lateral root-formation in Arabidopsis thaliana. Genes Dev. 9, 2131-2142.
8. Cohen, J.D., Slovin, J.P. and Hendrickson, A.M. (2003) Two genetically discrete pathways convert tryptophan to auxin: more redundancy in auxin biosynthesis. Trends Plant Sci. 8, 197-199.
9. Delarue, M., Prinsen, E., Onckelen, H.V., Caboche, M. and Bellini, C. (1998) Sur2 mutations of Arabidopsis thaliana define a new locus involved in the control of auxin homeostasis. Plant J. 14, 603-611.
10. Giovanelli, J. and Mudd, S.H. (1971) Transsulfuration in higher plants: partial purification and properties of a β-cystathionase of spinach. Biochim. Biophys. Acta, 227, 654-670.
11. Gopalraj, M., Tseng, T.-S. and Olszewski, N. (1996)The Rooty gene of Arabidopsis encodes a protein with highest similarity to aminotransferases. Plant. Physiol. 111, S-114.
12. Griffiths, D.W., Deighton, N., Birch, A.N., Patrian, B., Baur, R. and Stadler, E. (2001) Identification of glucosinolates on the leaf surface of plants from the Cruciferae and other closely related species. Phytochemistry, 57, 693-700.
13. Halkler, B.A. (1999) Glucosinolates. In Naturally Occurring Glycosides (Ikan, R., ed.). New York: John Wiley & Sons Inc., pp. 193-223.
14. TYR expressed at high levels in Escherichia coli. Arch. Biochem. Biophys. 322, 369-377.
15. Hansen, C.H., Du, L., Naur, P., Olsen, C.E., Axelsen, C.B., Hick, A.J., Pickett, J.A. and Halkier, B.A. (2001) CYP83B1 is the oxime-metabolizing enzyme in the glucosinolate pathway in Arabidopsis. J. Biol. Chem. 276, 24790-24796.
16. Hull, A.K., Rekha, V. and Celenza, J.L. (2000) Arabidopsis cytochrome P450s that catalyze the first step of tryptophan-dependent indole-3-acetic acid biosynthesis. Proc. Natl. Acad. Sci. USA, 97, 2379-2384.
17. Jones, P.R., Manabe, T., Awazuhara, M. and Saito, K. (2003) A new member of plant CS-lyases. J. Biol. Chem. 278, 10291-10296.
18. King, J.J., Stimart, D.P., Fisher, R.H. and Bleecker, A.B. (1995) A mutation altering auxin homeostasis and plant morphology in Arabidopsis. Plant Cell, 7, 2023-2037.
19. Lang, T. and Kessler, D. (1999) Evidence for cysteine persulfide as reaction product of L-cyst(e)ine C-S-lyase (C-DES) from Synechocystis. J. Biol. Chem. 274, 189-195.
20. Lehman, A., Black, R. and Ecker, J.R. (1996) HOOKLESS1, an ethylene response gene, is required for differential cell elongation in the Arabidopsis hypocotyl. Cell, 85, 183-194.
21. Ljung, K., Hull, A.K., Kowalczyk, M., Marchant A., Celenza, J., Cohen, J.D. and Sandberg, G. (2002) Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana. Plant Mol. Biol. 50, 309-322.
22. Mikkelsen, M.D., Hansen, C.H., Wittstock, U. and Halkier, B.A. (2000) Cytochrome P450 CYP79B2 from Arabidopsis catalyzes the conversion of tryptophan to indole-3-acetaldoxime, a precursor of indole glucosinolates and indole-3-acetic acid. J. Biol. Chem. 275, 33712-33717.
23. Mikkelsen, M.D., Petersen, B.L., Olsen, C.E. and Halkier, B.A. (2002) Biosynthesis and metabolic engineering of glucosinolates. Amino Acids, 22, 279-295.
24. Mikkelsen, M.D., Petersen, B.L., Glawischnig, E., Jensen, A.B., Andreasson, E. and Halkier, B.A. (2003) Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways. Plant Physiol. 131, 298-308.
25. Naur, P., Petersen, B.L., Mikkelsen, M.D., Bak, S., Olsen, C.E. and Halkier, B.A. (2003) CYP83A1 and CYP83B1, two non-redundant enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis thaliana. Plant Physiol. 133, 63-72.
26. Nock, L.P. and Mazelis, M. (1986) The C-S lyases of higher plants: preparation and properties of homogeneous alliin lyase from garlic (Allium sativum). Arch. Biochem. Biophys. 249, 27-33.
27. Normanly, J. and Bartel, B. (1999) Redundancy as a way of life: IAA metabolism. Curr. Opin. Plant Biol. 2, 207-213.
28. Petersen, B.L., Chen, S., Hansen, C.H., Olsen, C.E. and Kalkier, B.A. (2002) Composition and content of glucosinolates in developing Arabidopsis thaliana. Planta, 214, 562-571.
29. Schwimmer, S. and Kjær, A. (1960) Purification and specificity of the C-S-lyase of Albizzia lophanta. Biochim. Biophys. Acta, 42, 316-324.
30. Seo, M., Akaba, S., Oritani, T., Delarue, M., Bellini, C., Caboche, M. and Koshiba, T. (1998) Higher activity of an aldehyde oxidase in the auxin-overproducing superroot1 mutant of Arabidopsis thaliana. Plant Physiol. 116, 687-693.
31. Wetter, L.R. and Chisholm, M.D. (1968) Sources of sulfur in various higher plants. Can. J. Biochem. 46, 931-935.
32. Winkler, R.G., Frank, M.R., Galbraith, D.W., Feyereisen, R. and Feldmann, K.A. (1998) Systematic reverse genetics of transfer-DNA-tagged lines of Arabidopsis. Plant Physiol. 118, 743-750.
33. Wittstock, U. and Halkier, B.A. (2002) Glucosinolate research in the Arabidopsis era. Trends Plant Sci. 7, 263-270.
34. Zhao, Y., Chrietensen, S.K., Fankhauser, C., Cashman, J.R., Cohen, J.D., Weigel, D. and Chory, J. (2001) A role for flavin monooxygenase-like enzymes in auxin biosynthesis. Science, 291, 306-309.
35. Zhao, Y., Hull, A.K., Gupta, N.R., Goss, K.A., Alonso, J., Ecker, J.R., Normanly, J., Chory, J. and Celenza, J.L. (2002) Trp-dependent auxin biosynthesis in Arabidopsis: involvement of cytochrome P450s CYP79B2 and CYP79B3. Genes Dev. 16, 3100-3112.