Single embryonic stem cell-derived embryoid bodies for gene screening

BioTechniques

Volumn 38, Issue 3, 2005, Pages 349-352

  Niimi, Masashi ^a   Kim, Mu Young ^a   Tao, Lian ^a   Liu, Hongmei ^b   Wu, Xiaoyun ^b   Kambayashi, Jun Ichi ^a   Yoshitake, Masuhiro ^a   Sun, Bing ^a  

^a OTSUKA PHARMACEUTICAL DEVELOPMENT AND COMMERCIALIZATION INC   (United States)

^b UNIVERSITY OF ALABAMA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; EMBRYONIC STEM CELL; GENE FUNCTION; GENE TARGETING; GENETIC SCREENING; MOUSE; NONHUMAN; PHENOTYPE; PLURIPOTENT STEM CELL;

ANIMALIA;

EID: 15244342282     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05383BM01     Document Type: Article

References (8)

1. Martin, G.R. 1981. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. USA 78:7634-7638.
2. Keller, G. 1995. In vitro differentiation of embryonic stem cells. Curr. Opin. Cell Biol. 7:862-869.
3. Wobus, A.M., H. Holzhausen, P. Jakel, and J. Schondeich. 1984. Characterization of a pluripotent stem cell line derived from a mouse embryo. Exp. Cell Res. 752:212-219.
4. Xiong, J.W., R. Battaglino, A. Leahy, and H. Stuhlmann. 1998. Large-scale screening for developmental genes in embryonic stem cells and embryoid bodies using retroviral entrapment vectors. Dev. Dyn. 212:181-197.
5. Hansen, J., T. Floss, P.V. Sloun, E.-M. Fuchtbauer, F. Vauti, H.-H. Arnold, F. Schnutgen, W. Wurst, et al. 2003. A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome. Proc. Natl. Acad. Sci. USA 100:9918-9922.
6. Keller, G., M. Kennedy, T. Papayannopoulou, and M. Wiles. 1993. Hematopoietic commitment during embryonic stem cell differentiation in culture. Mol. Cell Biol. 75:473-486.
7. Wartenberg, M., J. Gunther, J. Hescheler, and H. Sauer. 1998. The embryoid body as a novel in vitro assay system for antiangiogenic agents. Lab. Invest. 75:1301-1314.
8. Doevendans, P.A., S.W. Kubalak, R.-H. An, K.D. Becker, K.R. Chien, and R.S. Kass. 2000. Differentiation of cardiomyocytes in floating embryoid bodies is comparable to fetal cardiomyocytes. J. Mol. Cell Cardiol. 32:839-851.