A rapid procedure suitable to assess quantitatively the endocytosis of colloidal gold and its conjugates in cultured cells

Journal of Histochemistry and Cytochemistry

Volumn 46, Issue 10, 1998, Pages 1199-1201

  Andreu, José Enrique ^a   De Llano, José Javier Martín ^a   Moreno, Isabel ^a   Knecht, Erwin ^a  

^a CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

Author keywords

Colloidal gold; Cultured cells; Endocytosis; Inductively coupled plasma; Low density lipoproteins; Mass spectrometry; Quantitation

Indexed keywords

COLLOIDAL GOLD; LOW DENSITY LIPOPROTEIN;

ANIMAL CELL; ARTICLE; ATOMIC EMISSION SPECTROMETRY; CELL CULTURE; ELECTRON MICROSCOPY; ENDOCYTOSIS; FLUORESCENCE; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA;

EID: 15144357026     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549804601013     Document Type: Article

References (9)

1. Aniento F, Roche E, Cuervo AM, Knecht E (1993) Uptake and degradation of glyceraldehyde-3-phosphate dehydrogenase by rat liver lysosomes. J Biol Chem 268:10463-10470
2. Goldstein JL, Hobbs HH, Brown MS (1995) Familial hypercholesterolemia. In Scriver CR, Beaudet AL, Sly WS, Valle D, eds. The Metabolic and Molecular Bases of Inherited Disease. Vol 2. New York, McGraw-Hill,1981-2030
3. Hayat MA, ed. (1989,1991) Colloidal Gold: Principles, Methods and Applications. Vols 1-3. San Diego, Academic Press
4. Hayat MA (1992) Quantitation of immunogold labeling. Micron Microsc Acta 23:1-16
5. Jarvis FK, Gray AR (1992) Handbook of Inductively Coupled Plasma Mass Spectrometry. London, Royal Society of Chemistry
6. Kingsley DM, Krieger M (1984) Receptor mediated endocytosis of the low density lipoprotein: somatic cell mutants define multiple genes required for expression of surface-receptor activity. Proc Nad Acad Sci USA 81:5454-5458
7. Knecht E, Martínez-Ramón A, Grisolía S (1986) Electron microscopic localization of glutamate dehydrogenase in rat liver mitochondria by an immunogold procedure and monoclonal and polyclonal antibodies. J Histochem Cytochem 34:913-922
8. Martín de Llano JJ, Andreu EJ, Knecht E (1996) Use of inductively coupled plasma-mass spectrometry for the quantitation of the binding and uptake of colloidal gold-low density lipoprotein conjugates by cultured cells. Anal Biochem 243:210-217
9. Roth J (1996) The silver anniversary of gold: 25 years of the colloidal gold marker system for immunocytochemistry and histochemistry. Histochem Cell Biol 106:1-8