Extracellular pH modulates the activity of cultured human osteoblasts

Journal of Cellular Biochemistry

Volumn 68, Issue 1, 1998, Pages 83-89

  Kaysinger, Kathleen K ^a   Ramp, Warren K ^a  

^a CAROLINAS MEDICAL CENTER   (United States)

Author keywords

Alkaline phosphatase activity; Collagen synthesis; DNA synthesis; Glycolysis; Osteoblasts; pH

Indexed keywords

ALKALINE PHOSPHATASE; DNA; LACTIC ACID; PROLINE;

ARTICLE; CELL PROLIFERATION; COLLAGEN SYNTHESIS; CONTROLLED STUDY; CULTURE MEDIUM; DNA CONTENT; DNA SYNTHESIS; ENZYME ACTIVITY; GLYCOLYSIS; HUMAN; HUMAN CELL; MITOGENESIS; OSTEOBLAST; PH; PRIORITY JOURNAL; TRABECULAR BONE;

EID: 15144350863     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(19980101)68:1<83::AID-JCB8>3.0.CO;2-S     Document Type: Article

References (25)

1. Buskinsky DA (1995): Stimulated osteoclastic and suppressed osteoblastic activity in metabolic but not respiratory acidosis. Am J Physiol 268:C80-C88.
2. Bushinsky DA (1996): Metabolic alkalosis decreases bone calcium efflux by suppressing osteoclasts and stimulating osteoblasts. Am J Physiol 271:F216-F222.
3. Centrella M, McCarty TL, Canalis E (1988): Tumor necrosis factor-α inhibits collagen synthesis and alkaline phosphatase activity independently of its effect on deoxyribonucleic acid synthesis in osteoblast-enriched bone cell cultures. Endocrinology 123:1442-1448.
4. Chu S, Montrose MH (1995): Extracellular pH regulation in microdomains of colonic crypts: effects of short-chain fatty acids. Proc Natl Acad Sci USA 92:3303-3307.
5. Cohn DV, Forscher BK (1962): Aerobic metabolism of glucose by bone. J Biol Chem 237:615-618.
6. Galvin RJS, Ramp WK, Lenz LG (1991): Smokeless tobacco contains a nonnicotine inhibitor of bone metabolism. Toxicol Appl Pharmacol 115:1311-1314.
7. Kaila K, Panula P, Karhunen T, Heinonen E (1991): Fall in intracellular pH mediated by GABAA receptors in cultured rat astrocytes. Neurosci Lett 126:9-12.
8. Kaysinger KK, Ramp WK, Lang GJ, Gruber HE (1997): Comparison of human osteoblasts and osteogenic cells from heterotopic bone. Clin Orthop 342:181-191.
9. Kita K, Kawai K, Hirohata K (1987): Changes in bone marrow blood flow with aging. J Orthop Res 5:569-575.
10. Krieger NS, Sessler NE, Bushinsky DA (1992): Acidosis inhibits osteoblastic and stimulates osteoclastic activity in vitro. Am J Physiol 262:F442-F448.
11. Kubota M (1989): Study on proliferation and function of periodontal ligament fibroblasts and osteoblastic cells under hypoxia. Kokubyo Gakkai Zasshi 56:473-484.
12. + excretion. In: West JB (ed): "Best and Taylor's Physiological Basis for Medical Practice," 11th ed. Baltimore: Williams and Wilkins, pp 516-531.
13. Lowry OH, Roberts NR, Wu ML, Hixon WS (1957): The quantitative histochemistry of the brain: II. Enzyme measurements. J Biol Chem 207:19-37.
14. Lundholm L, Mohme-Lundholm E, Vamos N (1963): Lactic acid assay with L(+)lactic acid dehydrogenase from rabbit muscle. Acta Physiol Scand 58:243-249.
15. Neidlinger-Wilke C, Stalla I, Claes L, Brand R, Hoellen I, Rubenacker S, Arand M, Kinzl L (1995): Human osteoblasts from younger normal and osteoporotic donors in proliferation and TGF-beta release in response to cyclic strain. J Biomech 28:1411-1418.
16. Ori Y, Lee SG, Krieger NS, Bushinsky DA (1995): Osteoblastic intracellular pH and calcium in metabolic and respiratory acidosis. Kidney Int 47:1790-1796.
17. Ramp WK, Lenz LG, Kaysinger KK (1994): Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. Bone Miner 24:59-73.
18. Reeve J, Arlot M, Wootton R, Edouard C, Tellez M, Hesp R, Green JR, Meunier PJ (1988): Skeletal blood flow, iliac histomorphometry, and strontium kinetics in osteoporosis: A relationship between blood flow and corrected apposition rate. J Clin Endocrinol Metab 66:1124-1131.
19. Reolofsen J, Klein-Nulend J, Burger EH (1995): Mechanical stimulation by intermittent hydrostatic compression promotes bone-specific gene expression in vitro. J Biomech 28:1493-1503.
20. Robey PG, Termine JD (1985): Human bone cells in vitro. Calcif Tissue Int 37:453-460.
21. Sprague SM, Krieger NS, Bushinsky DA (1994): Greater inhibition of in vitro bone mineralization with metabolic than respiratory acidosis. Kidney Int 46:1199-1206.
22. Stanford CM, Stevens JW, Brand RA (1995): Cellular deformation reversibly depresses RT-PCR detectable levels of bone-related mRNA. J Biomech 28:1419-1427.
23. Tuncay OC, Ho D, Barker MK (1994): Oxygen tension regulates osteoblast function. Am J Orthod Dentofacial Orthop 105:457-463.
24. Whiteside LA, Simmons DJ, Lesker PA (1977): Comparison of regional bone blood flow in areas with differing osteoblastic activity in the rabbit tibia. Clin Orthop 124:267-270.
25. Yamaguchi DT, Huang JT, Ma D (1995): Regulation of gap junction intracellular communication by pH in MC3T3-E1 osteoblastic cells. J Bone Miner Res 10:1891-1899.