Identification of strongyle eggs from anthelmintic-treated horses using a PCR-ELISA based on intergenic DNA sequences

Parasitology Research

Volumn 95, Issue 4, 2005, Pages 287-292

  Hodgkinson, J E ^a   Freeman, K L ^b   Lichtenfels, J R ^c   Palfreman, S ^b   Love, S ^d   Matthews, J B ^e  

^a LIVERPOOL SCHOOL OF TROPICAL MEDICINE   (United Kingdom)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

^d UNIVERSITY OF GLASGOW   (United Kingdom)

^e MOREDUN RESEARCH INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHELMINTIC AGENT; BENZIMIDAZOLE; FENBENDAZOLE; PYRANTEL EMBONATE; SPACER DNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; CYATHOSTOMUM CATINATUM; CYLICOCYCLUS ASHWORTHI; CYLICOCYCLUS INSIGNE; CYLICOCYCLUS NASSATUS; CYLICOSTEPHANUS GOLDI; CYLICOSTEPHANUS LONGIBURSATUS; DEVELOPMENTAL STAGE; DNA SEQUENCE; DRUG EFFICACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES; HORSE; NONHUMAN; PARASITE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SAMPLING; STRONGYLOIDEA;

ANIMALS; ANTINEMATODAL AGENTS; DNA, HELMINTH; DNA, INTERGENIC; DRUG RESISTANCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FECES; FENBENDAZOLE; HORSES; PARASITE EGG COUNT; POLYMERASE CHAIN REACTION; PYRANTEL PAMOATE; STRONGYLE INFECTIONS, EQUINE; STRONGYLOIDEA;

ANIMALIA; CYATHOSTOMUM CATINATUM; CYLICOCYCLUS ASHWORTHI; CYLICOCYCLUS INSIGNE; CYLICOCYCLUS NASSATUS; CYLICOSTEPHANUS GOLDI; CYLICOSTEPHANUS LONGIBURSATUS; EQUIDAE; EQUUS CABALLUS; STRONGYLES;

EID: 15044356508     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-004-1289-z     Document Type: Article

References (32)

1. Barger IA (1986) The statistical distribution of trichostrongylid nematodes in grazing lambs. Int J Parasitol 15:645-649
2. Boersema JH, Borgsteede FHM, Eysker M, Saedt I (1995) The reappearance of strongyle eggs in faeces of horses treated with pyrantel embonate. Vet Q 17:18-20
3. Bucknell DG, Gasser RB, Beveridge I (1995) The prevalence and epidemiology of gastrointestinal parasites of horses in Victoria, Australia. Int J Parasitol 25:711-724
4. Chandler KJ, Collins MC, Love S (2001) Efficacy of a five-day course of fenbendazole in benzimidazole resistant cyathostomes. Vet Rec 147:661-662
5. Craven J, Bjorn H, Henriksen SA, Nansen P, Larsen M, Lendal S (1998) Survey of anthelmintic resistance on Danish horse farms using 5 different methods of calculating faecal egg count reduction. Equine Vet J 30:289-293
6. Crofton HD (1971) A model of host-parasite relationships. Parasitology 63:343-364
7. Dowdall SM, Proudman CJ, Klei TR, Mair T, Matthews (2004) Characterisation of IgG(T) serum antibody responses to two larval antigen complexes in horses naturally- or experimentally-infected with cyathostomins. Int J Parasitol 34:101-108
8. Duncan JL, Bairden KB, Abbot EM (1998) Elimination of mucosal cyathostome larvae by five daily treatments with fenbendazole. Vet Rec 142:268-271
9. Eysker M, Boersema JH, Kooman FNL (1992) The effect of ivermectin against inhibited early 3rd stage, late 3rd stage and 4th stage larvae and adult stages of the cyathostomes in Shetland ponies and expulsion of these helminths. Vet Parasitol 42:295-302
10. Gawor JJ (1995) The prevalence and abundance of internal parasites in working horses autopsied in Poland. Vet Parasitol 58:99-108
11. Giles CJ, Urquhart KA, Longstaffe JA (1985) Larval cyathostomiasis (immature trichoneme induced enteropathy), a report of 15 clinical cases. Equine Vet J 17:196-201
12. Hodgkinson JE, Love S, Lichtenfels JR, Palfreman S, Ramsey YH, Matthews JB (2001) Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses. Int J Parasitol 31:197-204
13. Hodgkinson JE, Lictenfels JR, Mair TS, Cripps P, Freeman KL, Ramsey YH, Love S, Matthews JB (2003) A PCR-ELISA for the identification of cyathostomin fourth-stage larvae from clinical cases of larval cyathostominosis. Int J Parasitol 33:1427-1435
14. Hung GC, Chilton NB, Beveridge I, McDonnell A, Lichtenfels JR, Gasser RB (1997) Molecular delineation of Cylicocyclus nassatus and C. ashworlhi (Nematoda: Strongylidae). Int J Parasitol 27:601-607
15. Kaplan RM (2002) Anthelmintic resistance in nematodes of horses. Vet Res 33:491-507
16. Kaye JN, Love S, Lichtenfels JR, Mckeand JB (1998) Comparative sequence analysis of the intergenic spacer (IGS) region of cyathostome species. Int J Parasitol 28:831-836
17. Klei TR, Chapman MR, French DD, Taylor HW (1993) Evaluation of ivermectin at an elevated dose against encysted equine cyathostome larvae. Vet Parasitol 47:99-106
18. Lichtenfels JR, Kharchenko VA, Sommer C, Ito M, (1997) Key characters for the microscopical identification of Cylicocyclus nassatus and Cylicocyclus ashworthi (Nematoda, Cyathostominae) of the horse, Equus caballus. J Helminthol Soc Wash 64:120-127
19. Love S, Mckeand JB (1997) Cyathostomosis: practical issues of treatment and control. Equine Vet Ed 9:253-256
20. Lyons E (2003) Population-S benzimidazole- and tetrahydropyrimidine- resistant small strongyles in a pony herd in Kentucky(1977-1999): effects of anthelmintic treatment on the parasites as determined in critical tests. Parasitol Res 91:407-411
21. Lyons ET, Tolliver SC (2003) Field test data on small strongyles in evaluation of activity of fenbendazole given once a day for 5 consecutive days to thoroughbred yearlings on two farms in Kentucky in 2002 and 2003. Parasitol Res 91:312-315
22. Mair TS, Pearson GR (1995) Multifocal non-strangulating intestinal infarction associated with larval cyathostomiasis in a pony. Equine Vet J 27:154-155
23. Murphy D, Love S (1997) The pathogenic effects of experimental cyathostome infections in ponies. Vet Parasitol 70:99-110
24. Ogbourne CP (1976) The prevalence, relative abundance and site distribution of nematodes of the subfamily Cyathostominae in horses killed in Britain. J Helminthol 50:203-214
25. Patterson P, Coles GC (2000) Winter transmission of equine nematodes. Vet Rec 147:251-252
26. Proudman C, Matthews JB (2000) Control of intestinal parasites in horses. Equine Prac 22:90-97
27. Reinemeyer CR, Smith SA, Gabel AA, Herd RP (1984) The prevalence and intensity of internal parasites of horses in the USA. Vet Parasitol 15:75-83
28. Silva AV, Costa HM, Santos HA, Carvalho RO (1999) Cyathostominae (Nematoda) parasites of Equus caballus in some Brazilian states. Vet Parasitol 86:15-21
29. Torbert BJ, Klei TR, Lichtenfels JR, Chapman MR (1986) A survey in Louisiana of intestinal helminths of ponies with little exposure to anthelmintics. J Parasitol 72:926-930
30. Uhlingher CA (1990) Effects of three anthelmintic schedules on the incidence of colic in horses. Equine Vet J 22:251-254
31. Urquhart GM, Armour J, Duncan JL, Dunn AM, Jennings FW (1996) Veterinary Parasitology, 2nd edn. Blackwell, Oxford, pp 276-277
32. Vanloon G, Deprez P, Muylle E, Sustronk B (1995) Larval cyathostomiasis as a cause of death in two regularly dewormed horses. J Vet Med 42:301-306