Quantification of melanoma micrometastases in sentinel lymph nodes using real-time RT-PCR

Journal of Investigative Dermatology

Volumn 124, Issue 3, 2005, Pages 633-637

  Giese, Thomas ^a,b   Engstner, Monika ^a   Mansmann, Ulrich ^a   Hartschuh, Wolfgang ^a   Arden, Bernhard ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b Institute of Immunology   (Germany)

Author keywords

Melan A; Quantitative PCR; Regional lymph node; Staging; Tyrosinase

Indexed keywords

MELAN A; MESSENGER RNA; MONOPHENOL MONOOXYGENASE;

ARTICLE; CANCER CELL; CANCER RECURRENCE; CANCER SURVIVAL; CONTROLLED STUDY; FOLLOW UP; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPH NODE METASTASIS; LYMPHADENECTOMY; MAJOR CLINICAL STUDY; MELANOMA; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENTINEL LYMPH NODE;

EID: 15044345520     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-202X.2005.23633.x     Document Type: Article

References (20)

1. Abrahamsen HN, Nexo E, Steiniche T, Hamilton-Dutoit SJ, Sorensen BS: Quantification of melanoma mRNA markers in sentinel nodes. Pre-clinical evaluation of a single-step real-time reverse transcriptase-polymerase chain reaction assay. J Mol Diagn 6:253-259, 2004
2. Balch CM, Soong SJ, Bartolucci AA, et al: Efficacy of an elective regional lymph node dissection of 1 to 4 mm thick melanomas for patients 60 years of age and younger. Ann Surg 224:255-263, 1996
3. Blaheta H-J, Ellwanger U, Schittek B, et al: Examination of regional lymph nodes by sentinel node biopsy and molecular analysis provides new staging facilities in primary cutaneous melanoma. J Invest Dermatol 114:637-642, 2000
4. Blaheta H-J, Schittek B, Breuninger H, et al: Detection of melanoma micrometastasis in sentinel nodes by reverse transcription-polymerase chain reaction correlates with tumor thickness and is predictive of micrometastatic disease in the lymph node basin. Am J Surg Pathol 23:822-828, 1999
5. Bostick PJ, Morton DL, Turner RR, et al: Prognostic significance of occult metastases detected by sentinel lymphadenectomy and reverse transcriptase-polymerase chain reaction in early-stage melanoma patients. J Clin Oncol 17:3238-3244, 1999
6. Carson KF, Wen D-R, Li P-X, Lana AM-A, Bailly C, Morton DL, Cochran AJ: Nodal nevi and cutaneous melanomas. Am J Surg Pathol 20:834-840, 1996
7. Chen Y-T, Stockert E, Tsang S, Coplan KA, Old LJ: Immunophenotyping of melanomas for tyrosinase: Implications for vaccine development. Proc Natl Acad Sci USA 92:8125-8129, 1995
8. Cochran AJ, Wen DR, Herschman HR: Occult melanoma in lymph nodes detected by antiserum to S-100 protein. Int J Cancer 34:159-163, 1984
9. Cochran AJ, Wen DR, Morton DL: Occult tumor cells in the lymph nodes of patients with pathological stage I malignant melanoma. An immunohistological study. Am J Surg Pathol 12:612-618, 1988
10. de Vries TJ, Smeets M, de Graaf R, et al: Expression of gp100, MART-1, tyrosinase, and S100 in paraffin-embedded primary melanomas and locoregional, lymph node, and visceral metastases: Implications for diagnosis and immunotherapy. A study conducted by the EORTC Melanoma Cooperative Group. J Pathol 193:13-20, 2001
11. Garbe C, Paul A, Kohler-Späth H, et al: Prospective evaluation of a follow-up schedule in cutaneous melanoma patients: Recommendations for an effective follow-up strategy. J Clin Oncol 21:520-529, 2003
12. Hatta N, Takata M, Takehara K, Ohara K: Polymerase chain reaction and immunohistochemistry frequently detect occult melanoma cells in regional lymph nodes of melanoma patients. J Clin Pathol 51:597-601, 1998
13. Hofmann U, Szedlak M, Rittgen W, Jung EG, Schadendorf D: Primary staging and follow-up in melanoma patients-monocenter evaluation of methods, costs and patient survival. Br J Cancer 87:151-157, 2002
14. Kahle B, Hoffend J, Hartschuh W, Petzoldt D: Sentinel lymph node sonography in malignant melanoma. Hautarzt 51:915-919, 2000
15. Kirkwood JM: Adjuvant IFNα2 therapy of melanoma. Lancet 351:1901-1903, 1998
16. Lukowsky A, Bellmann B, Ringk A, Winter H, Audring H, Fenske S, Sterry W: Detection of melanoma micrometastases in the sentinel lymph node and in nonsentinel nodes by tyrosinase polymerase chain reaction. J Invest Dermatol 113:554-559, 1999
17. Morton DL, Davtyan DG, Wanek LA, Foshag LJ, Cochran AJ: Multivariate analysis of the relationship between survival and the microstage of primary melanoma by Clark level and Breslow thickness. Cancer 71:3737-3743, 1993
18. Morton DL, Wen DR, Wong JH, et al: Technical details of intraoperative lymphatic mapping for early stage melanoma. Arch Surg 127:392-399, 1992
19. Shivers SC, Wang X, Li W, et al: Molecular staging of malignant melanoma: Correlation with clinical outcome. JAMA 280:1410-1415, 1998
20. Wang X, Heller R, VanVoorhis N, et al: Detection of submicroscopic lymph node metastases with polymerase chain reaction in patients with malignant melanoma. Ann Surg 220:768-774, 1994