Detection of Mycoplasma mycoides subspecies mycoides SC in bovine lung and lymph node tissues by culture, sandwich ELISA and polymerase chain reaction systems

Research in Veterinary Science

Volumn 78, Issue 3, 2005, Pages 199-205

  Bashiruddin, John B ^a,f   de Santis, Paola ^b   Persson, Anja ^c   Ball, Hywel ^d   Regalla, José ^e  

^a Abruzzo e del Molise 'G Caporale' ^*  (United Kingdom)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL ABRUZZO E DEL MOLISE G CAPORALE   (Italy)

^c Statens Veterinarmedinska Anstalt   (Sweden)

^d AGRI FOOD AND BIOSCIENCES INSTITUTE   (United Kingdom)

^e Laboratorio Nacional de Investigacao Veterinaria   (Portugal)

^f ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Bovine; CBPP; Diagnosis; MmmSC; Mycoplasma mycoides; PCR; PCR LIF; PleuroTRAP; sELISA

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL DNA; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY SPECIFICITY; ANTIGEN DETECTION; ARTICLE; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM DETECTION; CATTLE DISEASE; CLINICAL EXAMINATION; CONTAGIOUS PLEUROPNEUMONIA; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; HISTOPATHOLOGY; INTERMETHOD COMPARISON; LUNG PARENCHYMA; MEDIASTINUM LYMPH NODE; MYCOPLASMA MYCOIDES; NONHUMAN; PATHOGENESIS; POLYMERASE CHAIN REACTION; PORTUGAL; SENSITIVITY ANALYSIS;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CATTLE; CATTLE DISEASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; LUNG; LYMPH NODES; MYCOPLASMA MYCOIDES; PLEUROPNEUMONIA, CONTAGIOUS; POLYMERASE CHAIN REACTION; PORTUGAL;

ANIMALIA; BOS TAURUS; BOVINAE; MYCOPLASMA; MYCOPLASMA MYCOIDES;

EID: 14844327745     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rvsc.2004.09.004     Document Type: Article

References (28)

1. A. Akane K. Matsubara H. Nakamura S. Takahashi K. Kimura Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification J. Forensic Sci. 39 1994 362-372
2. Ayling, R., Regalla, J., Spencer, Y., Nicholas, R., De Santis, P., 1998. Investigation on a CBPP outbreak in Portugal. COST 826. Agriculture and Biotechnology. Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics, pp. 117-120
3. H.J. Ball D. Finlay G.A.C. Reilly Sandwich ELISA detection of Mycoplasma bovis in pneumonic calf lungs and nasal swabs Vet. Record 135 1994 531-532
4. Bashiruddin, J.B., 1996. Observations from outbreaks of CBPP in Europe and Africa. In: Frey, J., Sarris, K. (Eds.), COST 826 Agriculture and Biotechnology Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics. EUR 16934, Luxembourg, pp. 150-154
5. J.B. Bashiruddin P. De Santis A. Vacciana F.G. Santini Detection of Mycoplasma mycoides subspecies mycoidesSC in clinical material by a rapid colorimetric PCR Mol. Cell. Probe. 13 1999 23-28
6. J.B. Bashiruddin R.A.J. Nicholas F.G. Santini R.A. Ready M.J. Woodward T.K. Taylor Use of the polymerase chain reaction to detect mycoplasma DNA in cattle with contagious bovine pleuropneumonia Vet. Rec. 134 1994 240-241
7. J.B. Bashiruddin F.G. Santini P. De Santis M.C. Visaggio G. Di Francesco A. D'angelo R.A.J. Nicholas Detection of Mycoplasma mycoides subspecies mycoides in tissues from an outbreak of contagious bovine pleuropneumonia by culture, immunohistochemistry and polymerase chain reaction Vet. Rec. 145 1999a 271-274
8. J.B. Bashiruddin T.K. Taylor A.R. Gould A PCR-based test for the specific identification of Mycoplasma mycoides subspecies mycoidesSC J. Vet. Diagn. Invest. 6 1994b 428-434
9. CABI, 2004. Contagious Bovine Pleuropneumonia. In: Animal Health and Production Compendium. Wallingford, UK. CAB International. Available from:
10. P.F. Guadagnini F. De Simone G.F. Panini A. Gaffuri M. Bugnatti M. Finazzi G. Mandelli G. Sironi A. Belloli La pleuropolmonite contagiosa dei bovini (PPCB). Selezione Veterinaria 32 1991 3-31
11. Johansson, K.E., Bölske, G., Pettersson, B., 1996. The phylogeny of the Mycoplasma mycoides cluster based on 16S rRNA sequence analysis. In: Frey, J., Sarris, K. (Eds.), COST 826. Agriculture and Biotechnology. Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics. EUR 16934, Luxembourg, pp. 16-18
12. M.A.G. Machado M.da.C. Contreiras V. Atalaia A. Penha Goncalves Analise genomica de micoplasmas do "grupo M. mycoides" por endonucleases de restricao Revista Portuguesa de Ciencias Veterinarias LXXXVII 1992 66-82
13. R.A.J. Nicholas J.B. Bashiruddin Mycoplasma mycoides subspecies mycoides (small colony variant): The agent of contagious bovine pleuropneumonia and member of the "Mycoplasma mycoides cluster" J. Comp. Pathol. 113 1995 1-27
14. R.A.J. Nicholas N. Palmer Contagious bovine pleuropneumonia in Europe State Vet. J. 4 1994 14-16
15. R.A.J. Nicholas F.G. Santini K.M. Clark M.A. Palmer P. De Santis J.B. Bashiruddin A comparison of serological tests and gross lung pathology for detecting contagiuos bovine pleuropneumonia in two groups of Italian cattle Vet. Rec. 139 1996 89-93
16. R. Nicholas J. Bashiruddin R. Ayling R. Miles Contagious bovine pleuropneumonia: A review of recent developments Vet. Bull. 70 2000 827-838
17. OIE, 1997. Revue Scientifique et Technique. Office International des Epizooties 16, 898-904
18. OIE, 2004. OIE HANDISTATUS II, updated July 2004, Available from:
19. Perreau, P., 1983. Isolation procedures for the diagnosis of contagious bovine pleuropneumonia. In: Hall, S.A. (Ed.), The Diagnosis of Contagious Bovine Pleuropneumonia and Other Infections with Mycoplasma mycoides subsp. mycoides. C.E.C. Publications EUR 8654, Luxembourg, pp. 18-26
20. A. Persson B. Pettersson G. Bolske K.-E. Johansson Diagnosis of contagious bovine pleuropneumonia by PCR-laser-induced fluorescence and PCR-restriction endonuclease analysis based on the 16S rRNA genes of Mycoplasma mycoides subsp. mycoidesSC J. Clin. Microbiol. 37 1999 3815-3821
21. B. Petterson T. Leitner M. Ronaghi G. Bölske M. Uhlen K.E. Johansson Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons J. Bacteriol. 178 1996 4131-4142
22. A. Provost P. Perreau B. Bréard C. Le Goff J.L. Martel G.S. Cottew Contagious bovine pleuropneumonia Rev. Sci. Tech. OIE 6 1987 625-679
23. J. Regalla Epidemiological aspects of contagious bovine pleuropneumonia in Portugal Repositorio de Trabalhos do Laboratorio Nacional Investigacao Veternaria (Lisbon) 16 1984 15-18
24. F. Rodriguez H.J. Ball D. Findlay D. Campbell D.P. Mackie Detection of Mycoplasma mycoides subspecies mycoides by monoclonal antibody-based sandwich ELISA Vet. Microbiol. 51 1996 69-76
25. Rweyemamu, M.M., Benkirane, S., 1996. Global impact of infections with organisms of the "Mycoplasma mycoides cluster" in ruminants In: Frey, J., Sarris, K. (Eds.), COST 826 Agriculture and Biotechnology. Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics, EUR 16934, Luxembourg, pp. 1-11
26. E. Scanziani G. Sironi M. Finazzi V. Grieco S. Paltrinieri G. Mandelli Alterazioni linfonodali in corso di pleuropolmonite contagiosi del bovino (PPCB) Atti della Societa Italiani di Buiatria 23 1991 417-429
27. T.K. Taylor J.B. Bashiruddin A.R. Gould Relationship between members of the Mycoplasma mycoides cluster as shown by DNA probe and sequence analysis Int. J. Syst. Bacteriol. 42 1992 594-597
28. E.A. Ter Laak Contagious bovine pleuropneumonia. A review Vet. Quart. 14 1992 104-110