Detection of Erwinia amylovora in and on apple tissue using PCR

Acta Horticulturae

Volumn 590, Issue , 2002, Pages 163-166

  Pulawska, J ^a   Sobiczewski, P ^a  

^a RESEARCH INSTITUTE OF POMOLOGY AND FLORICULTURE   (Poland)

Author keywords

Detection; Erwinia amylovora; Fire blight; PCR inhibitors; Pesticides

Indexed keywords

EID: 14844309926     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2002.590.23     Document Type: Conference Paper

References (5)

1. Llop, P., Bonaterra, A., Penalver, J. and Lopez, M. 2000. Development of a highly sensitive nested-PCR procedure using a single closed tube for detection of Erwinia amylovora in asymptomatic plant material. Appl. Environ. Microbiol. 66, 2071-2078.
2. Maes, M., Garbeva, P. and Crepel, C. 1996. Identification and sensitive endophytic detection of fire blight pathogen Erwinia amylovora with 23S ribosomal DNA sequences and the polymerase chain reaction. Plant Pathology 45, 1136-1149.
3. Pulawska, J. 1997. Detection of Agrobacterium tumefaciens in soil using PCR. In: Diagnosis and Identification of Plant Pathogens. (eds. H. .W. Dehne, G. Adam, M. Diekmann, J. Frahm, A. Mauler . Machnik, P. van Halteren) Kluwer Academic Publishers. Pp 457-460.
4. Pulawska, J., Maes, M., Deckers, T. and Sobiczewski, P. 1997. The influence of pesticide contamination on detection of epiphytic Erwinia amylovora using PCR. Med. Fac. Landbouww. Univ. Gent. 62/3b, 959-962.
5. Pulawska, J. and Sobiczewski, P. 2001. Semi-nested PCR-based method for detection of Agrobacterium tumefaciens in soil. In: Plant Pathogenic Bacteria (ed. S.H. De Boer) Kluwer Acad. Publ. Pp 438-440.