Kinetic studies with alkaline phosphatase in the presence and absence of inhibitors and divalent cations

Biochemistry and Molecular Biology Education

Volumn 30, Issue 6, 2002, Pages 401-407

  Dean, Rob L ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

Alkaline phosphatase; Divalent cation cofactors; Enzyme inhibition; Enzyme kinetics

Indexed keywords

ALKALINE PHOSPHATASE; DIVALENT CATION; MAGNESIUM; PHENYLALANINE; ZINC;

ARTICLE; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; HUMAN; HYDROLYSIS; MEDICAL EDUCATION; MEDICAL STUDENT; PH;

EID: 14844299261     PISSN: 14708175     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmb.2002.494030060138     Document Type: Article

References (20)

1. P. Abramoff, R. G. Thomson (1996) An experimental Approach to Biology, 2nd ed., W. H. Freeman, San Francisco.
2. G. Litvak (1960) Experimental Biochemistry. A Laboratory Manual, Wiley, New York.
3. R. P. Kuniewicz, in K. P. Heston, B. Y. Stewart, Eds. (1991) PEW Undergraduate Laboratories in Biology. A Collection of Introductory Experiments in Cellular and Molecular Biology, Biology Curriculum Development Group, PEW Science Program in Undergraduate Education, Mid-Atlantic Cluster, pp. 1-11.
4. J. M. Clark, R. L. Switzer (1977) Experimental Biochemistry, 2nd ed., W. H. Freeman, San Francisco.
5. F. Ranaldi, P. Vanni, E. Giachetti (1999) What students must know about the determination of enzyme kinetic parameters, Biochem. Educ. 27, 87-91.
6. C. G. Whiteley (2000) Enzyme kinetics: partial and complete uncompetitive inhibition, Biochem. Educ. 28, 144-147.
7. N. C. Price, L. Stevens (1989) Fundamentals of Enzymology, 2nd ed., Oxford University Press, Oxford.
8. A. Cornish-Bowden, C. W. Wharton (1988) Enzyme Kinetics, IRL Press, Oxford.
9. N. K. Ghosh, W. H. Fishman (1966) On the mechanism of inhibition of intestinal alkaline phosphatase by L-phenylalanine, J. Biol. Chem. 241, 2516-2522.
10. J.-P. Bretaudiere, T. Spillman, in H. V. Bergmeyer, Ed. (1984) Methods of Enzymatic Analysis, Vol. 4, Verlag Chemie, Weinheim, Germany, pp. 75-92.
11. M. Fosset, D. Chappelet-Tordo, M. Lazdunski (1974) Intestinal alkaline phosphatase. Physical properties and quaternary structure, Biochemistry 13, 1783-1788.
12. R. L. Dean (2002) It's laboratory class time, do you known what your buffer is doing?, Am. Biol. Teach. 64, 540-547.
13. R. F. Boyer (2000) Modern Experimental Biochemistry, 3rd ed., Benjamin/Cummings, San Francisco.
14. A. J. Ninfa, B. P. Ballou (1998) Fundamental Laboratory Approaches for Biochemistry and Biotechnology, Fitzgerald, Bethesda, MD.
15. R. L. L. Switzer, L. F. Garrity (1999) Experimental Biochemistry, 3rd ed., Freeman, New York.
16. J. M. Berg, J. L. Tymoczko, L. Stryer (2002) Biochemistry, 5th ed., Freeman, New York.
17. R. F. Boyer (2002) Concepts in Biochemistry with the interactive Concepts in Biochemistry CD-ROM, 2nd ed., John Wiley, New York.
18. D. Voet, J. G. Voet, Pratt, C. W. (2001) Fundamentals of Biochemistry, Upgrade Edition, Wiley, New York.
19. C. K. Mathews, K. E. Van Holde, K. G. Ahern (2000) Biochemistry, 3rd ed., Benjamin Cummings, Redwood City, CA.
20. A. L. Lehninger, D. L. Nelson, M. M. Cox (2000) Principles of Biochemistry, 3rd ed., Freeman, San Francisco.