Two-photon excitation fluorescence pH detection using 2,3- dicyanohydroquinone: A spectral ratiometric approach

Journal of Microscopy

Volumn 217, Issue 3, 2005, Pages 260-264

  Jöbsis, Paul D ^a   Combs, C A ^b   Balaban, R S ^a  

^a National Institutes of Health   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Cellular pH; Dicyanohydroquinone; Dihydroxyphthalonitrile; Spectral calibration; Spectral imaging; Two photon

Indexed keywords

CALIBRATION; CELL CULTURE; EMISSION SPECTROSCOPY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; PH; PHOTONS;

CELLULAR PH; CELLULARS; DICYANOHYDROQUINONE; DIHYDROXYPHTHALONITRILE; IN-VITRO; RATIOMETRIC; SPECTRAL CALIBRATION; SPECTRAL IMAGING; TWO PHOTON; TWO-PHOTON EXCITATION FLUORESCENCE MICROSCOPY;

PROBES;

3,6 DIHYDROXYPHTHALONITRILE; HYDROQUINONE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CALIBRATION; CELL PH; FIBROBLAST; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 14744284099     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2005.01442.x     Document Type: Article

References (7)

1. 2+ in optically dense and biologically relevant samples. Appl. Spectrosc. 54, 455-463.
2. Denk, W., Strickler, J. & Webb, W. (1990) Two-photon excitation in laser scanning microscopy. Science, 248, 73-76.
3. Kierdaszuk, B., Malak, H., Gryczynski, I., Callis, P. & Lakowicz, J. (1996) Fluorescence of reduced nicotinamides using one- and two-photon excitation. Biophys. Chem. 62, 1-13.
4. Kurtz, I. & Balaban, R. (1986) Fluorescence emission spectroscopy of 1,4-dihydroxyphthalonitrile. Biophys. J. 48, 499-508.
5. Owen, C. (1992) Comparison of spectrum-shifting intracellular pH probes 5′ (and 6′)-carboxy-10-dimethylamino-3-hydroxyspiro[7H-benzo[c] xanthene-7,1′(3′H)-isobenzofuran]-3′-one and 2′, 7′-biscarboxyethyl-5 (and 6)-carboxyfluorescein. Anal. Biochem. 204, 65-71.
6. Szmacinski, H., Gryczynski, I. & Lakowicz, J. (1996) Three-photon induced fluorescence of the calcium probe Indo-1. Biophys. J. 70, 547-555.
7. Wieder, E., Hang, H. & Fox, M. (1993) Measurement of intracellular pH using flow cytometry with carboxy-SNARF-1. Cytometry, 8, 916-921.