A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures

Extremophiles

Volumn 9, Issue 1, 2005, Pages 75-79

  Gonzalez, Juan M ^a   Saiz Jimenez, Cesareo ^a  

^a INSTITUTO DE RECURSOS NATURALES Y AGROBIOLOGÍA DE SEVILLA   (Spain)

Author keywords

DNA homology; DNA hybridization; DNA relatedness; Melting curve; Pyrococcus; SYBR Green I; Thermal denaturation temperature; Thermococcus

Indexed keywords

DNA; ORGANIC COMPOUND; PROTEIN; SYBR GREEN I;

ARTICLE; CHEMISTRY; FLUOROMETRY; GENETICS; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION; PYROCOCCUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE; THERMOCOCCUS; THERMODYNAMICS; TIME;

DNA; FLUOROMETRY; NUCLEIC ACID HYBRIDIZATION; ORGANIC CHEMICALS; PROTEINS; PYROCOCCUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TEMPERATURE; THERMOCOCCUS; THERMODYNAMICS; TIME FACTORS;

PYROCOCCUS; THERMOCOCCUS;

EID: 14644440594     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00792-004-0417-0     Document Type: Article

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