Characterization of the basic major royal jelly protein MRJP2 of honeybee (Apis mellifera) and its preparation by heterologous expression in E. coli

Biologia

Volumn 54, Issue 6, 1999, Pages 733-739

  Bíliková, Katarína ^a   Klaudiny, Jaroslav ^a   Šimúth, Jozef ^a  

^a INSTITUTE OF CHEMISTRY   (Slovakia)

Author keywords

Heterologous expression; Honeybee; Ion exchange chromatography; Isoelectrofocusing; Protein purification; Recombinant protein; Royal jelly proteins

Indexed keywords

APIS MELLIFERA; ESCHERICHIA COLI;

EID: 14644430687     PISSN: 00063088     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. BAUER, L., KOHLICH, A., HIRSCHWEHR, R., SIEMANN, U., EBNER, H., SCHEINER, O., KRAFT, D. & EBNER, C. 1996. Food allergy to honey: Pollen or bee products? J. Allergy Clin. Immunol. 97: 65 73.
2. FARABAUGH, P. J. 1978. Sequence of the iacl gene. Nature 274: 765-769.
3. HANES, J. & SIMüTH, J. 1992. Identification and partial characterization of the major royal jelly protein of the honey bee (Apis mellifera L.). J. Apic. Res. 31: 22-26.
4. HIRANO, H. & WATANABE, T. 1990. Microsequencing of proteins electrotransferred on to immobilizing matrices from polyacrylamide gel electrophoresis: application to an insoluble protein. Electrophoresis 11: 573-580.
5. JUDOVÁ, J., KLAUDINY, J. & SIMÚTH, J. 1998. Preparation of recombinant most abundant protein MRJP1 of royal jelly. Biologia, Bratislava 53: 777-784.
6. KIMURA, Y., KAJIYAMA, S., KANAEDA, .J., IZUKAWA, T. & YONEKURA, M. 1996. N-linked sugar chain of 55-kDa royal jelly glycoprotein. Biosci. Biotech. Biochem. 60: 2099-2102.
7. KLAUDINY, J., HANES, J., KULIFAJOVà, J., ALBERT, S. fc SIMüTH, J. 1994. Molecular cloning of two cDNAs from the head of the nurse honey bee (Api's mellifera L.) coding for related proteins of royal jelly. J. Apic. Res. 33: 105-111.
8. LAEMMLI, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Mature 227: 680-685.
9. LEE, S. & RASHEED, S. 1990. A simple procedure for maximum yield of high quality plasmid DNA. Biotechniques 9: 676-679.
10. QIAGEN Manual Instruction, 1995. The QIAexpressionist - the high level expression & protein purification system. QIAGEN GmbH, QIAGEN Inc. and QIAGEN Ltd.
11. QIAGEN Handbook, 1992. DNA extraction from agarose gels. QIAGEN GmbH, QIAGEN Inc, 11-17.
12. SAMBROOK, J., FRITSCH, E. F. & MANIATIS, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York.
13. SOHMITZOVÂ, J., KLAUDINY, J., ALBERT, S.. SCHRö DER, W., SCHRECKENGOST, W., HANES, J., JUDOVa, .J. &. SIMÜTH, J. 1998. A new family of major royal jelly proteins of honeybee Apis mellifera L. Cell. Mol. Life Sei. 54: 1020-1030.
14. STIEGE. W. & ERDMANN, V. A. 1995. The potentials of the in vitro protein biosynthesis system. .J. Biotechnol. 41: 81-90.
15. STOTT. D. .1. 1989. Immunoblotting and dot blot blotting. J. Immunol. Meth. 119: 153-187.
16. TAKENAKA, T. 1982. Chemical composition of royal jelly. Honeybee Sei. 3: 69-74.
17. THIEN. F. C. K., LEUNG, R., BALDO, B. A., WEINER, J. A., PLOMLEY, R. fc CZARNY, D. 1996. Asthma and anaphylaxis induced by royal jelly. Clin. Exp. Allergy 26: 216-222.