Inositol tripyrophosphate: A new membrane permeant allosteric effector of haemoglobin

Bioorganic and Medicinal Chemistry Letters

Volumn 15, Issue 6, 2005, Pages 1605-1608

  Fylaktakidou, Konstantina C ^a,e   Lehn, Jean Marie ^a   Greferath, Ruth ^b,c   Nicolau, Claude ^c,d  

^a UNIVERSITÉ LOUIS PASTEUR   (France)

^b IRCAD   (France)

^c AC IMMUNE SA   (Switzerland)

^d TUFTS UNIVERSITY   (United States)

^e DEMOCRITUS UNIVERSITY OF THRACE   (Greece)

Author keywords

Haemoglobin; Inositol tripyrophosphate; myo Inositol hexakis phosphate; Red blood cells

Indexed keywords

HEMOGLOBIN; INOSITOL DERIVATIVE; INOSITOL TRIPYROPHOSPHATE; OXYGEN; OXYHEMOGLOBIN; PYROPHOSPHORIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ALLOSTERISM; ARTICLE; BLOOD SAMPLING; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; DRUG BINDING; DRUG SYNTHESIS; ERYTHROCYTE MEMBRANE; HUMAN; IN VITRO STUDY; MEMBRANE TRANSPORT; NONHUMAN; OXYGEN AFFINITY; OXYGEN DISSOCIATION CURVE; OXYGEN TENSION;

EID: 14644430288     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.01.064     Document Type: Article

References (15)

1. R.F. Irvine, and M.J. Schell Nature Rev. Mol. Cell Biol. 2 2001 327
2. E. Skoglund, N.-G. Carlsson, and A.-S. Sandberg J. Agric. Food Chem. 46 1998 1877
3. J.P. Heslop, R.F. Irvine, A.H. Tashjian Jr., and M. Berridge J. Exp. Biol. 119 1985 395
4. R. Benesch, R.E. Benesch, and C.Y. Yu Proc. Natl. Acad. Sci. U.S.A. 59 1968 526
5. R.E. Benesch, R. Edalji, and R. Benesch Biochemistry 16 1977 2594
6. B. Teisseire, C. Ropars, M.O. Vallez, R.A. Herigault, and C. Nicolau J. Appl. Physiol. 58 1985 1810
7. B. Teisseire, C. Ropars, M.C. Villeréal, and C. Nicolau Proc. Natl. Acad. Sci. U.S.A. 84 1987 6894
8. U. Brüggeman, E.C. Roux, J. Hannig, and C. Nicolau Transfusion 35 1995 478
9. S.P. Vincent, J.-M. Lehn, J. Lazarte, and C. Nicolau Bioorg. Med. Chem. 10 2002 2825
10. L.F. Johnson, and M.E. Tate Can. J. Chem. 47 1969 63
11. + column. The eluate was concentrated in vacuum to give hexasodium salt of myo-inositol 1,6:2,3:4,5-tripyrophosphate 5. All data were found identical to the reported ones
12. D.H. Rammler, and H.G. Khorana J. Am. Chem. Soc. 84 1962 3112
13. D. Söll, and H.G. Khorana J. Am. Chem. Soc. 87 1965 360
14. 50 shifts immediately. Compound solution was adjusted at pH = 6.9-7.1. For the incubation of whole blood with the effectors, the osmolarity of the compound solutions was adjusted at 290-310 mOsM and pH at 7. Effector solutions (up to 240 mM) and whole blood at 1:1 vol/vol ratios were incubated at 37°C for 60 min. Following incubation the blood cells were washed three times with 20 mM bis-Tris buffer, pH = 7, 308 mOsM. No lysis of the incubated RBC was observed. The oxy-Hb dissociation curves were recorded using the Hemox Analyzer (PD Marketing, West Sussex PO 207 RH, NK) and a HAS software from TCS Scientific (New Hope, PA). Reading solution consisted of an aliquot of RBCs to set instrument parameters and 20 mM Hemox buffer (Osm = 310 ± 10 mOsM, pH 7.1-7.2) at 37°C (concentration: 40-60 μL RBCs in 3 mL buffer)
15. K. Gersonde, and C. Nicolau Naturwissenschaften 66 1979 567