Analysis of the function of a hyperthermophilic endoglucanase from Pyrococcus horikoshii that hydrolyzes crystalline cellulose

Extremophiles

Volumn 9, Issue 1, 2005, Pages 37-43

  Kashima, Yasuhiro ^a,c   Mori, Kazushige ^a   Fukada, Harumi ^b   Ishikawa, Kazuhiko ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b Osaka Prefecture University   (Japan)

^c Thermostable Enzyme Laboratory Ltd   (Japan)

Author keywords

Active site; Cellulase; Chitinase; Disulfide bond; Endoglucanase; Hyperthermophile; Site directed mutagenesis

Indexed keywords

CELLULASE; CELLULOSE; CHITINASE; CYSTEINE; DISULFIDE; PRIMER DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BAGG ALBINO MOUSE; BINDING SITE; CATALYSIS; CHEMISTRY; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME ACTIVE SITE; ENZYMOLOGY; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; METABOLISM; MOLECULAR GENETICS; MOUSE; MUTATION; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE; PYROCOCCUS HORIKOSHII; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TEMPERATURE; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BINDING SITES; BLOTTING, WESTERN; CALORIMETRY, DIFFERENTIAL SCANNING; CATALYSIS; CATALYTIC DOMAIN; CELLULASE; CELLULOSE; CHITINASE; CYSTEINE; DISULFIDES; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; OPEN READING FRAMES; PLASMIDS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; PYROCOCCUS HORIKOSHII; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

ACIDOTHERMUS; ARCHAEA; ESCHERICHIA COLI; PYROCOCCUS FURIOSUS; PYROCOCCUS HORIKOSHII;

EID: 14644419633     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00792-004-0418-z     Document Type: Article

References (11)

1. Ando S, Ishida H, Kosugi Y, Ishikawa K (2002) Hyperthermostable endoglucanase from Pyrococcus horikoshii. Appl Environ Microbiol 68:430-433
2. Bauer MW, Driskill LE, Callen W, Snead MA, Mathur EJ, Kelly RM (1999) An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes β-1,4 bonds in mixed-linkage (1 → 3), (1 → 4)-β-D-glucans and cellulose. J Bacteriol 259:284-290
3. Chhabra SR, Kelly RM (2002) Biochemical characterization of Thermotoga maritima endoglucanase Cel74 with and without a carbohydrate binding module (CBM). FEBS Lett 531:375-380
4. Hiromi K, Takahashi Y, Ono S (1963) Kinetics of hydrolytic reaction catalyzed by crystalline bacterial α-amylase. The influence of temperature. Bull Chem Soc Jpn 36:563-569
5. Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E, Nakamura H, Katayanagi K, Morikawa K, Ikehara M (1991) Stabilization of Escherichia coli ribonuclease H by introduction of an artificial disulfide bond. J Biol Chem 266:6038-6044
6. Lee SG, Pancholi V, Fischetti VA (2002) Characterization of a unique glycosylated anchor endopeptidase that cleave the LPXTG sequence motif of cell surface proteins of Gram-positive bacteria. J Biol Chem 277:46912-46922
7. Matsumura M, Signor G, Matthews BW (1989) Substantial zincrease of protein stability by multiple disulphide bonds. Nature 342:291-293
8. Michael EH, William AS, Melvin PT, Karel G (1994) Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068. US Patent 5275944
9. Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H (1986) Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction. Cold Spring Harb Symp Quant Biol 51:263-273
10. Sakon J, Adney WS, Himmel ME, Thomas SR, Karplus PA (1996) Crystal structure of thermostable family 5 endocellulase El from Acidothermus cellulolyticus in complex with cellotetraose. Biochem 35:10648-10660
11. Wakarchuk WW, Sung WL, Campbell RL, Cunningham A, Watson DC, Yaguchi M (1994) Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds. Protein Eng 7:1379-1386