Preparative HPLC for purification of four isomeric bioactive saponins from the seeds of Aesculus chinensis

Journal of Liquid Chromatography and Related Technologies

Volumn 28, Issue 5, 2005, Pages 763-773

  Wei, Feng ^a   Ma, Lin Yun ^a   Cheng, Xian Long ^a   Lin, Rui Chao ^a   Jin, Wen Tao ^b   Khan, Ikhlas A ^b   Lu, Jian Qiu ^c  

^a NATIONAL INSTITUTE FOR THE CONTROL OF PHARMACEUTICAL AND BIOLOGICAL PRODUCTS   (China)

^b UNIVERSITY OF MISSISSIPPI   (United States)

^c BEIJING UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Aesculus chinensis; escin Ia; escin Ib; isoescin Ia; isoescin Ib; Preparatory HPLC; Stability studies

Indexed keywords

ACETIC ACID; ISOMERS; METHANOL; PLANTS (BOTANY); PURIFICATION; SAPONIFICATION; SEED; SOLVENTS; WATER;

AESCULUS CHINENSIS; BIOACTIVE SAPONIN COMPOUNDS; CRUDE EXTRACT; MOBILE PHASES;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ACETIC ACID; ESCIN; ESCIN IA; ESCIN IB; ISOESCIN IA; ISOESCIN IB; METHANOL; SAPONIN; UNCLASSIFIED DRUG; WATER;

AESCULUS HIPPOCASTANUM; ARTICLE; CHEMICAL ANALYSIS; CHEMICAL STRUCTURE; DRUG ACTIVITY; DRUG PURIFICATION; DRUG STABILITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIPPOCASTANACEAE; ISOLATION AND PURIFICATION; LARGE SCALE PRODUCTION; PHYTOCHEMISTRY; PLANT SEED;

AESCULUS; AESCULUS CHINENSIS; SAPINDACEAE;

EID: 14644413463     PISSN: 10826076     EISSN: None     Source Type: Journal    
DOI: 10.1081/JLC-200048904     Document Type: Article

References (16)

1. Bombardelli, E.; Morazorri, P. Fitoterapia 1996, L67, 483.
2. Sda, H.; Li, Y.; Murakami, T.; Ninomiya, K.; Yoshikawa, M. Biol. Pharm. Bull. 1997, 20, 1092.
3. Yoshikawa, M.; Murakami, T.; Yamahara, Y.; Matsuda, H. Chem. Pharm. Bull. 1998, 46, 1764.
4. Yoshikawa, M.; Murakami, T.; Yamahara, J.; Matsuda, H.; Yamashina, J.; Murakami, N.; Kitagawa, I. Chem. Pharm. Bull. 1996, 44, 1454.
5. Wei, F.; Ma, S.C.; Ma, L.Y.; But, P.H.; Lin, R.C.; Khan, I.A. J. Nat. Prod. 2004, 67, 650.
6. Wei, F.; Ma, L.Y.; Ma, S.C.; Lin, R.C. Chinese Chem. Letts. 2002, 13, 59.
7. Griffini, A. Fitoterapia 1997, XVIII, 520.
8. Pharmacopoeia Commission of P. R. C., Pharamacopoeia of the People's Republic of China. (Vol. I, English edition 1997); Chemical Industry Press: Beijing, 1997; 207.
9. Yang, X.W.; Zhao, J.; Cui, Y.Y.; Liu, X.H.; Chao, M.M.; Hattori, M.; Zhang, L.H. J. Nat. Prod. 1999, 62, 1510.
10. Cesare, R.S. Pharmacol. Res. 2001, 44, 183.
11. Matsuda, H.; Li, Y.; Murakami, T.; Ninomiya, K.; Yoshikawa, M. Biol. Pharm. Bull. 1997, 20, 1092.
12. Matsuda, H.; Li, Y.; Murakami, T.; Ninomiya, K.; Araki, N.; Yoshikawa, M.; Yamahara, J. Bioorg. Med. Chem. Lett. 1997, 7, 1611.
13. Konoshima, T. J. Nat. Prod. 1986, 49, 650.
14. Wei, F.; Ma, L.Y.; Jin, W.T.; Ma, S.C.; Han, G.Z.; Khan, I.A.; Lin, R.C. Chem. Pharm. Bull. 2004, in press.
15. Pietta, P. J. Chromatogr. 1989, 478, 259.
16. Hubner, G.; Wray, V.; Nahrstedt, A. Planta Medica 1999, 65, 635.