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Volumn 15, Issue 6, 2005, Pages 1725-1729

Synthesis and fluorescence studies of thiazole orange tethered onto oligonucleotide: Development of a self-contained DNA biosensor on a fiber optic surface

Author keywords

Biosensor; DNA; Hybridization; Optical fiber; Thiazole orange

Indexed keywords

COMPLEMENTARY DNA; DNA HYBRID; ETHYLENE GLYCOL; OLIGONUCLEOTIDE; THIAZOLE ORANGE;

EID: 14644394836     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.01.030     Document Type: Article
Times cited : (45)

References (27)
  • 24
    • 85030807528 scopus 로고    scopus 로고
    • note
    • 4OH for 3 h, lyophilized, and redissolved in water. An estimate of the efficiency of the final coupling step could be determined by comparing the ratio of the free oligonucleotide (failed coupling product) to the conjugated oligonucleotide, as could be quantified by the reverse phase HPLC isolation procedure
  • 27
    • 85030809551 scopus 로고    scopus 로고
    • note
    • Sample (0.5 uM in 1 × PBS) delivery time 30 s, hybridization time 5 min, washing time 2 min at 1 mL/min with 1 × PBS. The experiment was run at 30°C


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.