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85030807528
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note
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4OH for 3 h, lyophilized, and redissolved in water. An estimate of the efficiency of the final coupling step could be determined by comparing the ratio of the free oligonucleotide (failed coupling product) to the conjugated oligonucleotide, as could be quantified by the reverse phase HPLC isolation procedure
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25
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0029347976
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27
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85030809551
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note
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Sample (0.5 uM in 1 × PBS) delivery time 30 s, hybridization time 5 min, washing time 2 min at 1 mL/min with 1 × PBS. The experiment was run at 30°C
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