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Volumn 277, Issue 5326, 1997, Pages 681-683

Failure of parturition in mice lacking the prostaglandin F receptor

Author keywords

[No Author keywords available]

Indexed keywords

OXYTOCIN; PROGESTERONE; PROSTAGLANDIN F2 ALPHA; PROSTAGLANDIN RECEPTOR;

EID: 14444268088     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5326.681     Document Type: Article
Times cited : (544)

References (26)
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    • note
    • 2-terminus of β-galactosidase. The MC1-herpes simplex virus thymidine kinase (HSV-TK) gene was inserted into the Spe I site at the 3′ end of the homologous region. Two lines of resultant embryonic stem (E14-1) cells injected into C57BL/6 blastocysts gave rise to chimeric offspring, which in turn were mated with C57BL/6 females.
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    • 14444272097 scopus 로고    scopus 로고
    • note
    • Ovaries or their sections (10 μm thick) were fixed with glutaraldehyde for 1 hour and stained with X-Gal (5-bromo-4-chloro-3-indoxyl-β-D-galactopyranoside) at 30°C. Sections were then counterstained with eosin.
  • 5
    • 14444276101 scopus 로고    scopus 로고
    • note
    • Gonadotropin-synchronized ovary was subjected to RNA isolation for Northern blot analysis or crude membrane preparation for binding assay. A Pst I-Bgl II fragment [690 base pairs (bp)] of FP cDNA (2) was used as an exon 2-specific probe.
  • 6
    • 0015491827 scopus 로고
    • J. A. McCracken et al., Nature New Biol. 238, 129 (1972); M. P. Moeljono, F. W. Bazer, W. W. Thatcher, Prostaglandins 14, 543 (1976).
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    • (1995) Nature , vol.378 , pp. 406
    • Dinchuk, J.E.1
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    • note
    • Animals were maintained at 23°C under a 12-hour light cycle. The estrous cycle was monitored by vaginal smears. Virgin females (9 to 12 weeks of age) housed overnight with males were checked the following morning for vaginal plugs, and their body weights were monitored. Plug day was counted as day 1.
  • 13
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    • D. McNellis, J. Challis, P. MacDonald, P. Nathanielsz, J. Roberts, Eds. Perinatology Press, Ithaca, NY
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    • (1988) The Onset of Labor: Cellular and Integrative Mechanisms , pp. 87-123
    • Soloff, M.S.1
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    • 2α or OT (Sigma) was administered intravenously from the tail vein. The pressure curve was taken for 10 min immediately before and immediately after the drug injection, and the contraction was quantified as the area under each curve.
    • (1981) Folia Pharmacol. Jpn. , vol.78 , pp. 231
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    • note
    • Northern blot analysis for OT receptor mRNA was performed with a PCR-amplified 700-bp fragment of mouse cDNA corresponding to the coding region from the second to the sixth putative transmembrane regions as a probe. Uterine expression of OT was analyzed by reverse transcriptase PCR (RT-PCR) with primers specific for mouse OT. Three independent experiments were performed for each analysis.
  • 19
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    • note
    • Blood was drawn from the abdominal aorta. Plasma concentrations of estradiol and progesterone were measured in duplicates by radioimmunoassay (Diagnostic Products, Los Angeles, CA). Statistical significance was examined by the Student's t test.
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    • K. Nishimori et al., Proc. Natl. Acad. Sci. U.S.A. 93, 11699 (1996); W. S. Young III et al., J. Neuroendocrinol. 8, 847 (1996).
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    • note
    • We thank K. Ishikawa, Y. Kataoka, and F. Ueno for technical assistance and T. Endo for helpful discussions. Supported by Grants-in-aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan and by grants from the Mochida Memorial Foundation for Medical and Pharmaceutical Research, the Sankyoh Life Science Research Foundation, the Katoh Memorial Foundation, and the Uehara Memorial Foundation.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.