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14444267839
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note
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2-terminus of β-galactosidase. The MC1-herpes simplex virus thymidine kinase (HSV-TK) gene was inserted into the Spe I site at the 3′ end of the homologous region. Two lines of resultant embryonic stem (E14-1) cells injected into C57BL/6 blastocysts gave rise to chimeric offspring, which in turn were mated with C57BL/6 females.
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4
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14444272097
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note
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Ovaries or their sections (10 μm thick) were fixed with glutaraldehyde for 1 hour and stained with X-Gal (5-bromo-4-chloro-3-indoxyl-β-D-galactopyranoside) at 30°C. Sections were then counterstained with eosin.
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5
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14444276101
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note
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Gonadotropin-synchronized ovary was subjected to RNA isolation for Northern blot analysis or crude membrane preparation for binding assay. A Pst I-Bgl II fragment [690 base pairs (bp)] of FP cDNA (2) was used as an exon 2-specific probe.
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6
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0015491827
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J. A. McCracken et al., Nature New Biol. 238, 129 (1972); M. P. Moeljono, F. W. Bazer, W. W. Thatcher, Prostaglandins 14, 543 (1976).
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14444267589
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note
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Animals were maintained at 23°C under a 12-hour light cycle. The estrous cycle was monitored by vaginal smears. Virgin females (9 to 12 weeks of age) housed overnight with males were checked the following morning for vaginal plugs, and their body weights were monitored. Plug day was counted as day 1.
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12
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0026550576
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T. Kimura, O. Tanizawa, K. Mori, M. J. Brownstein, H. Okayama, Nature 356, 526 (1992).
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D. McNellis, J. Challis, P. MacDonald, P. Nathanielsz, J. Roberts, Eds. Perinatology Press, Ithaca, NY
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M. S. Soloff, in The Onset of Labor: Cellular and Integrative Mechanisms, D. McNellis, J. Challis, P. MacDonald, P. Nathanielsz, J. Roberts, Eds. (Perinatology Press, Ithaca, NY, 1988), pp. 87-123.
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Soloff, M.S.1
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0019778864
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2α or OT (Sigma) was administered intravenously from the tail vein. The pressure curve was taken for 10 min immediately before and immediately after the drug injection, and the contraction was quantified as the area under each curve.
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Matsumoto, K.1
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14444280410
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note
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Northern blot analysis for OT receptor mRNA was performed with a PCR-amplified 700-bp fragment of mouse cDNA corresponding to the coding region from the second to the sixth putative transmembrane regions as a probe. Uterine expression of OT was analyzed by reverse transcriptase PCR (RT-PCR) with primers specific for mouse OT. Three independent experiments were performed for each analysis.
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16
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0026680513
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D. L. Lefebvre, A. Giaid, H. Bennett, R. Larivière, H. H. Zingg, Science 256, 1553 (1992).
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Fuchs, A.R.1
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19
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14444274146
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Blood was drawn from the abdominal aorta. Plasma concentrations of estradiol and progesterone were measured in duplicates by radioimmunoassay (Diagnostic Products, Los Angeles, CA). Statistical significance was examined by the Student's t test.
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21
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0017165935
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E. W. Horton and N. L. Poyser, Physiol. Rev. 56, 595 (1976); M. Alexandrova and M. S. Soloff, Endocrinology 106, 739 (1980).
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Young III, W.S.1
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14444283911
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note
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We thank K. Ishikawa, Y. Kataoka, and F. Ueno for technical assistance and T. Endo for helpful discussions. Supported by Grants-in-aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan and by grants from the Mochida Memorial Foundation for Medical and Pharmaceutical Research, the Sankyoh Life Science Research Foundation, the Katoh Memorial Foundation, and the Uehara Memorial Foundation.
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