PCR amplification of RoTat 1.2 VSG gene in Trypanosoma evansi isolates in Kenya

Veterinary Parasitology

Volumn 120, Issue 1-2, 2004, Pages 23-33

  Ngaira, J M ^a,b   Njagi, E N M ^b   Ngeranwa, J J N ^b   Olembo, N K ^c  

^a Kenyatta University ^*  (Kenya)

^b KENYATTA UNIVERSITY   (Kenya)

^c UNIVERSITY OF NAIROBI   (Kenya)

Author keywords

CATT T. evansi; Kenya; RoTat 1.2 VSG gene; Trypanosoma evansi

Indexed keywords

EPITOPE; PARASITE ANTIGEN; PROTOZOAL DNA; PROTOZOON ANTIBODY; ROTAT 1.2 VARIANT SURFACE GLYCOPROTEIN; UNCLASSIFIED DRUG; VARIANT SURFACE GLYCOPROTEIN;

ANTIBODY DETECTION; ARTICLE; CAMEL; CONTROLLED STUDY; DNA PURIFICATION; GENE AMPLIFICATION; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC ANALYSIS; IMMUNE RESPONSE; KENYA; NONHUMAN; PARASITE ISOLATION; POLYMERASE CHAIN REACTION; PROTOZOAL GENETICS; TRYPANOSOMA; TRYPANOSOMA EVANSI; WESTERN BLOTTING;

CAMELIDAE; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA EVANSI;

EID: 1442277182     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2003.12.007     Document Type: Article

References (29)

1. Artama W.T., Agey M.W., Donelson J.E. DNA comparisons of Trypanosoma evansi (Indonesia) and Trypanosoma brucei spp. Parasitology. 104:1992;67-74.
2. Bajyana Songa E., Hamers R. A card agglutination test (CATT) for veterinary use based on an early VAT RoTat 1.2 of Trypanosoma evansi. Ann. Soc. Belg. Med. Trop. 68:1988;233-240.
3. Boid R. Isoenzyme characterisation of 15 stocks of Trypanosoma evansi isolated from camels in the Sudan. Trop. Med. Parasitol. 39:1988;45-50.
4. Borst P., Fase-Fowler F., Gibson W.C. Kinetoplast DNA of Trypanosoma evansi. Mol. Biochem. Parasitol. 23:1987;31-38.
5. Burnette W.N. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112:1981;195-203.
6. Büscher P., Lejon V., Magnus E., Van Meirvenne N. Improved latex agglutination test for detection of antibodies in serum and cerebrospinal fluid of Trypanosoma brucei gambiense infected patients. Acta Trop. 73:1999;11-20.
7. Claes, F., 2003. Characterization of pathogenic Trypanosoma spp. How does Trypanosoma equiperdum fit into the Trypanozoon group? Doctoraatsproefschrift Nr. 565 aan de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen van de Katholieke Universiteit Leuven.
8. Claes, F., 2003. Expression of RoTat 1.2 crossreactive variable antigen type in T. evansi and T. equiperdum. Ann. NY Acad. Sci., 174-179.
9. Davison H.C., Thrusfield M.V., Muharsini S., Husein A., Partoutomo S., Rae P.F., Masake R., Luckins A.G. Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infection of buffaloes in Indonesia. Epidemiol. Infect. 123:1999;149-155.
10. Dukes P., Gibson W.C., Gashumba J.K., Hudson K.M., Bromidge T.S., Kaukus A., Asonganyi T., Magnus E. Absence of the LiTat 1.3 (CATT antigen) gene in Trypanosoma brucei gambiense stocks from Cameroon. Acta Trop. 51:1992;123-134.
11. Elsaid H.M., Nantulya V.M., Hilali M. Diagnosis of Trypanosoma evansi infection among Sudanese camels imported to Egypt using card agglutination test (CATT) and antigen detection latex agglutination test (Suratex). J. Protozool. Res. 8:1998;194-200.
12. Gibson W.C., Marshall T.F.C., Godfrey D.G. Numerical analysis of enzyme polymorphism: a new approach to the epidemiology and taxonomy of trypanosomes of the subgenus Trypanozoon. Adv. Parasitol. 18:1980;175-246.
13. Gibson W.C., Wilson A.J., Moloo S.K. Characterisation of Trypanosoma (Trypanozoon) evansi from camels in Kenya using isoenzyme electrophoresis. Res. Vet. Sci. 34:1983;114-118.
14. Hoare, C.A., 1972. The Trypanosomes of Mammals: A Zoological Monograph. Blackwell Scientific Publication, Oxford and Edinburgh, UK, pp. 555-592.
15. Jones T.W., McKinnell C.D. Antigenic variation in Trypanosoma evansi: a comparison of the predominant variable antigen type repertoire of stocks from Sudan. Trop. Med. Parasitol. 36:1985;205-209.
16. Jones T.W., McKinnell C.D. Antigenic variation in Trypanosoma evansi: variable antigen type development in mice, sheep and goats. Trop. Med. Parasitol. 36:1985;53-57.
17. 4. Nat. London. 227:1970;680-685.
18. Lanham S.M., Godfrey D.G. Isolation of salivarian trypanosomes from man and other mammals using DEAE cellulose. Exp. Parasitol. 28:1970;521-534.
19. Lun Z.R., Brun R., Gibson W. Kinetoplast DNA and molecular karyotypes of Trypanosoma evansi and Trypanosoma equiperdum from China. Mol. Biochem. Parasitol. 50:1992;189-196.
20. Masiga D.K., Gibson W.C. Specific probes for Trypanosoma (Trypanozoon) evansi based on kinetoplast DNA minicircles. Mol. Biochem. Parasitol. 40:1990;279-284.
21. Mathieu-Daude F., Tibayrenc M. Isozyme variability of Trypanosoma brucei sl.: genetic, taxonomic and epidemiological significance. Exp. Parasitol. 78:1994;1-19.
22. Ngaira J.M., Bett B., Karanja S.M., Njagi E.N.M. Evaluation of antigen and antibody rapid detection tests for Trypanosoma evansi infection in camels in Kenya. Vet. Parasitol. 114:2003;131-141.
23. Olaho-Mukani W., Mukunza F., Kimani J.K., Njoka P.K., Walubengo J. Evaluation of the in vitro transformation technique to distinguish Trypanosoma evansi from cyclically transmitted Trypanozoon stocks. Trop. Med. Parasitol. 44:1993;108-110.
24. Songa E.B., Paindavoine P., Wittouck E., Viseshakul N., Muldermans S., Steinert M., Hamers R. Evidence for kinetoplast and nuclear DNA homogeneity in Trypanosoma evansi isolates. Mol. Biochem. Parasitol. 43:1990;167-179.
25. Urakawa T., Verloo D., Moens L., Büscher P., Majiwa P.A.O. Trypanosoma evansi: cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat 1.2. Exp. Parasitol. 99:2001;181-189.
26. Van der Ploeg L.H.T., Cornelissen A.W.C.A., Michels P.A.M., Borst P. Chromosome rearrangements in Trypanosoma brucei. Cell. 39:1982;213-221.
27. Ventura R.M., Takeda G.F., Silva R.A.M.S., Nunes V.L.B., Buck G.A., Teixeira M.M.G. Genetic relatedness among Trypanosoma evansi stocks by random amplification of polymorphic DNA and evaluation of a synapomorphic DNA fragment for species-specific diagnosis. Int. J. Parasitol. 32:2002;53-63.
28. Verloo D., Magnus E., Büscher P. General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin. Vet. Parasitol. 97:2001;183-189.
29. Zweygarth E., Kaminsky R. In vitro differentiation between Trypanosoma brucei brucei and T. b. evansi. Trop. Med. Parasitol. 40:1989;115-116.