Molecular diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor in formalin-fixed paraffin-embedded tissues by RT-PCR and fluorescence in situ hybridization

Diagnostic Molecular Pathology

Volumn 14, Issue 1, 2005, Pages 23-28

  Qian, Xiang ^a   Jin, Long ^a   Shearer, Brandon M ^a   Ketterling, Rhett P ^a   Jalal, Syed M ^a   Lloyd, Ricardo V ^a,b  

^a MAYO CLINIC   (United States)

^b Dept of Lab Medicine and Pathology ^*  (United States)

Author keywords

Ewing's sarcoma; FISH; Paraffin section; RT PCR

Indexed keywords

FORMALDEHYDE; PARAFFIN; PHOSPHOGLYCERATE KINASE;

ARTICLE; DIAGNOSTIC ACCURACY; DNA HYBRIDIZATION; DNA SEQUENCE; EWING SARCOMA; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE FUSION; GENE TRANSLOCATION; GENETIC TRANSCRIPTION; HUMAN; HUMAN TISSUE; INTERPHASE; NEUROECTODERM TUMOR; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS;

BASE SEQUENCE; CHROMOSOMES, HUMAN, PAIR 11; CHROMOSOMES, HUMAN, PAIR 21; CHROMOSOMES, HUMAN, PAIR 22; DNA PRIMERS; DNA, NEOPLASM; FORMALDEHYDE; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INTERPHASE; NEUROECTODERMAL TUMORS, PRIMITIVE, PERIPHERAL; ONCOGENE PROTEINS, FUSION; PARAFFIN EMBEDDING; PROTO-ONCOGENE PROTEIN C-FLI-1; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SARCOMA, EWING'S; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION; TRANSCRIPTION FACTORS; TRANSLOCATION, GENETIC;

EID: 14144253827     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.pdm.0000140192.27878.97     Document Type: Article

References (19)

1. Weiss SW. Enzinger and Weiss's soft tissue tumors / Sharon W. Weiss, John R. Goldblum (4th ed.). St. Louis: Mosby, 2001.
2. Burchill SA. Ewing's sarcoma: diagnostic, prognostic, and therapeutic implications of molecular abnormalities. J Clin Pathol. 2003;56:96-102.
3. Hill DA, Pfeifer JD, Marley EF, et al. WT1 staining reliably differentiates desmoplastic small round cell tumor from Ewing's sarcoma/primitive neuroectodermal tumor. An immunohistochemical and molecular diagnostic study. Am J Clin Pathol. 2000;114:345-353.
4. Riley RD, Burchill SA, Abrams KR, et al. A systematic review of molecular and biological markers in tumours of the Ewing's sarcoma family. Eur J Cancer. 2003;39:19-30.
5. Dagher R, Pham TA, Sorbara L, et al. Molecular confirmation of Ewing's sarcoma. J Pediatr Hematol Oncol. 2001;23:221-224.
6. Kumar S, Pack S, Kumar D, et al. Detection of EWS-FLI-1 fusion in Ewing's sarcoma/peripheral primitive neuroectodermal tumor by fluorescence in situ hybridization using formalin-fixed paraffin-embedded tissue. Hum Pathol. 1999;30:324-330.
7. Taylor C, Patel K, Jones T, et al. Diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumour based on the detection of t(11;22) using fluorescence in situ hybridization. Br J Cancer. 1993;67:128-133.
8. Adams V, Hany MA, Schmid M, et al. Detection of t(11;22)(q24;q12) translocation breakpoint in paraffin-embedded tissue of the Ewing's sarcoma family by nested reverse transcription-polymerase chain reaction. Diagn Mol Pathol. 1996;5:107-113.
9. Monforte-Munoz H, Lopez-Terrada D, Affendie H, et al. Documentation of EWS gene rearrangements by fluorescence in-situ hybridization (FISH) in frozen sections of Ewing's sarcoma-peripheral primitive neuroectodermal tumor. Am J Surg Pathol. 1999;23:309-315.
10. Meier VS, Kuhne T, Jundt G, et al. Molecular diagnosis of Ewing's tumors: improved detection of EWS-FLI-1 and EWS-ERG chimeric transcripts and rapid determination of exon combinations. Diagn Mol Pathol. 1998;7:29-35.
11. Sorensen PH, Liu XF, Delattre O, et al. Reverse transcription PCR amplification of EWS/FLI-1 fusion transcripts as a diagnostic test for peripheral primitive neuroectodermal tumors of childhood. Diagn Mol Pathol. 1993;2:147-157.
12. Shing DC, McMullan DJ, Roberts P, et al. FUS/ERG gene fusions in Ewing's tumors. Cancer Res. 2003;63:4568-4576.
13. Zoubek A, Dockhorn-Dworniczak B, Delattre O, et al. Does expression of different EWS chimeric transcripts define clinically distinct risk groups of Ewing tumor patients? J Clin Oncol. 1996;14:1245-1251.
14. de Alava E, Kawai A, Healey JH, et al. EWS-FLI-1 fusion transcript structure is an independent determinant of prognosis in Ewing's sarcoma. J Clin Oncol. 1998;16:1248-1255.
15. de Alava E, Lozano MD, Patino A, et al. Ewing's family tumors: potential prognostic value of reverse-transcriptase polymerase chain reaction detection of minimal residual disease in peripheral blood samples. Diagn Mol Pathol. 1998;7:152-157.
16. de Alava E, Panizo A, Antonescu CR, et al. Association of EWS-FLI-1 type I fusion with lower proliferative rate in Ewing's sarcoma. Am J Pathol. 2000;156:849-855.
17. Lae ME, Roche PC, Jin L, et al. Desmoplastic small round cell tumor: a clinicopathologic, immunohistochemical, and molecular study of 32 tumors. Am J Surg Pathol. 2002;26:823-835.
18. Jin L, Majerus J, Oliveira A, et al. Detection of fusion gene transcripts in fresh-frozen and formalin fixed paraffin-embedded tissue sections of soft tissue sarcomas after laser capture microdissection and RT-PCR. Diagn Mol Pathol. 2003;12:224-230.
19. Kovar H, Jugovic D, Melot T, et al. Cryptic exons as a source of increased diversity of Ewing tumor-associated EWS-FLI1 chimeric products. Genomics. 1999;60:371-374.