Specific cross-linking of Lys233 and Cys235 in the mu opioid receptor by a reporter affinity label

Biochemistry

Volumn 44, Issue 7, 2005, Pages 2271-2275

  Zhang, Yan ^a   McCurdy, Christopher R ^a   Metzger, Thomas G ^a   Portoghese, Philip S ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDES; AMINO ACIDS; CELLS; CHEMICAL BONDS; CROSSLINKING; FLUORESCENCE; NAPHTHALENE;

COVALENT BONDING; CYSTEINE; CYTOMETRY; FLUOROGENIC NAPHTHALENE DIALDEHYDE MOIETY;

CYTOLOGY;

ALDEHYDE; CYSTEINE; DELTA OPIATE RECEPTOR; ISOINDOLE DERIVATIVE; KAPPA OPIATE RECEPTOR; LYSINE; MU OPIATE RECEPTOR; NAPHTHALENE DERIVATIVE; NAPHTHALENE DIALDEHYDE; OPIATE RECEPTOR; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CHEMICAL BINDING; CHEMICAL REACTION KINETICS; CROSS LINKING; FLOW CYTOMETRY; FLUORESCENCE; MOLECULAR RECOGNITION; PRIORITY JOURNAL; RECEPTOR BINDING; SITE DIRECTED MUTAGENESIS;

ARGININE; BINDING, COMPETITIVE; CELL LINE; CROSS-LINKING REAGENTS; CYSTEINE; FLUOROMETRY; GENES, REPORTER; HUMANS; KINETICS; LYSINE; MUTAGENESIS, SITE-DIRECTED; NAPHTHALENES; PHOTOAFFINITY LABELS; PROTEIN BINDING; RECEPTORS, OPIOID, DELTA; RECEPTORS, OPIOID, KAPPA; RECEPTORS, OPIOID, MU; SERINE;

EID: 14044269530     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi048049v     Document Type: Article

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23. max} = -kt Here [RL*] max may be substituted by the highest fluorescence intensity during a certain time course, while [RL*] may be the fluorescence intensity at a certain point of time.
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25. max values are 367 ± 163 pM and 139 ± 24 pM, respectively.
26. An analogue bearing a naphthalene in the place of the naphthalenedialdehyde moiety in NNA exhibited wash resistance in smooth muscle preparations (mouse vas deferens and guinea pig ileum) (unpublished data). This suggests that very strong noncovalent binding of NNA with the mutant receptors was responsible for the observed wash resistance.
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