Down-regulation of connexin43 in early myocardial ischemia and protective effect by ischemic preconditioning in rat hearts in vivo

Japanese Heart Journal

Volumn 45, Issue 6, 2004, Pages 1007-1019

  Hatanaka, Kazuhito ^a   Kawata, Hiroyuki ^b   Toyofuku, Toshihiko ^c   Yoshida, Ken Ichi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b NARA MEDICAL UNIVERSITY   (Japan)

^c OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Connexin 43; Degradation; Dephosphorylation; Ischemic preconditioning; Myocardial ischemia; Protein kinase C

Indexed keywords

CALCINEURIN; CHELERYTHRINE; CONNEXIN 43; CYCLOSPORIN A; ENZYME INHIBITOR; LYSOSOMAL INHIBITOR; LYSOSOME ENZYME; MESSENGER RNA; PROTEASOME; PROTEASOME INHIBITOR; UNCLASSIFIED DRUG; ALKALOID; BENZOPHENANTHRIDINE DERIVATIVE; PHENANTHRIDINE DERIVATIVE; PROTEIN KINASE C;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; DOWN REGULATION; ENZYME DEGRADATION; HEART INFARCTION; HEART MUSCLE ISCHEMIA; HEART MUSCLE REPERFUSION; HEART PROTECTION; IN VIVO STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DEPHOSPHORYLATION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; RAT; RAT STRAIN; ANIMAL; DRUG ANTAGONISM; DRUG EFFECT; GENETICS; HEART INFARCTION PREVENTION; IMMUNOHISTOCHEMISTRY; METABOLISM; NORTHERN BLOTTING; PHOSPHORYLATION; SPRAGUE DAWLEY RAT;

ALKALOIDS; ANIMALS; BENZOPHENANTHRIDINES; BLOTTING, NORTHERN; CONNEXIN 43; DOWN-REGULATION; IMMUNOHISTOCHEMISTRY; ISCHEMIC PRECONDITIONING, MYOCARDIAL; MALE; MYOCARDIAL ISCHEMIA; PHENANTHRIDINES; PHOSPHORYLATION; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN KINASE C; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER;

EID: 13844322129     PISSN: 00214868     EISSN: None     Source Type: Journal    
DOI: 10.1536/jhj.45.1007     Document Type: Article

References (25)

1. Saffitz JE, Laing JG, Yamada KA. Connexin expression and turnover; implications for cardiac excitability. Circ Res 2000; 86: 723-8. (Review)
2. Huang XD, Sandusky GE, Zipes DP. Heterogeneous loss of connexin43 protein in ischemic dog hearts. J Cardiovasc Electrophysiol 1999; 10: 79-91.
3. Matsushita T, Takamatsu T. Ischaemia-induced temporal expression of connexin43 in rat heart. Virchows Arch 1997; 431: 453-8.
4. Peters NS, Coromilas J, Severs NJ, Wit AL. Disturbed connexin43 gap junction distribution correlates with the location of reentrant circuits in the epicardial border zone of healing canine infarcts that cause ventricular tachycardia. Circulation 1997; 95: 988-96.
5. Lerner DL, Yamada KA, Schuessler RB, Saffitz JE. Accelerated onset and increased incidence of ventricular arrhythmias induced by ischemia in Cx43-deficient mice. Circulation 2000; 101: 547-52.
6. Beardslee MA, Lerner DL, Tadros PN, et al. Dephosphorylation and intracellular redistribution of ventricular connexin43 during electrical uncoupling induced by ischemia. Circ Res 2000; 87: 656-62.
7. Li WE, Nagy JI. Connexin43 phosphorylation state and intercellular communication in cultured astrocytes following hypoxia and protein phosphatase inhibition. Eur J Neurosci 2000; 12: 2644-50.
8. Beardslee MA, Laing JG, Beyer EC, Saffitz JE. Rapid turnover of connexin43 in the adult rat heart. Circ Res 1998; 83: 629-35.
9. Parratt J, Vegh A. Pronounced antiarrhythmic effects of ischemic preconditioning. Cardioscience 1994; 5: 9-18. (Review)
10. Kawata H, Yoshida K, Kawamoto A, et al. Ischemic preconditioning upregulates vascular endothelial growth factor mRNA expression and neovascularization via nuclear translocation of protein kinase C epsilon in the rat ischemic myocardium. Circ Res 2001; 88: 696-704.
11. Sasaki Y, Sakai R, Furuta S. Evaluation of repeated balloon inflation in angioplasty as a clinical model of ischemic preconditioning. Jpn Heart J 1995; 36: 719-28.
12. + channels in myocardial ischemia. Jpn Heart J 1997; 38: 297-315.
13. Schwanke U, Konietzka I, Duschin A, Li X, Schulz R, Heusch G. No ischemic preconditioning in heterozygous connexin43-deficient mice. Am J Physiol Heart Circ Physiol 2002; 283: H1740-2.
14. Miura T, Ohnuma Y, Kuno A, et al. Protective role of gap junctions in preconditioning against myocardial infarction. Am J Physiol Heart Circ Physiol 2004; 286: H214-21.
15. Schulz R, Gres P, Skyschally A, et al. Ischemic preconditioning preserves connexin43 phosphorylation during sustained ischemia in pig hearts in vivo. FASEB J 2003; 17: 1355-7.
16. Jain SK, Schuessler RB, Saffitz JE. Mechanism of delayed electrical uncoupling induced by ischemic preconditioning. Circ Res 2003; 92: 1138-44.
17. Squadrito F, Altavilla D, Squadrito G, et al. Cyclosporin-A reduces leukocyte accumulation and protects against myocardial ischemia reperfusion injury in rats. Eur J Pharmacol 1999; 364: 159-68.
18. Ohshita T, Nikawa T, Towatari T, Katunuma N. Effects of selective inhibition of cathepsin B and general inhibition of cysteine proteinases on lysosomal proteolysis in rat liver in vivo and in vitro. Eur J Biochem 1992; 209: 223-31.
19. Takaoka M, Itoh M, Hayashi S, Kuro T, Matsumura Y. Proteasome participates in the pathogenesis of ischemic acute renal failure in rats. Eur J Phamnacol 1999; 384: 43-6.
20. Fukuda S, Harada K, Kunimatsu M, Sakabe T, Yoshida K. Postischemic reperfusion induces α-fodrin proteolysis by m-calpain in the synaptosome and nucleus in rat brain. J Neurochem 1998; 70: 2526-32.
21. Gray EG, Whittaker VP. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J Anat 1962; 96: 79-88.
22. Shinohara K, Funabashi T, Mitsushima D, Kimura F. Effects of estrogen on the expression of connexin32 and connexin43 mRNAs in the suprachiasmatic nucleus of female rats. Neurosci Lett 2000; 286: 107-10.
23. Nagy JI, Li WE, Roy C, et al. Selective monoclonal antibody recognition and cellular localization of an unphosphorylated form of connexin43. Exp Cell Res 1997; 236: 127-36.
24. Yoshida K, Inui M, Harada K, et al. Reperfusion of rat heart after brief ischemia induces proteolysis of calspectin (nonerythroid spectrin or fodrin) by calpain. Circ Res 1995; 77: 603-10.
25. Li G, Whittaker P, Yao M, Kloner RA, Przyklenk K. The gap junction uncoupler heptanol abrogates infarct size reduction with preconditioning in mouse hearts. Cardiovasc Pathol 2002; 11: 158-65.