Generating oxidation-resistant variants of Bacillus kaustophilus leucine aminopeptidase by substitution of the critical methionine residues with leucine

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 86, Issue 4, 2005, Pages 355-362

  Chi, Meng Chun ^a   Chou, Wei Mou ^a   Wang, Chih Hsien ^a   Chen, Wenlung ^a   Hsu, Wen Hwei ^b   Lin, Long Liu ^a  

^a NATIONAL CHIAYI UNIVERSITY   (Taiwan)

^b NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Bacillus kaustophilus; Chemical oxidation; Directed mutagenesis; Leucine aminopeptidase; Methionine

Indexed keywords

CYTOSOL AMINOPEPTIDASE; ENZYME VARIANT; HYDROGEN PEROXIDE; LEUCINE; METHIONINE; NICKEL COMPLEX;

ENZYME; OXIDATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS; BACILLUS KAUSTOPHILUS; BACTERIAL CELL; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME ENGINEERING; ENZYME METABOLISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GENE MUTATION; GENE OVEREXPRESSION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SITE DIRECTED MUTAGENESIS; STATISTICAL SIGNIFICANCE; WILD TYPE;

AMINO ACID SUBSTITUTION; BACILLUS; ENZYME STABILITY; HYDROGEN PEROXIDE; LEUCINE; LEUCYL AMINOPEPTIDASE; METHIONINE; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; OXYGEN;

ESCHERICHIA COLI; GEOBACILLUS KAUSTOPHILUS;

EID: 13844316356     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10482-005-0518-5     Document Type: Article

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