Purification and characterization of baicalin-β-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge

Process Biochemistry

Volumn 40, Issue 5, 2005, Pages 1911-1915

  Zhang, Chunzhi ^a,b   Zhang, Yufei ^b   Chen, Jiping ^a   Liang, Xinmiao ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b DALIAN POLYTECHNIC UNIVERSITY   (China)

Author keywords

Baicalin D glucuronidase; Characterization; Purification; Scutellaria viscidula Bge

Indexed keywords

ACTIVATION ANALYSIS; BACTERIA; CHEMICAL BONDS; ELECTROPHORESIS; ENZYME KINETICS; HYDROLYSIS; PH EFFECTS; PURIFICATION; THERMAL EFFECTS;

BAICALEIN; ENZYME ACTIVITY; HYDROLYZES; PLANT ROOTS;

ENZYMES;

SCUTELLARIA;

EID: 13844296831     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2004.07.003     Document Type: Article

References (20)

1. M. Kubo, Y. Kimura, T. Odani, T. Tani, and K. Namba Studies on Scutellariae radix. Part II: the antibacterial substance Planta Med 43 1981 194 201
2. C.A. Austin, S. Patel, K. Ono, H. Nakane, and L.M. Fisher Site-specific DNA cleavage by mammalian DNA topoisomerase II induced by novel flavone and catechin derivatives Biochem J 282 1992 883 889
3. Y.C. Shen, W.F. Chiou, Y.C. Chou, and C.F. Chen Mechanisms in mediating the anti-inflammatory effects of baicalin and baicalein in human leukocytes Eur J Pharmacol 465 2003 171 181
4. J.J. Liu, T.S. Huang, W.F. Cheng, and F.J. Lu Baicalein and baicalin are potent inhibitors of angiogenesis: inhibition of endothelial cell proliferation, migration and differentiation Int J Cancer 106 2003 559 565
5. S. Ikemoto, K. Sugimura, N. Yoshida, R. Yasumoto, S. Wada, and K. Yamamoto Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines Urology 55 2000 951 955
6. J.A. Wu, A.S. Attele, L. Zhang, and C.S. Yuan Anti-HIV activity of medicinal herbs: usage and potential development Am J Chin Med 29 2001 69 81
7. D.E. Shieh, L.T. Liu, and C.C. Lin Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin Anticancer Res 20 2000 2861 2865
8. M.Y. Lai, S.L. Hsiu, C.C. Chen, Y.C. Hou, and P.D. Chao Urinary pharmacokinetics of baicalein, wogonin and their glycosides after oral administration of Scutellariae radix in humans Biol Pharm Bull 26 2003 79 83
9. M.Y. Lai, S.L. Hsiu, S.Y. Tsai, Y.C. Hou, and P.D. Chao Comparison of metabolic pharmacokinetics of baicalin and baicalein in rats J Pharm Pharmacol 55 2003 205 209
10. H.Y. Wang, L.H. Xiao, L. Liu, and S.X. Xu Studies on chemical constituents of the roots of Scutellaria viscidula Bge Shenyang Yao ke Da Xue Xue Bao. 20 2003 339 348 (Chinese)
11. 2 from human intestinal bacteria Biol Pharm Bull 23 2000 1481 1485
12. T. Akao, T. Akao, and K. Kobashi Glycyrrhizin β-d-glucuronidase of Eubacterium sp. from human intestinal flora Chem Pharm Bull 35 1987 705 710
13. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
14. K. Weber, J.R. Pringle, and M. Osborn Measurement of molecular weights by electrophoresis on SDS-acrylamide gel method Enzymology 26 1971 3 27
15. S. Morimoto, T. Harioka, and Y. Shoyama Purification and characterization of flavone-specific β-glucuronidase from callus cultures of Scutellaria baicalensis Georgi Planta 195 1995 535 540
16. C.Z. Zhang, H.S. Yu, Y.M. Bao, L.J. An, and F.X. Jin Purification and characterization of ginsenoside-β-glucosidase from ginseng Chem Pharm Bull 49 2001 795 798
17. G.A. Levvy Baicalinase a plant β-glucuronidase Biochem J 58 1954 462 469
18. F. Ikegami, K. Matsunae, M. Hisamitsu, T. Kurihara, T. Yamamoto, and I. Murakoshi Purification and properties of a plant beta-d-glucuron idase form Scutellaria root Biol Pharm Bull 18 1995 1531 1534
19. K. Sasaki, F. Taura, Y. Shoyama, and S. Morimoto Molecular characterization of a novel β-glucuronidase from Scutellaria baicalensis Georgi J Biol Chem 275 2000 27466 27472
20. S. Morimoto, N. Tateishi, T. Matsuda, H. Tanaka, and F. Taura Novel hydrogen peroxide metabolism in suspension cells of Scutellaria baicalensis Georgi J Biol Chem 273 1998 12606 12611