Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein

FEBS Letters

Volumn 579, Issue 5, 2005, Pages 1008-1014

  Stepanyuk, Galina A ^a   Golz, Stefan ^b   Markova, Svetlana V ^a,c   Frank, Ludmila A ^a   Lee, John ^c   Vysotski, Eugene S ^a,c  

^a INSTITUTE OF BIOPHYSICS   (Russian Federation)

^b BAYER PHARMA AG   (Germany)

^c UNIVERSITY OF GEORGIA   (United States)

Author keywords

Calcium; Coelenterazine; Fluorescence spectrum; Mammalian expression; Reporter protein

Indexed keywords

2 AMINO 2 METHYL 3 (2 TRIFLUOROMETHYL 5 BENZIMIDAZOLYL)PROPIONIC ACID; AEQUORIN; AMIDE; OBELIN; PHENYL GROUP; PHOTOPROTEIN; PROTEIN DERIVATIVE; TRYPSIN; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BIOLOGICAL MONITORING; BIOLUMINESCENCE; CHEMICAL MODIFICATION; COLORIMETRY; CONTROLLED STUDY; GENE MUTATION; HYDROGEN BOND; MAMMAL CELL; MOLECULAR BIOLOGY; MOLECULAR INTERACTION; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; SPECTRAL SENSITIVITY; SUBSTITUTION REACTION; WAVEFORM;

EID: 13844278292     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2005.01.004     Document Type: Article

References (49)

1. J.G. Morin Coelenterate bioluminescence L. Muscatine H.M. Lenhoff Coelenterate Biology: Reviews and New Perspectives 1974 Academic Press New York 397 438
2. O. Shimomura, F.H. Johnson, and Y. Saiga Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea J. Cell. Comp. Physiol. 59 1962 223 239
3. V.S. Bondar, K.P. Trofimov, and E.S. Vysotski Physicochemical properties of a photoprotein from the hydroid polyp Obelia longissima Biochemistry-Moscow 57 1992 1481 1490
4. D. Prasher, R.O. McCann, and M.J. Cormier Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium-binding protein Biochem. Biophys. Res. Commun. 126 1985 1259 1268
5. S. Inouye, M. Noguchi, Y. Sakaki, Y. Takagi, T. Miyata, S. Iwanaga, T. Miyata, and F.I. Tsuji Cloning and sequence analysis of cDNA for the luminescent protein aequorin Proc. Natl. Acad. Sci. USA 82 1985 3154 3158
6. D.C. Prasher, R.O. McCann, M. Longiaru, and M.J. Cormier Sequence comparisons of complementary DNAs encoding aequorin isotypes Biochemistry 26 1987 1326 1332
7. 2+-activated photoprotein, clytin FEBS Lett. 315 1993 343 346
8. 2+-binding photoprotein, mitrocomin FEBS Lett. 333 1993 301 305
9. 2+-activated photoprotein obelin from the hydroid polyp Obelia longissima Dokl. Akad. Nauk 326 1992 911 913 (in Russian)
10. 2+-activated photoprotein obelin from the hydroid polyp Obelia longissima Gene 153 1995 273 274
11. 2+-regulated photoproteins Biochemistry 41 2002 2227 2236
12. H. Charbonneau, K.A. Walsh, R.O. McCann, F.G. Prendergast, M.J. Cormier, and T.C. Vanaman Amino acid sequence of the calcium-dependent photoprotein aequorin Biochemistry 24 1985 6762 6771
13. 2+-binding photoproteins of the Hydrozoa Photochem. Photobiol. 62 1995 657 661
14. Y. Ohmiya, and T. Hirano Shining the light: the mechanism of the bioluminescence reaction of calcium-binding photoproteins Chem. Biol. 3 1996 337 347
15. O. Shimomura Cause of spectral variation in the luminescence of semisynthetic aequorins Biochem. J. 306 1995 537 543
16. 2+-regulated photoproteins: structural insight into the bioluminescence mechanism Acc. Chem. Res. 37 2004 405 415
17. J.F. Head, S. Inouye, K. Teranishi, and O. Shimomura The crystal structure of the photoprotein aequorin at 2.3 Å resolution Nature 405 2000 372 376
18. 2+-regulated photoprotein obelin at 1.7 Å resolution determined directly from its sulfur substructure Protein Sci. 11 2000 2085 2093
19. Z.-J. Liu, E.S. Vysotski, L. Deng, J. Lee, J. Rose, and B.-C. Wang Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine Biochem. Biophys. Res. Commun. 311 2003 433 439
20. L. Deng, E.S. Vysotski, Z.-J. Liu, S.V. Markova, N.P. Malikova, J. Lee, J. Rose, and B.-C. Wang Structural basis for the emission of violet bioluminescence from a W92F obelin mutant FEBS Lett. 506 2001 281 285
21. E.S. Vysotski, Z.-J. Liu, S.V. Markova, J.R. Blinks, L. Deng, L.A. Frank, M. Herko, N.P. Malikova, J.P. Rose, B.-C. Wang, and J. Lee Violet bioluminescence and fast kinetics from W92F obelin: structure-based proposals for the bioluminescence triggering and the identification of the emitting species Biochemistry 42 2003 6013 6024
22. J.W. Hastings, and Q.H. Gibson Intermediates in the bioluminescent oxidation of reduced flavin mononucleotide J. Biol. Chem. 238 1963 2537 2554
23. 2+-discharged photoprotein obelin Acta Crystallogr. D 60 2004 512 514
24. 2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence J. Biol. Chem. 279 2004 33647 33652
25. J. Nakajima-Shimada, H. Iida, F.I. Tsuji, and Y. Anraku Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system Proc. Natl. Acad. Sci. USA 88 1991 6878 6882
26. S. Saran, H. Nakao, M. Tasaka, H. Iida, F.I. Tsuji, V. Nanjundiah, and I. Takeuchi Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA FEBS Lett. 337 1994 43 47
27. V.J. Dupriez, K. Maes, E. Le Poul, E. Burgeon, and M. Detheux Aequorin-based functional assays for G-protein-coupled receptors, ion channels, and tyrosine kinase receptors Receptor Channel 8 2002 319 330
28. S.V. Markova, E.S. Vysotski, and J. Lee Obelin hyperexpression in Escherichia coli, purification and characterization J.F. Case P.J. Herring B.H. Robison S.H.D. Haddock L.J. Kricka P.E. Stanley Bioluminescence and Chemiluminescence 2000 2001 World Scientific Singapore 115 118
29. B.A. Illarionov, L.A. Frank, V.A. Illarionova, V.S. Bondar, E.S. Vysotski, and J.R. Blinks Recombinant obelin: cloning and expression of cDNA purification, and characterization as a calcium indicator Meth. Enzymol. 305 2000 223 249
30. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 2nd edn. 1989 Cold Spring Harbor Laboratory Press New York
31. T.A. Brown Essential Molecular Biology I: A Practical Approach Series 1990 IRL Press Oxford
32. 2+ concentrations in living cells Prog. Biophys. Mol. Biol. 40 1982 1 114
33. N. Solovyova, and A. Verkhratsky Monitoring of free calcium in the neuronal endoplasmic reticulum: an overview of modern approaches J. Neurosci. Meth. 122 2002 1 12
34. 2+] in the endoplasmic reticulum with aequorin Cell Calcium 32 2002 251 260
35. 3, and 40 years before Nat. Rev. Mol. Cell Biol. 4 2003 586 590
36. P.A. Iredale, and S.J. Hill Increases in intracellular calcium via activation of an endogenous P2-purinoceptor in cultured CHO-K1 cells Br. J. Pharmacol. 110 1993 1305 1310
37. A.C. Megson, J.M. Dickenson, A. Townsend-Nicholson, and S.J. Hill Synergy between the inositol phosphate responses to transfected human adenosine A1-receptors and constitutive P2-purinoceptors in CHO-K1 cells Br. J. Pharmacol. 115 1995 1415 1424
38. R.Y. Tsien The green fluorescent protein Annu. Rev. Biochem. 67 1998 509 544
39. J. Zhang, R.E. Campbell, A.Y. Ting, and R.Y. Tsien Creating new fluorescent probes for cell biology Nat. Rev. Mol. Cell Biol. 3 2002 906 918
40. T. Zimmermann, J. Rietdorf, and R. Pepperkok Spectral imaging and its applications in live cell microscopy FEBS Lett. 546 2003 87 92
41. N. Kajiyama, and E. Nakano Isolation and characterization of mutants of firefly luciferase which produce different colors of light Protein Eng. 4 1991 691 693
42. Y. Ohmiya, T. Hirano, and M. Ohashi The structural origin of the color differences in the bioluminescence of firefly luciferase FEBS Lett. 384 1996 83 86
43. B.R. Branchini, R.A. Magyar, M.H. Murtiashaw, S.M. Anderson, L.C. Helgerson, and M. Zimmer Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color Biochemistry 38 1999 13223 13230
44. V. Viviani, A. Uchida, N. Suenaga, M. Ryufuku, and Y. Ohmiya Thr226 is a key residue for bioluminescence spectra determination in beetle luciferases Biochem. Biophys. Res. Commun. 280 2001 1286 1291
45. B.R. Branchini, T.L. Southworth, M.H. Murtiashaw, R.A. Magyar, S.A. Gonzalez, M.C. Ruggiero, and J.G. Stroh An alternative mechanism of bioluminescence color determination in firefly luciferase Biochemistry 43 2004 7255 7262
46. N.P. Malikova, G.A. Stepanyuk, L.A. Frank, S.V. Markova, E.S. Vysotski, and J. Lee Spectral tuning of obelin bioluminescence by mutations of Trp92 FEBS Lett. 554 2003 184 188
47. H. Ohkuma, K. Abe, Y. Kosaka, and M. Maeda Detection of luciferase having two kinds of luminescent color based on optical filter procedure: application to an enzyme immunoassay Luminescence 15 2000 21 27
48. Y. Kitayama, T. Kondo, Y. Nakahira, H. Nishimura, Y. Ohmiya, and T. Oyama An in vivo dual-reporter system of cyanobacteria using two railroad-worm luciferases with different color emissions Plant Cell Physiol. 45 2004 109 113
49. Y. Nakajima, M. Ikeda, T. Kimura, S. Honma, Y. Ohmiya, and K. Honma Bidirectional role of orphan nuclear receptor RORalpha in clock gene transcriptions demonstrated by a novel reporter assay system FEBS Lett. 565 2004 122 126