Development, characterization and diagnostic application of a monoclonal antibody specific for a proteinase K resistant Lawsonia intracellularis antigen

Veterinary Microbiology

Volumn 105, Issue 3-4, 2005, Pages 199-206

  Boesen, Henriette T ^a   Jensen, Tim K ^a   Jungersen, Gregers ^a   Riber, Ulla ^a   Boye, Mette ^a   Møller, Kristian ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Lawsonia intracellularis; Lipopolysaccharide; Monoclonal antibodies

Indexed keywords

BACTERIAL ANTIGEN; IMMUNOGLOBULIN G2B; MONOCLONAL ANTIBODY; PROTEINASE K;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY SPECIFICITY; ARTICLE; BACTERIAL GROWTH; CELL CULTURE; CONTROLLED STUDY; DESULFOVIBRIONACEAE INFECTION; DIAGNOSTIC PROCEDURE; DIGESTION; ENTEROPATHY; HEAT; HISTOPATHOLOGY; HYBRIDOMA; IMMUNIZATION; IMMUNOASSAY; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; INTESTINE MUCOSA; LAWSONIA; LAWSONIA INTRACELLULARIS; MOLECULAR WEIGHT; MOLECULE; MOUSE; MYELOMA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPLEEN CELL; SWINE; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); LAWSONIA INTRACELLULARIS; SUS SCROFA;

EID: 13644252228     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2004.10.022     Document Type: Article

References (17)

1. M. Boye, T.K. Jensen, K. Møller, T.O. Leser, and S.E. Jorsal Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization Vet. Pathol. 35 1998 153 156
2. K. Frydendahl, T.K. Jensen, J.S. Andersen, M.S. Fredholm, and G. Evans Association between the porcine Escherichia coli F18 receptor genotype and phenotype and susceptibility to colonisation and postweaning diarrhoea caused by E. coli O138:F18 Vet. Microbiol. 93 2003 39 51
3. R.M.C. Guedes, and C.J. Gebhart Comparison of intestinal mucosa homogenate and pure culture of the homologous Lawsonia intracellularis isolate in reproducing proliferative enteropathy in swine Vet. Microbiol. 93 2003 159 166
4. R.M.C. Guedes, and C.J. Gebhart Preparation and characterization of polyclonal and monoclonal antibodies against Lawsonia intracellularis J. Vet. Diagn. Invest. 15 2003 438 446
5. P.J. Hitchcock, and T.M. Brown Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels J. Bacteriol. 154 1983 269 277
6. P.K. Holyoake, R.S. Cutler, I.W. Caple, and R.P. Monckton Enzyme-linked immunosorbent assay for measuring ileal symbiont intracellularis-specific immunoglobulin G response in sera of pigs J. Clin. Microbiol. 32 1994 1980 1985
7. T.K. Jensen, K. Møller, T.D. Leser, and S.E. Jorsal Comparison of histology, immunohistochemistry and polymerase chain reaction for detection of Lawsonia intracellularis in natural porcine proliferative enteropathy Eur. J. Vet. Pathol. 3 1997 115 123
8. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
9. G.H.K. Lawson, and C.J. Gebhart Proliferative enteropathy J. Comp. Pathol. 122 2000 77 100
10. G.H.K. Lawson, S. McOrist, S. Jasni, and R.A. Mackie Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro J. Clin. Microbiol. 31 1993 1136 1142
11. J. McCluskey, J. Hannigan, J.D. Harris, B. Wren, and D.G.E. Smith LsaA, an antigen involved in cell attachment and invasion, is expressed by Lawsonia intracellularis during infection in vitro and in vivo Infect. Immun. 70 2002 2899 2907
12. S. McOrist, R. Boid, and G.H.K. Lawson Antigenic analysis of Campylobacter species and an intracellular Campylobacter-like organism associated with porcine proliferative enteropathies Infect. Immun. 57 1989 957 962
13. S. McOrist, R. Boid, G.H.K. Lawson, and I. McConnell Monoclonal antibodies to intracellular campylobacter-like organisms of the porcine proliferative enteropathies Vet. Rec. 121 1987 421 422
14. S. McOrist, C.J. Gebhart, R. Boid, and S.M. Barns Characterization of Lawsonia intracellularis gen. nov., sp. nov., the obligately intracellular bacterium of porcine proliferative enteropathy Int. J. Syst. Bacteriol. 45 1995 820 825
15. C.M. Tsai, and C.E. Frasch A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels Anal. Biochem. 119 1982 115 119
16. O. Westphal, and K. Jann Bacterial Lipopolysaccharides R.L. Whistler Methods in Carbohydrate Chemistry 1965 Academic Press Inc. New York pp. 83-91
17. M.P. Woodward, W.W. Young Jr., and R.A. Bloodgood Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation J. Immunol. Methods 78 1985 143 153