Codon optimization of the BirA enzyme gene leads to higher expression and an improved efficiency of biotinylation of target proteins in mammalian cells

Journal of Biotechnology

Volumn 116, Issue 3, 2005, Pages 245-249

  Mechold, Undine ^a,b   Gilbert, Cristele ^a   Ogryzko, Vasily ^a  

^a CNRS UPR 9079 Oncogenese Differenciation et Transduction du Signal   (France)

^b INSTITUT PASTEUR   (France)

Author keywords

Biotinylation; BirA; Codon optimization; In vivo

Indexed keywords

BACTERIOLOGY; BIOTECHNOLOGY; CELLS; ENZYMES; MACROMOLECULES; OPTIMIZATION; PURIFICATION;

BIOTIN ACCEPTOR; BIOTINYLATION; CODONS; REAGENTS;

GENES;

ISOENZYME; PROTEIN BIRA; UNCLASSIFIED DRUG;

ARTICLE; BIOTINYLATION; BIRA GENE; CODON; CONTROLLED STUDY; GENE; GENE CONSTRUCT; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VIVO STUDY; MAMMAL CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN TARGETING;

BIOTINYLATION; CARBON-NITROGEN LIGASES; CELL LINE; CODON; ESCHERICHIA COLI PROTEINS; GENE TARGETING; GENETIC ENHANCEMENT; HUMANS; KIDNEY; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; TRANSCRIPTION FACTORS; TRANSFECTION;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 13544274323     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.12.003     Document Type: Article

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