Purification and characterization of catechol 1,2-dioxygenase of Pseudomonas fluorescens for degradation of 4-chlorobenzoic acid

Indian Journal of Biotechnology

Volumn 4, Issue 1, 2005, Pages 134-138

  Saxena, Preti ^a   Thakur, Indu Shekhar ^b  

^a G B PANT UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (India)

^b JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

4 chlorobenzoic acid; Catechol 1,2 dioxygenase; Chlorocatechol; DEAE cellulose; Gel filtration; SDS polyacrylamide gel electrophoresis

Indexed keywords

4 CHLOROBENZOIC ACID; CATECHOL 1,2 DIOXYGENASE; DIETHYLAMINOETHYL CELLULOSE;

ANIMAL CELL; ARTICLE; CELL FRACTIONATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; GEL FILTRATION CHROMATOGRAPHY; ION EXCHANGE; MOLECULAR WEIGHT; NONHUMAN; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PSEUDOMONAS FLUORESCENS; TEMPERATURE;

ANIMALIA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 13544258806     PISSN: 09725849     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Reineke W & Knackmuss H, Hybrid pathways for chlorobenzoate metabolism in Pseudomonas sp. B13 derivatives, J Bacteriol, 142 (1980) 467-473.
2. Alexander M, Biodegradation of chemicals of environmental concern, Science, 211 (1981) 132-138.
3. Klages U & Lingens F, Degradation of 4-chlorobenzoic acid by Nocardia species, FEMS Microbiol Lett, 6 (1979) 201-203.
4. Hartmann J, Reineke W & Knackmuss H J, Metabolism of 3-chloro, 4-chloro and 3,5-dichloróbenzoate by pseudomonad, Appl Environ Microbiol, 37 (1979) 421-428.
5. Latorre J, Reineke W & Knackmuss H J, Microbial metabolism of chloroaniline: Enhanced evolution by natural genetic exchange, Arch Microbiol, 140 (1984) 159-165.
6. Lehrbach P R, Zeyer J, Reineke W, Knackmuss H J & Timmis K N, Enzymatic recruitment in vitro: Use of cloned genes to extend the range of haloaromatics degraded by Pseudomonas sp. Strain B13, J Bacteriol, 158 (1984) 1025-1032.
7. Rubio M A, Engesser K H & Knackmuss H J, Microbial metabolism of chlorosalicylates: Accelerated evolution by natural genetic-exchange, Arch Microbiol, 145 (1986) 116-122.
8. Thakur I S, Structural and functional characterization of a stable, 4-chlorosalicylic acid-degrading, bacterial community in a chemostat, World J Microbiol Biotechnol, 11 (1995) 643-645.
9. Marks T S, Smith A R W & Quirk A V, Degradation of 4-chlorobenzoic acid by Arthrobactor sp, Appl Environ Microbiol, 48 (1984) 1020-1025.
10. Thakur I S, Biodegradation of chlorinated benzoic acid by Pseudomonas fluorescens from a chemostat, Indian J Microbiol, 41 (2001) 27-32.
11. Bergman J G & Sanik J Jr., Determination of trace amount of naphtha, Anal Chem, 29 (1957) 241-243.
12. Holding A J & Colle J G, Routine biochemical test, in Methods in microbiology, vol 6A, edited by J R Norris and D W Ribbons (Academic Press, London) 1971, 1-32.
13. Nakazawa T & Nakazawa A, Pyrocatechase (Pseudomonas), in Methods in enzymology, edited by H Tabor and C Tabor (Academic Press, New York), Vol 37A, 1970, 518-520.
14. Dorn E & Knackmuss H J, Chemical structure and biodegradability of halogenated aromatic compounds: Substituent effects on 1,2-dioxygenation of catechol, Biochem J, 174 (1978) 85-94.
15. Nozaki M, Metapyrocatechase (Pseudomonas), in Methods in enzymology, edited by H Tabor and C Tabor (Academic Press, New York), Vol 37A, 1970, 522-525.
16. Lowry O H, Rosebrough N J, Farr A C & Randall R J, Protein measurement with Folin-phenol reagent, J Biol Chem, 193 (1951) 265-275.
17. Thakur I S, Fractionation and analysis of allergenicity of allergens from Prososis juliflora pollen, Int Arch Allergy Appl Immunol, 90 (1989) 124-129.
18. Laemmli U K, Cleavage of structural protein during the assembly of the head of bacteriophage T4, Nature (Lond), 227 (1970) 680-685.
19. Kobayashi K, Katayama-Hirayama K & Tobita S, Hydrolytic dehalogenation of 4-chlorobenzoic acid by an Acinetobacter sp, J Gen Appl Microbiol, 43 (1997) 105-108.
20. Löffter F, Müller R & Lingens F, Dehalogenation of 4-chlorobenzoate by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3: An ATP/coenzyme A dependent reaction, Biochem Biophys Res Coomun, 176 (1991) 1106-1110.