Chimerization of multiple antibody classes using splice overlap extension PCR

BioTechniques

Volumn 38, Issue 2, 2005, Pages 181-182

  Jones, Martina L ^a   Barnard, Ross T ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; MONOCLONAL ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; CHIMERA; DNA MODIFICATION; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HYBRIDOMA; NONHUMAN; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT;

EID: 13444249491     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05382BM01     Document Type: Article

References (4)

1. Horton, R.M., H.D. Hunt, S.N. Ho, J.K. Pullen, and L.R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68.
2. Stewart, L.M., S. Young, G. Watson, S.J. Mather, P.A. Bates, H.A. Band, R.W. Wilkinson, E.L. Ross, and D. Snary. 1999. Humanisation and characterisation of PR1A3, a monoclonal antibody specific for cell-bound carcinoembryonic antigen. Cancer Immunol. Immunother. 47:299-306.
3. Lefranc, M.P. 2003. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 31:307-310.
4. Coloma, M.J., A. Hastings, L.A. Wims, and S.L. Morrison. 1992. Novel vectors for the expression of antibody molecules using variable regions generated by polymerase chain reaction. J. Immunol. Methods 152:89-104.