Recombinant Expression, Biochemical Characterization and Stabilization through Proteolysis of an L-Glutamate Oxidase from Streptomyces sp. X-119-6

Journal of Biochemistry

Volumn 134, Issue 6, 2003, Pages 805-812

  Arima, Jiro ^a   Tamura, Takashi ^a   Kusakabe, Hitoshi ^b   Ashiuchi, Makoto ^c   Yagi, Toshiharu ^c   Tanaka, Hidehiko ^a   Inagaki, Kenji ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b YAMASA CORPORATION   (Japan)

^c KOCHI UNIVERSITY   (Japan)

Author keywords

L glutamate oxidase; Precursor form; Proteolysis; Streptomyces sp.

Indexed keywords

ENZYME PRECURSOR; GLUTAMATE OXIDASE; METALLOPROTEINASE; OXIDOREDUCTASE; POLYPEPTIDE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME ISOLATION; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBUNIT; ESCHERICHIA COLI; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PROTEIN AGGREGATION; PROTEIN EXPRESSION; STREPTOMYCES;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; ENZYME PRECURSORS; ENZYME STABILITY; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HYDROLYSIS; MOLECULAR SEQUENCE DATA; PEPTIDE HYDROLASES; RECOMBINANT PROTEINS; STREPTOMYCES;

ESCHERICHIA COLI; STREPTOMYCES; STREPTOMYCES GRISEUS; STREPTOMYCES SP.; STREPTOMYCES SP. X-119-6;

EID: 1342346743     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvg206     Document Type: Article

References (26)

1. Cooper, J.M., Calum, M.J., and Julia, A.S. (1991) Amperometric enzyme electrode for the determination of aspartate aminotransferase and alanine aminotransferase is serum. Anal. Chim. Acta 245, 57-62
2. Kamei, T., Asano, K., Suzuki, H., Matsuzaki, M., and Nakamura, S. (1983) L-Glutamate oxidase from Streptomyces violascens. I. Production, isolation and some properties. Chem. Pharm. Bull. 31, 1307-1314
3. Kamei, T., Asano, K., Kondo, H., Matsuzaki, K., and Nakamura, S. (1983) L-Glutamate oxidase from Streptomyces violascens. II. Properties. Chem. Pharm. Bull. 31, 3609-3616
4. Bohmer, A., Muller, A., Passarge, M., Liebs, P., Honeck, H., and Muller, H.G. (1989) A novel L-glutamate oxidase from Streptomyces endus. Purification and properties. Eur. J. Biochem. 15, 327-332
5. Kusakabe, H., Midorikawa, Y., Fujishima, T., Kuninaka, A., and Yoshino, H. (1983) Purification and properties of a new enzyme, L-glutamate oxidase, from Streptomyces sp. X-119-6 grown on wheat bran. Agric. Biol. Chem. 47, 1323-1328
6. Chen, C.Y., Wu, W.T., Huang, C.J., Lin, M.H., Chang, C.K., Huang, H.J., Liao, J.M., Chen, L.Y., and Liu, Y.T. (2001) A common precursor for the three subunits of L-glutamate oxiase encoded by gox gene from Streptomyces platensis NTU3304. Can. J. Microbiol. 47, 269-275
7. Short, J.M., Fernandez, J.M., Sorge, J.A., and Huse, W.D. (1988) Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res. 11, 7583-600
8. Amann, E., Brosius, J., and Ptashne, M. (1983) Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25, 167-178
9. Yanisch, P.C., Vieira, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119
10. Hopwood, D.A., Bibb, M.J., Chater, K.F., Kieser, T., Thompson, C.J., Kieser, H.M., Lydite, D.J., and Schrempf, H. (1985) Genetic Manipulation of Streptomyces: a Laboratory Manual, John Innes Foundation, Norwich, UK
11. Maniatis, T., Fritsch, F.F., and Sambrook, J. (1989) Molecular cloning: a Laboratory Manual, 2nd ed., Cold Spring Harbor Lab., Cold Spring Harbor, New York
12. Saito, H. and Miura, K. (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72, 619-629
13. Birnboim, H.C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 24, 1513-1523
14. Southern, E.M. (1975) Detection of specific sequence among DNA fragment separated by gel electrophoresis. J. Mol. Biol. 98, 503-517
15. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA 74, 5463-5467
16. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402
17. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 15, 680-685
18. Feinberg, A.P. and Vogelstein, B. (1983) A technique for radiolabelling DNA restriction endonuclease fragment to high specific activity. Anal. Biochem. 132, 6-13
19. Short, J.M. and Sorge, J.A. (1992) In vivo excision properties of bacteriophage lambda ZAP expression vectors. Methods Enzymol. 216, 495-508
20. Allain, C.C., Poon, L.S., Chan, C.S., Richmond, W., and Fu, P.C. (1974) Enzymatic determination of total serum cholesterol. Clin. Chem. 20, 470-475
21. Soda, K. (1968) Microdetermination of D-amino acids and D-amino acid oxidase activity with 3-methyl-2-benzothiazolone hydrazone hydrochloride. Anal. Biochem. 24, 228-235
22. Segel, I.H. (1976) Biochemical Calculations, pp. 277-281, John Wiley and Sons, New York
23. Wierenga, R.K., Terpstra, P., and Hol, W.G. (1986) Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint. J. Mol. Biol. 187, 101-107
24. Pawelek, P.D., Cheah, J., Coulombe, R., Macheroux, P., Ghisla, S., and Vrielink, A. (2000) The structure of L-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site. EMBO J. 19, 4204-4215
25. MacHeroux, P., Seth O., Bollschweiler, C., Schwarz, M., Kurfurst, M., Au, L.C., and Ghisla, S. (2001) L-Amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma. Comparative sequence analysis and characterization of active and inactive forms of the enzyme. Eur. J. Biochem. 268, 1679-1686
26. Binda, C., Coda, A., Angelini, R., Federico, R., Ascenzi, P., and Mattevi, A. (1999) A 30 A long U-shaped catalytic tunnel in the crystal structure of polyamine oxidase. Structure 7, 265-276