Does negative heparin-platelet factor 4 enzyme-linked immunosorbent assay effectively exclude heparin-induced thrombocytopenia?

Journal of Thrombosis and Haemostasis

Volumn 1, Issue 8, 2003, Pages 1844-1845

  Fohlen Walter, A ^a,b   De Maistre, E ^a,c   Mulot, A ^a   Marchand Arvier, M ^a,b,c   Lecompte, T ^a,b,c  

^a UNIVERSITY HOSPITAL OF NANCY   (France)

^b UNIVERSITÉ DE LORRAINE   (France)

^c UNIVERSITÉ DE LORRAINE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTICOAGULANT AGENT; HEPARIN; THROMBOCYTE FACTOR 4;

BIOSYNTHESIS; CHEMICALLY INDUCED DISORDER; CLINICAL CHEMISTRY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; METHODOLOGY; NOTE; PROTEIN BINDING; THROMBOCYTOPENIA;

ANTICOAGULANTS; CHEMISTRY, CLINICAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEPARIN; HUMANS; LABORATORY TECHNIQUES AND PROCEDURES; PLATELET FACTOR 4; PROTEIN BINDING; THROMBOCYTOPENIA;

EID: 1342336161     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1046/j.1538-7836.2003.00282.x     Document Type: Letter

References (20)

1. Warkentin TE, Chong BH, Greinacher A. Heparin-induced thrombocytopenia: towards consensus. Thromb Haemost 1998; 79: 1-7.
2. Reilly MP, McKenzie SE. Insights from mouse models of heparin-induced thrombocytopenia and thrombosis. CurrOpinHematol2002; 9:395^4-00.
3. Eichler P, Budde U, Haas S, Kroll H, Loreth RM, Meyer O, Pachmann U, Potzsch B, Schabel A, Albrecht D, Greinacher A. First workshop for detection of heparin-induced antibodies: validation of the heparin-induced platelet-activation test (HIPA) in comparison with a PF4/ heparin ELISA. Thromb Haemost 1999; 81: 625-9.
4. Deeks JJ. Systematic reviews of evaluations of diagnostic and screening tests. BMJ 2001; 323: 157-62.
5. Amiral J, Bridey F, Dreyfus M, Vissac AM, Fressinaud E, Wolf M, Meyer D. Platelet factor 4 complexed to heparin is the target for antibodies generated in heparin-induced thrombocytopenia. Thromb Haemost 1992; 68: 95-6.
6. Greinacher A, Potzsch B, Amiral J, Dummel V, Eichner A, Muller-Eckardt C. Heparin-associated thrombocytopenia: isolation of the anti body and characterization of the multimolecular PF4-heparin complex as the major antigen. Thromb Haemost 1994; 71: 247-51.
7. Amiral J, Bridey F, Wolf M, Boyer-Neumann C, Fressinaud E, Vissac AM, Peynaud-Debayle E, Dreyfus M, Meyer D. Antibodies to macromolecular platelet factor 4-heparin complexes in heparin-induced thrombocytopenia. a study of 44 cases. Thromb Haemost 1995; 73: 21-8.
8. Arepally G, Reynolds C, Tomaski A, Amiral J, Jawad A, Poncz M, Cines DB. Comparison of PF4/heparin ELISA assay with 14C-serotonin release assay in the diagnosis of heparin-induced thrombocytopenia. Am J Clin Pathol 1995; 104: 648-54.
9. Amiral J, Wolf M, Marfaing-Koka A, Vissac AM, Boyer-Neumann C, Meyer D. Characteristics of antibodies to PF4, IL-8 and NAP-2 complexed to heparin in patients with heparin-induced thrombocyto penia: a study of 187 cases. Thromb Haemost 1997; (Suppl.): 449.
10. Samama MM, Elalamy I, Lecrubier C, Potevin F, Horellou MH, Conard J. Thrombopenies induites par l'heparine: interet et difficultes de 1'identification precise d'un mecanisme immunologique. Bull Acad Natle Med 1998; 182: 1517-36.
11. Pouplard C, Amiral J, Borg JY, Laporte-Simitsidis S, Delahousse B, Gruel Y. Decision analysis for use of platelet aggregation test, carbon-14 serotonine release assay, and heparin-platelet factor 4 enzyme-linked immunosorbent assay for diagnosis of heparin-induced thrombocyto penia. Am J Clin Pathol 1999; 111: 700-6.
12. Walenga JM, Jeske WP, Wood JJ, Ahmad S, Lewis BE, Bakhos M. Laboratory tests for heparin-induced thrombocytopenia: a multicenter study. Semin Haematol 1999; 36 (Suppl. 1): 22-8.
13. Rugeri L, Bauters A, Trillot N, Susen S, Decoen C, Watel A, Jude B. Clinical usefulness of combined use of platelet aggregation test and anti PF4-H. Antibodies ELISA test for the diagnosis of heparin induced thrombocytopenia. Hematology 1999; 4: 367-72.
14. Gruel Y, Nguyen P, Pouplard C, Borg JY, Derlon A, Juhan-Vague I, Boudignat O, Regnault V, Samama M. Is low molecular weight heparin-induced thrombocytopenia different from HIT due to unfractionated heparin? Blood 2000; 96: 272a.
15. Lindhoff-Last E, Gerdsen F, Ackermann H, Bauersachs R. Determina tion of heparin-platelet factor 4-IgG antibodies improves diagnosis of heparin-induced thrombocytopenia. Br J Haematol 2001; 113: 886-90.
16. George JN, Raskob GE, Shah SR, Rizvi MA, Hamilton SA, Osborne S, Vondracek T. Drug-induced thrombocytopenia: a systematic review of published case reports. Ann Intern Med 1998; 129: 886-90.
17. Magid E. Minimal requirements for test evaluation. Scand J Clin Lab Invest 1997; 57 (Suppl. 227): 90-4.
18. Amiral J, Marfaing-Koka A, Wolf M, Alessi MC, Boyer-Neumann C, Vissac AM, Fressinaud E, Poncz M, Meyer D. Presence of autoanti-bodies to interleukin-8 or neutrophil-activating peptide-2 in patients with heparin-associated thrombocytopenia. Blood 1996; 88: 410-6.
19. Regnault V, de Maistre E, Carteaux JP, Gruel Y, Nguyen P, Tardy B, Lecompte T. Platelet activation induced by human antibodies to inter leukin-8. Blood 2003; 101: 1419-21.
20. Harenberg J, Wang LC, Hoffmann U, Huhle G, Feuring M. Laboratory diagnosis of heparin-induced thrombocytopenia type II after clearance of platelet factor 4/heparin complex. JLab ClinMed200l;137:408-13.