A substrate-induced switch in the reaction mechanism of a thermophilic esterase: Kinetic evidences and structural basis

Journal of Biological Chemistry

Volumn 279, Issue 8, 2004, Pages 6815-6823

  De Simone, Giuseppina ^a   Mandrich, Luigi ^b   Menchise, Valeria ^a   Giordano, Valeria ^a   Febbraio, Ferdinando ^b   Rossi, Mosè ^b   Pedone, Carlo ^a   Manco, Giuseppe ^b  

^a CNR   (Italy)

^b INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOLS; ENZYME INHIBITION; ENZYMES; MUTAGENESIS; REACTION KINETICS; STRUCTURAL ANALYSIS;

KINETIC CONSTANTS;

BIOCHEMISTRY;

1 HEXADECANESULFONYL CHLORIDE; ESTERASE; ESTERASE II; HEXADECANE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING SITE; COVALENT BOND; CRYSTAL STRUCTURE; DERIVATIZATION; DETOXIFICATION; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROLYSIS; LIPOLYSIS; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL; THERMOPHILIC BACTERIUM;

ALCOHOLS; BACTERIA; BINDING SITES; CAPROATES; CARBOXYLIC ESTER HYDROLASES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; DOSE-RESPONSE RELATIONSHIP, DRUG; KINETICS; LAURATES; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTATION; NITROBENZENES; PROTEIN BINDING; SUBSTRATE SPECIFICITY; SULFONES; TEMPERATURE; TIME FACTORS;

ANIMALIA; BACTERIA (MICROORGANISMS); HUMAN ECHOVIRUS 1;

EID: 1342304115     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M307738200     Document Type: Article

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