Characterization of Osteocalcin (BGP) and Matrix Gla Protein (MGP) Fish Specific Antibodies: Validation for Immunodetection Studies in Lower Vertebrates

Calcified Tissue International

Volumn 74, Issue 2, 2004, Pages 170-180

  Simes, D C ^a   Williamson, M K ^b   Schaff, B J ^a   Gavaia, P J ^a   Ingleton, P M ^c   Price, P A ^b   Cancela, M L ^a  

^a UNIVERSITY OF ALGARVE   (Portugal)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MATRIX GLA PROTEIN; OSTEOCALCIN; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ANTIBODY SPECIFICITY; ARTICLE; BONE REMODELING; EXTRACELLULAR MATRIX; FISH; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; SHARK; TELEOST; VERTEBRATE; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODY SPECIFICITY; BONE AND BONES; CALCIUM-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXTRACELLULAR MATRIX PROTEINS; FISHES; MOLECULAR SEQUENCE DATA; OSTEOCALCIN; SPECIES SPECIFICITY; XENOPUS;

EID: 1342302340     PISSN: 0171967X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00223-003-0079-4     Document Type: Article

References (30)

1. Hauschka PV, Lian JB, Cole DEC, Gundberg CM (1989) Osteocalcin and matrix Gla protein: vitamin K-dependent proteins in bone. Physiol Rev 69:990-1047
2. Ducy P, Desbois C, Boyce B, et al. (1996) Increased bone formation in osteocalcin-deficient mice. Nature 382:448-452
3. Luo G, Ducy P, McKee MD, Pinero GJ, Loyer E, Behringer RR, Karsenty G (1997) Spontaneous calcification of arteries and cartilage in mice lacking matrix gla protein. Nature 386:78-81
4. Yagami K, Suh J-Y, Enomoto-Iwamoto M, Koyama E, Abrams WR, Shapiro IM, Pacifici M, Iwamoto M (1999) Matrix gla protein is a developmental regulator of chondrocyte mineralization and, when constitutively expressed, blocks endochondral and intramembranous ossification in the limb. J Cell Biol 147:1097-1108
5. Shanahan CM, Proudfoot D, Farzaneh-Far A, Weissberg PL (1998) The role of Gla proteins in vascular calcification. Crit Rev Eukaryotic Gene Express 8:357-375
6. Price PA, Faus SA, Williamson MK (1998) Warfarin causes rapid calcification of the elastic lamellae in rat arteries and heart valves. Arterioscler Thromb Vasc Biol 18:1400-1407
7. Shanahan CM, Cary NRB, Salisbury JR, Proudfoot D, Weissberg PL, Edmonds M (1999) Medial localization of mineralization-regulating proteins in association with monckeberg's sclerosis, evidence for smooth cell-mediated vascular calcification. Circulation 100:2168-2176
8. Price PA, Faus SA, Williamson MK (2000) Warfarin-induced artery calcification is accelerated by growth and vitamin D. Arterioscler Thromb Vasc Biol 20:317-327
9. Shanahan CM, Proudfoot D, Tyson KL, Cary NRB, Edmonds M, Weissberg PL (2000) Expression of mineralization-regulating proteins in association with human vascular calcification. Z Kardiol 89:63-68
10. Spronk HMH, Soute BAM, Schurgers LJ, Cleutjens JPM, Thijssen HHW, De Mey JGR, Vermeer C (2001) Matrix gla protein accumulates at the border of regions of calcification and normal tissue in the media of the arterial vessel wall. Biochem Biophys Res Commun 289:485-490
11. Otawara Y, Price PA (1986) Developmental appearance of Matrix gla protein during calcification in rat. J Biol Chem 261:10828-10832
12. Hale JE, Mattew KW, Price PA (1991) Carboxyl-terminal proteolytic processing of matrix gla protein. J Biol Chem 266:21145-21149
13. Price PA, Rice JS, Williamson MK (1994) Conserved phosphorylation of serine in the Ser-X-Glu/Ser(P) sequences of the vitamin K-dependent matrix gla protein from shark, lamb, rat, cow and human. Protein Sci 3:822-830
14. Cancela ML, Williamson MK, Ohresser MCP, Reia JP, Price PA (2001) Matrix gla protein in Xenopus laevis: molecular cloning, tissue distribution and evolutionary considerations. J Bone Miner Res 16:1611-1621
15. Simes DC, Williamson MK, Ortiz-Delgado JB, Viegas CSB, Cancela ML, Price PA (2003) Purification of Matrix Gla Protein (MGP) from a marine teleost fish, Argyrosomus regius: calcified cartilage and not bone as the primary site of MGP accumulation in fish. J Bone Miner Res 18:244-259
16. Nishimoto SK, Araki N, Robinson FD, Waite JH (1992) Discovery of bone γ-carboxyglutamic acid protein in mineralized scales. The abundance and structure of Lepomis macrochirus bone γ-carboxyglutamic acid. J Biol Chem 267:11600-11605
17. Cancela ML, Williamson MK, Price PA (1995) Amino-acid sequence of Bone Gla Protein from the African clawed toad Xenopus laevis and the fish Sparus aurata. Int J Peptide Protein Res 46:419-423
18. Delmas PD, Christiansen C, Mann KG, Price PA (1990) Bone Gla Protein (osteocalcin) assay standardization report. J Bone Miner Res 5:5-11
19. Jie K-SG, Gijsbers BLMG, Vermeer C (1995) A specific colorimetric staining method for γ-carboxyglutamic acid-containing proteins in polyacrylamide gels. Anal Biochem 224:163-165
20. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
21. Rice JS, Williamson MK, Price PA (1994) Isolation and sequence of the vitamin K-dependent Matrix Gla Protein from the calcified cartilage of the soupfin shark. J Bone Miner Res 9:567-576
22. Poser JW, Price PA (1979) A method for decarboxylation of the γ-carboxyglutamic acid in proteins. J Biol Chem 254:431-436
23. 3 treatment of osteosarcoma cells. Calcif Tissue Int 46:270-279
24. 3 stimulates the synthesis of matrix γ-carboxyglutamic acid protein by osteosarcoma cells. J Biol Chem 263:911-916
25. Price PA, Otsuka AS, Poser JW, Kristaponis J, Raman N (1976) Characterization of a γ-carboxyglutamic acid-containing protein from bone. Proc Natl Acad Sci USA 73:1447-1451
26. 2+ binding. In: Wasserman RH, Corradino RA, Carafoli E, Kretsinger RH, Mac-Lennan DH, Siegel FL (Eds.) Calcium-binding proteins and calcium function. Elsevier North-Holland, Amsterdam, pp 333-337
27. Witten PE (1997) Enzyme histochemical characteristics of osteoblasts and mononucleated osteoclasts in a teleost fish with acellular bone (Oreochromis niloticus, Cichlidae). Cell Tissue Res 287:591-599
28. Witten PE, Hansen A, Hall BK (2001) Features of mono-and multinucleated bone-resorbing cells of the Zebrafish Danio rerio and their contribution to skeletal development, remodeling and growth. J Morph 250:197-207
29. Price PA, Poser JW, Raman N (1976) Primary structure of the gamma-carboxyglutamic acid-containing protein from bone. Proc Natl Acad Sci USA 73:3374-3375
30. Viegas CS, Pimto JP, Conceicao N, Simes DC, Cancela ML (2002) Cloning and characterization of the cDNA and gene encoding Xenopus laevis osteocalcin Gene 289:97-107