One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus

Biotechnology Letters

Volumn 26, Issue 3, 2004, Pages 179-183

  Tao, Sheng Ce ^a   Jiang, Di ^b   Lu, Hong Li ^b   Xing, Wan Li ^a,b   Zhou, Yu Xiang ^a,b   Cheng, Jing ^a,b  

^a TSINGHUA UNIVERSITY   (China)

^b NATIONAL ENGINEERING RESEARCH CENTER FOR BEIJING BIOCHIP TECHNOLOGY   (China)

Author keywords

One tube nested PCR; Plastic film; SRAS virus

Indexed keywords

BIOMATERIAL; POLYETHYLENE DERIVATIVE; VIRUS RNA;

ARTICLE; COMPARATIVE STUDY; EQUIPMENT; EQUIPMENT DESIGN; EVALUATION; GENETICS; INSTRUMENTATION; ISOLATION AND PURIFICATION; METHODOLOGY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SARS CORONAVIRUS; SENSITIVITY AND SPECIFICITY;

BIOCOMPATIBLE MATERIALS; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; POLYETHYLENES; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SARS VIRUS; SENSITIVITY AND SPECIFICITY;

CORONAVIRUS; SARS CORONAVIRUS;

EID: 1342288694     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000013708.65032.f1     Document Type: Article

References (10)

1. Abath FG, Melo FL, Werkhauser RP, Montenegro L, Montenegro R, Schindler HC (2002) Single-tube nested PCR using immobilized internal primers. Biotechniques 33: 1210-1212, 1214.
2. Dupin N, Buffet M, Marcelin AG, Lamotte C, Gorin I, Ait-Arkoub, Z, Treluyer JM, Bui P, Calvez V, Peytavin G (2002) HIV and antiretroviral drug distribution in plasma and fat tissue of HIV-infected patients with lipodystrophy. AIDS 16: 2419-2424.
3. Garson JA, Tuke PW, Makris M, Briggs M, Machin SJ, Preston FE, Tedder RS (1990) Demonstration of viraemia patterns in haemophiliacs treated with hepatitis-C-virus-contaminated factor VIII concentrates. Lancet 336: 1022-1025.
4. Llop P, Bonaterra A, Penalver J, Lopez MM (2000) Development of a highly sensitive nested-PCR procedure using a single closed tube for detection of Erwinia amylovora in asymptomatic plant material. Appl. Environ. Microbiol. 66: 2071-2078.
5. Mathis A, Weber R, Kuster H, Speich R (1997) Simplified sample processing combined with a sensitive one-tube nested PCR assay for detection of Pneumocystis carinii in respiratory specimens. J. Clin. Microbiol. 35: 1691-1695.
6. Olmos A, Cambra M, Esteban O, Gorris MT, Terrada E (1997) New device and method for capture, reverse transcription and nested PCR in a single closed-tube. Nucl. Acids Res. 27: 1564-1565.
7. Pignon JM, Henni T, Amselem S, Vidaud M, Duquesnoy P, Vernant JP, Kuentz M, Cordonnier C, Rochant H, Goossens M (1990) Frequent detection of minimal residual disease by use of the polymerase chain reaction in long-term survivors after bone marrow transplantation for chronic myeloid leukemia. Leukemia 4: 83-86.
8. Porter-Jordan K, Rosenberg EI, Keiser JF, Gross JD, Ross AM, Nasim S, Garrett CT (1990) Nested polymerase chain reaction assay for the detection of cytomegalovirus overcomes false positives caused by contamination with fragmented DNA. J. Med. Virol. 30: 85-91.
9. Tanji Y, Mizoguchi K, Akitsu T, Monta M, Hori K, Unno H (2002) Fate of coliphage in waste water treatment process and detection of phages carrying the Shiga toxin type 2 gene. Water Sci. Technol. 46: 285-289.
10. Wolff C, Hornschemeyer D, Wolff D, Kleesiek K (1995) Single-tube nested PCR with room-temperature-stable reagents. PCR Meth. Appl. 4: 376-379.