Novel, ligation-dependent PCR assay for detection of hepatitis C virus in serum

Journal of Clinical Microbiology

Volumn 34, Issue 3, 1996, Pages 501-507

  Hsuih, Terence Chun Hung ^a   Park, Young Nyun ^a   Zaretsky, Craig ^a   Wu, Fann ^a   Tyagi, Sanjay ^b   Kramer, Fred Russell ^b   Sperling, Rhoda ^a   Zhang, David Yong ^a  

^a MOUNT SINAI HOSPITAL   (United States)

^b PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYDEOXYRIBONUCLEOTIDE SYNTHASE; VIRUS RNA;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DNA PROBE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA ANALYSIS; VIRUS DETECTION;

EID: 13344294387     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.3.501-507.1996     Document Type: Article

References (5)

1. Barany, F. 1991. Genetic disease detection and DNA amplification using cloned thermostable ligase. Proc. Natl. Acad. Sci. USA 88:189-193.
2. Cha, T. A., J. Kolberg, B. Irvine, M. Stempien, E. Beall, M. Yano, A. L. Choo, M. Houghton, G. Kuo, J. H. Han, and M. S. Urdea. 1991. Use of a signature nucleotide sequence of hepatitis C virus for detection of viral RNA in human serum and plasma. J. Clin. Microbiol. 29:2528-2534.
3. Chang, G. J., D. W. Trent, A. V. Vorndam, E. Vergne, R. M. Kinne, and C. J. Mitchell. 1994. An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses. J. Clin Microbiol. 32:477-483.
4. Chirgwin, J. M., A. E. Przybyia, R. J. MacDonald, and W. J. Rutter. 1979. Isolation of biologically active RNA from sources enriched in ribonuclease. Biochemistry 18:5294-5299.
5. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidimum thiocyanate-phenol-chloroform extraction. Anal. Biochem, 162:156-159.